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Hernández Rodríguez, Martha. |
El maíz (Zea mays L.) de calidad proteínica (QPM) es nutricionalmente superior en comparación con el maíz normal, porque produce semillas con casi el doble de los aminoácidos (aa) lisina (Lys) y triptófano (Trp), esenciales para la síntesis endógena de la vitamina B niacina. Actualmente, la conversión de una línea de maíz normal a una QPM, se acelera mediante la selección asistida por marcadores moleculares (MAS) y métodos de detección química. En este estudio se reporta el desarrollo y validación de marcadores basados en polimorfismos de un solo nucleótido (SNPs) para los loci opaque-2 (o2) y Ask-2, involucrados en la genética de los QPM, así como la interacción de ambos loci para el contenido de 11 aa en el endospermo de dos poblaciones F2 de maíz,... |
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Palavras-chave: Zea mays; Opaque-2; Ask-2; MAS; QPM; SNP; SSCP; Genética; Doctorado. |
Ano: 2013 |
URL: http://hdl.handle.net/10521/2319 |
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Montaldo,Hugo H.. |
This paper discusses the use of genetic engineering applications in animal breeding, including a description of the methods, their potential and current uses and ethical issues. Genetic engineering is the name of a group of techniques used to identify, replicate, modify and transfer the genetic material of cells, tissues or complete organisms. Important applications of genetic engineering in animal breeding are: 1) Marker-assisted selection (MAS). The objective of this technology is to increase disease resistance, productivity and product quality in economically important animals by adding information of DNA markers to phenotypes and genealogies for selection decisions. 2) Transgenesis, the direct transfer of specific genes/alleles between individuals,... |
Tipo: Journal article |
Palavras-chave: Adult mammalian cloning; Biotechnology; Gene mapping; GMOS; MAS; QTL; Transgenics. |
Ano: 2006 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000200010 |
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Silva,Marcia Flores da; Schuster,Ivan; Silva,João Flávio Veloso da; Ferreira,Adésio; Barros,Everaldo Gonçalves de; Moreira,Maurilio Alves. |
The objective of this work was to validate microsatellite markers associated with resistance to soybean cyst nematode (Heterodera glycines Ichinohe) races 3 and 14, in soybean (Glycine max L.) genotypes, for use in marker-assisted selection (MAS) programs. Microsatellites of soybean linkage groups A2, D2 and G were tested in two populations, and their selection efficiencies were determined. The populations were 65 F2:3 families from Msoy8001 (resistant) x Conquista (susceptible) cross, and 66 F2:3 families of S5995 (resistant) x Renascença (susceptible) cross, evaluated for resistance to races 3 and 14, respectively. Families with female index up to 30% were considered moderately resistant. Markers of A2 and G linkage groups were associated with resistance... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glycine max; Heterodera glycines; Soybean breeding; SCN; SSR; MAS. |
Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000800011 |
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