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Evaluation of antinociceptive and anti-inflammatory activities of the topical preparation of Cipura paludosa (Iridaceae) Acta Amazonica
Silva Neto,José Antônio Paixão da; Menezes,Larissa Dias; Gomes,Gúbio Oliveira; Cunha,Elise Marques Freire; Azevedo,Mariângela Soares de; Ferreira,Vania Moraes; Silva,Mônica Valero da.
Alho do mato (Cipura paludosa, Iridaceae) is a medicinal plant found in the Amazon rain forest, North of Brazil. It has been used to treat algic, inflammatory and infectious processes. The aim of this study was to evaluate the anti-inflammatory and antinociceptive action of the crude Cipura paludosa ethanolic extract at concentrations ranging between 2.0 and 4.0% in Oil and Water cream formulations for topical use. The physical-chemical stability of the formulations was monitored over a six-month period with the use of accelerated stability tests. In order to evaluate the anti-inflammatory and antinociceptive activities, we used a paw edema test induced by carrageenan and a formalin test, respectively. The paw edema test showed that there was a statistical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oil and Water Cream; Medicinal plant; Paw edema test; Formalin test.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672014000200012
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Valuation of the Aroeira (Myracrodruon urundeuva Allemão): perspectives on conservation Acta Botanica
Monteiro,Julio Marcelino; Araújo,Elcida de Lima; Amorim,Elba Lúcia Cavalcanti; Albuquerque,Ulysses Paulino de.
Assigning an economic value to a given environmental asset has helped conservation proposals, and valuing these assets at levels comparable to market rates facilitates the establishment of environmental policies. Thus, this research aimed to assess the value of the species Myracrodruon urundeuva Allemão, using the method of contingent valuation to determine the maximum willingness to pay of frequent visitors to the Caruaru Fair, located in northeastern Brazil. Our results show that most interviewees agreed to participate in proposals to support species conservation, although the average willingness to pay (WTP) was relatively low compared to other surveys conducted in Brazil. However, a significant number of the interviewees had exceptionally low monthly...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Contingent valuation; Caatinga; Medicinal plant.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062012000100014
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Chemical diversity of accessions of the in vivo germplasm bank of Varronia curassavica (Jacq.) Agronomy
Queiroz, Túlio Barroso; Fonseca, Francine Souza Alves da; Mendes, Aretusa Daniela Resende; Azevedo, Alcinei Mistico; Martins, Ernane Ronie.
Varronia curassavica (Jacq.) is a shrub and perennial plant native to Brazil that has therapeutic, anti-inflammatory and cicatrizing properties. The objective of this work was to study the chemical diversity of the native populations of this species maintained in the in vivo germplasm bank from Montes Claros, Minas Gerais, Brazil. The essential oils of 10 accessions analyzed in triplicate were obtained by hydrodistillation. Fifty-five compounds were detected, 46 of which were identified by GC-MS analysis. Essential oil yield ranged from 0.1 (VC-9) to 1.22% (VC-3) among the studied accessions. Three groups were formed by cluster analysis. Group A presented lower relative abundance of the compounds α-humulene (1.4%) and β-caryophyllene (8.5%). Conversely, in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Essential oil; Medicinal plant; Chemotype; Α-humulene; Β-caryophyllene.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42726
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Phosphorus nutrition in the growth of Bauhinia forficata L. seedlings - DOI: 10.4025/actasciagron.v30i5.787 Agronomy
Brondani, Gilvano Ebling; Esalq/USP; Silva, Ariadne Josiane Castoldi; UFPR; Araujo, Marla Alessandra de; UFPR; Grossi, Fernando; UFPR; Wendling, Ivar; Embrapa; Carpanezzi, Antonio Aparecido; Embrapa.
