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In vitro micropropagation of Stevia rebaudiana Bertoni in Malaysia BABT
Razak,Ummi Nur Ain Abdul; Ong,Chong Boon; Yu,Tiew Sing; Lau,Li Kiaw.
Stevia rebaudiana Bertoni is a medicinal plants and commercially use as non-caloric sweetener for diabetic patient. In the present study, a protocol was developed for in vitro micropropagation using 6-benzylamino purine (BAP) and Kinetin (Kn) for the formation of multiple shoot proliferation and Indole-3-acetic acid (IAA), Indole-3-butyric acid (IBA) and 1-Naphthaleneacetic acid (NAA) for the induction of roots. Maximum shoot formation (7.82 ± 0.7 shoots per explants) was observed on a Murashige and Skoog (MS) medium supplemented with 0.5 mg L-1 BAP and 0.25 mg L-1 Kn. The maximum number of roots (30.12 ± 2.1 roots per explants) was obtained on a MS medium containing 1.0 mg L-1 IBA. The well rooted plantlets were successfully weaned and acclimatized in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MS medium; Non-caloric sweetener; Proliferation.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000100004
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Germination and germ tube growth of the arbuscular mycorrhizal fungi Gigaspora albida in different substrates BJM
Maia,Leonor C.; Yano-Melo,Adriana M..
The role of the substrate on germination of Gigaspora albida Schenck & Smith was investigated. Spores were desinfested with 0.5% sodium hypochlorite (20 min.) and placed on Petri dishes over a Millipore filter, with one of the following media: a- 1% water-agar; b- water-agar + aqueous extract of roots of Panicum miliaceum L.; c- salt medium of Murashige & Skoog (MS) or sterilized sand; and incubated in the dark at room temperature (28ºC ± 2). The experimental design was at random with four treatments and four replicates. Germination was evaluated every 7 days until the 28th day. The water-agar medium was the most feasible for spore germination at the 7th day, followed by the MS medium at the 14th day. Conversely, the sand and the root...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Water-agar; MS medium; Root extract; Glomales; Panicum miliaceum.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400005
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Irradiation effect on in vitro organogenesis, callus growth and plantlet development of Gerbera jamesonii Horticultura Brasileira
Hasbullah,Nor A; Taha,Rosna M; Saleh,Azani; Mahmad,Noraini.
The present work was carried out to study the effects of gamma irradiation on in vitro growth of explants, callus and the formation of shoots and plantlets. Irradiation is known to exhibit or inhibit the differentiation of cells and growth of plants in vitro, which helps in producing new plant varieties. Gamma irradiation is one of the physical mutagens that are widely used for mutation breeding. A gradual decline was observed in the number of shoots regenerated from irradiated petiole explants compared to control. Numbers of shoots regenerated from irradiated petiole explant cultured on Murashige & Skoog medium supplemented with 2.0 mg L-1 BAP and 0.5 mg L-1 NAA was reduced to 6.6±0.9 from 7.5±0.4 (control) when explants were exposed to 20 Gray of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gerbera jamesonii; Ornamental; Gamma irradiation; Explant; Callus regeneration; Plant growth regulator; MS medium.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362012000200012
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