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| Mimica,M.J.; Berezin,E.N.; Carvalho,R.L.B.; Mimica,I.M.; Mimica,L.M.J.; Sáfadi,M.A.P.; Schneider,E.; Caiaffa-Filho,H.H.. |
| We evaluated the performance of several methods for the detection of methicillin resistance in Staphylococcus aureus using 101 clinical S. aureus isolates from pediatric patients in a tertiary hospital in Brazil; 50 isolates were mecA-positive and 51 were mecA-negative. The Etest and oxacillin agar screening plates were 100% sensitive and specific for mecA presence. Oxacillin and cefoxitin disks gave sensitivities of 96 and 92%, respectively, and 98% specificity. Alterations of CLSI cefoxitin breakpoints increased sensitivity to 98%, without decreasing specificity. Our results highlight the importance of a continuing evaluation of the recommended microbiological methods by different laboratories and in different settings. If necessary, laboratories should... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MRSA; Disc diffusion; Cefoxitin; MecA. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702007000400009 |
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| Calazans-Silva,Amanda C.; Medeiros,Pedro T.C.; Araujo,Dayanne M.; Carvalho,Bruno O.; Coelho,Irene S.; Coelho,Shana M.O.; Souza,Miliane M.S.. |
| Oxacillin/methicillin-resistance is related to the mecA and its regulatory genes mecR1 and mecI. Its origin is still unknown, although evidences support that it is related to CNS, once mecA and a homologue gene, pbpD, were both detected in Staphylococcus sciuri species group. The present work evaluated 210 samples of skin and ear swabs from rodents and 60 nasal swabs from equines of Army Biologic Institute, Rio de Janeiro. Pheno- and genotypic characterization provided 59.52% (25/42) and 78.57% (11/14) S. lentus and S. sciuri, respectively. It was observed that although all S. sciuri isolates tested positive for pbpD, there was no correlation with oxacillin-resistance. On the other hand, isolates tested positive for mecA gene also presented phenotypic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Staphylococcus sciuri; MecA; PbpD; Antimicrobial resistance. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200038 |
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| Alcaráz,Lucía E.; Satorres,Sara E.; Lucero,Rodolfo M.; Centorbi,Olga N. Puig de. |
| Ninety-two coagulase negative staphylococci (CNS) (forty-five of clinical origin and forty-seven of environmental origin), collected in a hospital in San Luis, Argentina, from March to June, 1999, were identified to species level by the ID 32 Staph and API Staph System (bioMérieux). Slime production was investigated by the quantitative and qualitative methods. Oxacillin susceptibility was determined by the disk diffusion test (1 µg), the agar dilution method (0.125 to 4 mg/ml) and agar screen (6 µg/ml). The presence of mecA gene was investigated by PCR. The clinical CNS species most commonly isolated were S. epidermidis, S. haemolyticus, S. hominis and S. saprophyticus. The frequency of slime production by clinical and environmental isolates was similar... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coagulase-negative staphylococci; Slime; MecA; Oxacillin-R. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000100010 |
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| Martini,Rosiéli; Hörner,Rosmari; Graichen,Daniel Ângelo Sganzerla. |
| Abstract In recent years, several studies have described the clinical impact of bacterial infection associated with transfusion of platelet concentrates (PCs). Among the blood components, PCs are responsible for the highest rates of bacterial contamination as well as septic transfusion reactions. We assessed antimicrobial susceptibility profile, resistance to methicillin (MRCoNS), and resistance to macrolides, lincosamides and streptogramins of group B (MLSB) of 16 coagulase-negative staphylococci (CoNS) isolates from an investigation in 691 PCs bags. We then compared conventional and automated phenotypic methods, disc diffusion test (DD) and VITEK(r) 2, respectively as well as phenotypic and genotypic methods (Polymerase Chain Reaction - PCR). All CoNS... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacterial resistance; Staphylococcus sp; Platelet concentrates; MecA; Erm.. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100609 |
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