One of the greatest problems in the elaboration of forestation programs using native species is the lack of knowledge about seedling production. This study aimed to evaluate the growth of Bauhinia forficate seedlings submitted to phosphorus levels. Eleven-day-old seedlings were transplanted to small tubettes (110 cm3) with pinus bark and vermiculite substratum base. The experiment was conducted in an entirely randomized delineation, with six P treatments: T0-control (substratum without P addition), T1-50, T2-100, T3-150, T4-200 and T5-250 mg dm-3, with five replications and 20 seedlings per replication. After 60 days, the seedlings presented a 98.2% survival rate and good root formation. The height varied in function of P levels, with maximum production up...
Palavras-chave: 5.01.03.00-8 Fitotecnia produção de mudas; Adubação; Doses de P; Espécie nativa da América do Sul; Fabaceae; Planta medicinal Produção de Mudas seedling production; Fertilization; P levels; South America native species; Fabaceae; Medicinal plant.
Ano: 2008 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/787
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Teor e composição química do óleo essencial de Melissa officinalis L. in vitro sob influência do meio de cultura - DOI: 10.4025/actasciagron.v31i2.765 Agronomy
Reis, Érika Soares; IFMG; Pinto, José Eduardo Brasil Pereira; UFLA; Rosado, Luciana Domiciano Silva; UFLA; Corrêa, Ricardo Monteiro; IFMG.
Com o objetivo de avaliar o efeito do meio de cultura no teor e composição química do óleo essencial de Melissa officinalis, foi realizado o presente trabalho, que avaliaram três concentrações do meio de cultura MS. O experimento foi implantado em delineamento inteiramente casualizado, com sete repetições, e cada repetição foi representada por uma amostra de 20 g de plântulas frescas. A extração do óleo foi realizada em aparelho de Clevenger modificado com duração de 1h e 30 min. e a análise química foi realizada por cromatografia gasosa. Observou-se que o teor e a composição química do óleo essencial de M. officinalis foram influenciados pela concentração de sais do meio MS. As plântulas que se desenvolveram nos meios MS e MS/4 apresentaram maior teor de...
Palavras-chave: 5.01.00.00-9 Agronomia Melissa officinalis; Planta medicinal; Óleo essencial; Cultura de tecidos Fitotecnia Melissa officinalis; Medicinal plant; Essential oil; Tissue culture.
Ano: 2009 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/765
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Genetic diversity of Lippia rotundifolia Cham. in Minas Gerais, Brazil Biological Sciences
Meira, Messulan Rodrigues; Martins, Ernane Ronie; Resende, Luciane Vilela.
Lippia rotundifolia is an aromatic species, endemic from Campos rupestres and isolated by mountains. We aimed with the research to study the genetic variability of chá-de-pedestre (Lippia rotundifolia Cham.) with natural occurrence in ten places of the State of Minas Gerais, from molecular markers of ISSR type. The places of collection were: Parque Estadual de Serra Nova; Parque Estadual Veredas do Peruaçu; Abóboras community; Gigante community; edge of Rio do Peixe; environmental preservation area of Olhos d´água; private property in Joaquim Felício; Parque Estadual do Rio Preto; São Gonçalo do Rio das Pedras and brook of Rio Tigre. After the establishment of the extraction and amplification of DNA fragments with primers ISSR, an array of genetic distance...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.02.03.00-4 pedestrian tea; Genetic variability; Medicinal plant; Conservation Genética Vegetal.
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/42754
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Polysaccharide extract of Mimosa tenuiflora stem barks stimulates acute inflammatory response via nitric oxide Biological Sciences
Silva-Leite, Kaira Emanuella Sales da; Queiroz, Cinthia Silva; Madeira, Juliana da Costa; Soares, Pedro Marcos Gomes; Pereira, Maria Gonçalves; Assreuy, Ana Maria Sampaio.
