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Leonova,Olga G; Karajan,Bella P; Ivlev,Yuri F; Ivanova,Julia L; Skarlato,Sergei O; Popenko,Vladimir I. |
We have earlier shown that the typical Didinium nasutum nucleolus is a complex convoluted branched domain, comprising a dense fibrillar component located at the periphery of the nucleolus and a granular component located in the central part. Here our main interest was to study quantitatively the spatial distribution of nucleolar chromatin structures in these convoluted nucleoli. There are no "classical" fibrillar centers in D.nasutum nucleoli. The spatial distribution of nucleolar chromatin bodies, which play the role of nucleolar organizers in the macronucleus of D.nasutum, was studied using 3D reconstructions based on serial ultrathin sections. The relative number of nucleolar chromatin bodies was determined in macronuclei of recently fed, starved... |
Tipo: Journal article |
Palavras-chave: 3D reconstruction; Ciliates; Electron microscopy; Macronucleus; Nucleoli. |
Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602013000100010 |
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