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| Vasconcellos,Isabelle; Mariani,Diana; Azevedo,Marcelo C.V.M. de; Ferreira Jr.,Orlando C.; Tanuri,Amilcar. |
| ABSTRACT A new point-of-care HIV viral load, mPIMA HIV-1/2 VL, Abbott, USA, has been recently developed. This point-of-care viral load requires no skilled person to run and uses a small plasma volume (50 µL). However, obtaining 50 µL of plasma can be a challenge in limited resource settings. We validated a simple and easy method to obtain enough amount of plasma to run a point-of-care viral load. The study utilized 149 specimens from patients failing antiretroviral therapy. At least 250 µL of whole blood was collected in a microtube/EDTA from fingerstick (fs-plasma) and immediately centrifuged. Parallel collection of venous blood to obtain plasma (vp-plasma) was used to compare performance in a point-of-care viral load assay and in methodology used in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: POC VL; HIV viral load; HIV; Point-of-care; Fingerstick-plasma; MPIMA. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702020000100030 |
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