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Viability of Human Gingival Fibroblast (FGH) Treated with Ethanolic "Aroeira" Extract (Myracrodruon urundeuva Allemão) BABT
Machado,Alessandra Cury; Sartori,Patrícia Fernandes; Damante,Carla Andreotti; Dokkedal,Anne Lígia; Oliveira,Rodrigo Cardoso de.
ABSTRACT The aim of this study was to evaluate the effect of ethanolic "aroeira" (Myracrodruon urundeuva) extract on the viability of human gingival fibroblast. For this, fibroblasts (2x103 cells/well) were plated in a 96-well plate and incubated for 24 h; the medium (Eagle's medium modified by Dulbecco - DMEM) supplemented with 10% fetal bovine serum was replaced by DMEM with different ethanolic extract concentration (0, 0.1, 1, 10, 100, and 1000μg / mL). The fibroblast viability was analyzed after 48,72, and 96 h by the neutral red capture test and violet crystal. The "aroeira" extract, at high concentrations (100 and 1000 µg/mL) caused decrease in both cellular viability tests (p<0.05). However, dilutions between 0.1 and 10 µg/mL did not affect the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal Plant; Fibroblast; Myracrodruon urundeuva; "aroeira"; "aroeira-do-sertão".
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100301
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Vasodilator activity of extracts of field Alpinia purpurata (Vieill) K: Schum and A. zerumbet (Pers.) Burtt et Smith cultured in vitro BJPS
Victório,Cristiane Pimentel; Kuster,Ricardo Machado; Moura,Roberto Soares de; Lage,Celso Luiz Salgueiro.
Nowadays, the high blood pressure is one of the main causes of death and cardiovascular diseases. Vasodilator drugs are frequently used to treat arterial hypertension. Experiments were undertaken to determine whether hydroalcoholic extracts obtained from leaves of field-grown Alpinia purpurata and A. zerumbet cultured in vitro under different plant growth regulators induce a vasodilator effect on Wistar rat mesenteric vascular bed pre-contracted with norepinephrine. Plant extracts were able to induce a long-lasting endothelium-dependent vasodilation. Efficiency on activity of A. purpurata reached 87% at concentration of 60 μg. The extract of A. zerumbet maintained in medium containing IAA, induced the relaxation (17.4%) at 90 μg, as compared to the control...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alpinia purpurata (Vieill) K. Schum./pharmacognosy; A. zerumbet (Pers.) Burtt et Smith./pharmacognosy; Natural vasodilators/experimental studies; Plant extracts/vasodilator action; Plant tissue/culture; Endothelium-dependent; Flavonoids; Medicinal Plant; Plant growth/regulators; Zingiberaceae.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000300017
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