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| Martos,María Alicia; Zubreski,Emilce Roxana; Combina,Mariana; Garro,Oscar Alfredo; Hours,Roque Alberto. |
| The objective of the present study was the isolation of a yeast strain, from citrus fruit peels, able to produce a polygalacturonase by submerged fermentation with maceration activity of raw cassava roots. Among 160 yeast strains isolated from citrus peels, one strain exhibited the strongest pectinolytic activity. This yeast was identified as Wickerhamomyces anomalus by 5.8S-ITS RFLP analysis and confirmed by amplification of the nucleotide sequence. The yeast produced a polygalacturonase (PG) in Erlenmeyer shake flasks containing YNB, glucose, and citrus pectin. PG synthesis occurred during exponential growth phase, reaching 51 UE.mL-1 after 8 hours of fermentation. A growth yield (Yx/s) of 0.43 gram of cell dry weight per gram of glucose consumed was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Wickerhamomyces anomalus; Polygalacturonase; Maceration; Cassava. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000200018 |
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| Jorquera,Alejandro Plascencia; Ponce,José Gerardo Serrano; Sánchez-Mendoza,Berenice; Serrano,Alberto Barreras; Calderón-Cortés,José Fernando; López-Soto,María Alejandra; Montaño Gómez,Martín Francisco; Zinn,Richard Avery. |
| Mechanical maceration enhances site and extent of digestion of low-moisture, low-quality forages. Four Holstein steers (172 ± 8 kg) with cannulas in rumen and proximal duodenum were used in 4x4 Latin square design to evaluate the process of mechanical maceration of sudangrass hay on the characteristics of ruminal and total tract digestion. Treatments consisted of a steam-flaked corn-based growing diet supplemented with 21 % forage (DM basis) as: i) wheat straw (Triticum aestivum, STRW), ii) sudangrass hay (Sorghum sudanense, SG), iii) macerated SG at intensity of 4,134 kPa (MAC600) and 4) macerated SG at intensity of 6,200 kPa (MAC900). All forages were ground to pass through a 3.8 cm screen before incorporation into complete mixed diets (21:79 forage to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sorghum sudanense; Feed value; Maceration; Steers; Forages. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000600001 |
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