Mimosa tenuiflora (Mimosaceae) or “jurema-preta” is well distributed in the northeast Brazil, being popularly used to treat skin lesions, burns and inflammation. The healing effect of the alcoholic extract prepared with its barks corroborates the popular use. This study aimed to evaluate the inflammatory response of polysaccharides extracted from M. tenuiflora barks (EP-Mt) by methanol/NaOH and ethanol precipitation. Inflammatory activity was assessed in rat models of acute inflammation (paw edema and peritonitis), by the following parameters: edema, vascular permeability, leukocyte migration, myeloperoxidase activity and pharmacological modulation of nitric oxide and prostaglandins. EP-Mt presented 3.8% yield, 41% carbohydrate and 0.34% protein. EP-Mt...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.00.00.00-6 e 2.10.00.00-0 Mimosaceae; Medicinal plant; Carbohydrate; Inflammation 2.10.06.00-8.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/32234
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Active phloem sap collection by Trigona spinipes (Fabricius) (Hymenoptera, Apidae) in Artemisia annua Linn (Asteraceae) Biological Sciences
Malagodi-Braga, Kátia Sampaio; Moriconi, Waldemore; Queiroga, Joel Leandro de; Urchei, Mário Artemio; Pazianotto, Ricardo Antônio Almeida; Roncon, Kenny.
Trigona spinipes Fabricius (Apidae: Meliponini), is a common stingless bee widely seen in urban and rural areas in Brazil, popularly known as irapuá, arapuá or bee-dog. Although these bees are considered pollinators of some cultivated plants, they are better known for the damage they cause in different crops. During experimental agroecological cultivation of Artemisia (Artemisia annua Linn, Asteraceae), in Jaguariúna (SP, Brazil), stingless bees Trigona spinipes (Fabricius) were observed sucking phloem sap directly from the plant, a phenomenon not yet described in scientific literature. This study aimed to register and describe the phloem sap-sucking behavior performed by T. spinipes for the first time, as well as to assess the potential impact of this...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plant; Insect behavior; Plant injury; Stingless bee Farming..
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/47548
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Antibacterial effects of Thymus algeriensis extracts on some pathogenic bacteria Biological Sciences
Messaoudi, Mohammed; Benreguieg, Mokhtar; Merah, Maroua; Messaoudi, Zakarya Ayoub.
Despite the presence of many antibiotics, bacterial resistance is growing steadily and some of these antibiotics have become ineffective, which poses a major challenge to the health sector. In this context, this work has demonstrated, in vitro, the inhibitory action of the bacterial growth resulting from methanolic and ethanolic extracts of Thymus algeriensis Boiss. & Reut., a medicinal plant species harvested from the Algerian South-west area, as well as the determination of the phenolic content of those crude extracts. The methanolic extract of Thymus algeriensis showed a significant antibacterial effect with 16.5 and 19 mm against Pseudomonas aeruginosa and Staphylococcus aureus, respectively. Klebsiella pneumoniae was not inhibited by both...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antibacterial effect; Extracts; Medicinal plant; Phenolic content; Thymus algeriensis..
Ano: 2019 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/48548
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Leaf anatomy of Cordiera sessilis (Vell.) Kuntze (RUBIACEAE) Biological Sciences
Teixeira, Thaiz Rodrigues; Pádua, Marlúcia Souza; Castro, Ana Hortência Fonsêca.
A study on the leaf anatomy of Cordiera sessilis (Rubiaceae), a native medicinal shrub from Brazilian Cerrado was carried out to contribute for species identification and provide support for future biochemical studies and medicinal application. Leaves were collected, fixed and processed by usual techniques, and studied by light and electron microscopy. Quantitative analyzes of stomata and trichomes were performed. The leaves showed typical characteristics of Rubiaceae family, like hypostomatic leaves, paracytic stomata, dorsiventral mesophyll and collateral bundles. Two types of vascular patterns were identified in the petiole: in distal part, the vascular system is arranged cylindrically surrounded by sclerenchyma sheath and in proximal part, the vascular...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.03.00.00-0 e 2.03.02.03-7 Marmelinho; Plant anatomy; Histochemical tests; Cerrado; Medicinal plant Botânica “Marmelinho”; Plant anatomy; Histochemical tests; Cerrado; Medicinal plant.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/29902
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In vitro germination, callus induction and phenolic compounds contents from Pyrostegia venusta (Ker Gawl.) Miers Biological Sciences
Braga, Karina de Queiroz; Coimbra, Mairon César; Castro, Ana Hortência Fonsêca.
