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Li,Shuhua; Qian,Xiaohua; Yuan,Zhengan; Sun,Xiaodong; Li,Chongshan; Tang,Xian; Yang,Yanji; Gong,Xiangzhen; Cao,Guangwen. |
Purpose:The purpose of this study was to identify measles virus in Shanghai in 2012 and study the genotype trend of measles virus epidemic strains during 2000–2012.Methods:Nose and throat swab specimens were collected from 34 suspected measles cases in Shanghai. Measles virus was isolated using Vero-SLAM cells (African green monkey kidney cells/lymphoid signal activating factor-transfected African green monkey kidney cells). The 450 bp of C terminus of the N gene and the entire hemagglutinin gene sequence was amplified using RT-PCR. Phylogenetic analysis was performed by comparing the seven measles strains in Shanghai with the reference strains for H1a, H1b and D8 genotypes, as well as the Chinese measles virus vaccine strain.Results:Seven measles viruses... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: D8 genotype; Importation; Measles virus; Molecular epidemiologya. |
Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000600581 |
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Deng,Xiuying; Hu,Ying; Lu,Peishan; Zhou,Ming-hao; Guo,Hongxiong. |
ABSTRACT Here we reported the outbreak of measles cases caused by the genotype D8 measles virus for the first time in Jiangsu province in China, which was possibly imported by a foreign student from Laos. Throat swab specimens were collected, and used to isolate virus. 634-bp fragment of the N gene and 1854-bp fragment of H gene were amplified by reverse transcription-PCR and sequenced, respectively. Phylogenetic results indicated that they belonged to genotype D8 measles virus. Further epidemiology investigation showed that the adults with D8 measles virus infection did not receive measles vaccine before having measles. In China, almost all D8 genotype MeV only infected those population without receiving measles vaccine immunization. Therefore, it is... |
Tipo: Info:eu-repo/semantics/report |
Palavras-chave: Measles virus; Imported D8 genotype; Outbreak. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000100066 |
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Santos,P.R.; Azevedo,M.L.B.; Borges,M.B.J.; Freire,M.S.; Nascimento,J.P.; Moraes,M.T.B.. |
Measles virus is a highly contagious agent which causes a major health problem in developing countries. The viral genomic RNA is single-stranded, nonsegmented and of negative polarity. Many live attenuated vaccines for measles virus have been developed using either the prototype Edmonston strain or other locally isolated measles strains. Despite the diverse geographic origins of the vaccine viruses and the different attenuation methods used, there was remarkable sequence similarity of H, F and N genes among all vaccine strains. CAM-70 is a Japanese measles attenuated vaccine strain widely used in Brazilian children and produced by Bio-Manguinhos since 1982. Previous studies have characterized this vaccine biologically and genomically. Nevertheless, only... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Measles virus; Vaccine; Nucleotide sequence; RNA sequencing. |
Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003001100005 |
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