A protocol for the in vitro germination and callus induction in Pyrostegia venusta, a medicinal plant species from the Brazilian savannah (Cerrado) is provided. The establishment of cultures of P. venusta was originally obtained from seeds germinated in vitro and induced callus directly from leaf explants of seedlings. Seeds were germinated on MS and WPM media containing 100 and 50% of salt concentration, supplemented with 30 g L-1 sucrose. Callus induction consisted of the inoculation of leaf segments on MS medium plus 2,4-D or BAP in the presence or absence of light. The germination percentage averaged 85% and the aerial parts and roots of seedlings obtained in WPM with 50 and 100% of salt concentration showed elevated contents of total phenolic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.00.00.00-6; 2.03.00.00-0 seeds; Auxin; Cytokinin; Medicinal plant; Flavonoids 2.03.03.00-9.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/26067
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Moisture content on some engineering properties of celery (Apium Graveolens L) seeds CIGR Journal
Mirzabe, Amir Hossein; Taheri, Mojtaba; Pouyesh, Alireza; Bahrami Bavani, Neda.
The study was conducted to investigate some physical properties of celery seed at various moisture levels.  The average length, width, thickness and 1000-seeds mass were 0.571, 0.429, 0.295 mm and 0.792 g, respectively, at moisture content of 5.24% (d.b).  Length, width and thickness distributions of the seeds were modeled using Generalized Extreme Value, lognormal and Weibull distributions.  Results showed that to model length of the seeds, lognormal distribution had the best performance while to model the width of the seeds Weibull distribution had the best performance.  True density has increase from 892.02 to 931.42 kg/m3 when the moisture content increased from 5.24% to 20.25% (d.b).  The angle of static friction increased from 28.45 to 39.66°, 25.15...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Image processing technique; Dimensional properties; Modeling; Gravimetrical properties; Medicinal plant.
Ano: 2016 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/3559
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In vivo assessment of cyto/genotoxic, antigenotoxic and antifungal potential of Costus spiralis (Jacq.) Roscoe leaves and stems Anais da ABC (AABC)
SOUSA,WENDEL C. DE; T.S. PAZ,ANDRESSA; ROCHA,JAMIRA D.; CONCEIÇÃO,EDEMILSON C. DA; ALMEIDA,LUCIANE M. DE; CHEN,LEE C.; BORGES,LEONARDO L.; BAILÃO,ELISA F.L.C..
ABSTRACT Costus spiralis is a Brazilian native plant used in popular medicine, but the safety of this therapeutic use needs investigation. So, the aim of this study was to evaluate the cytogenotoxic and antigenotoxic effects of C. spiralis leaves or stems aqueous extracts on Allium cepa root cells. Moreover, a phytochemical screening and an antioxidant and antifungal activities evaluation were performed. C. spiralis aqueous extracts presented cytotoxicity, but no mutagenicity was observed. When the antigenotoxicity was evaluated, C. spiralis leaves aqueous extract presented preventive and modulatory effects on A. cepa root cells, reducing the sodium azide cytogenotoxic effects. In contrast, C. spiralis stems aqueous extract enhanced the sodium azide...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allium cepa test; Antioxidant; Medicinal plant; Secondary metabolites.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000401565
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Plant growth, nutrients and potentially toxic elements in leaves of yerba mate clones in response to phosphorus in acid soils Anais da ABC (AABC)
BARBOSA,JULIERME Z.; MOTTA,ANTONIO C. V.; CONSALTER,RANGEL; POGGERE,GIOVANA C.; SANTIN,DELMAR; WENDLING,IVAR.
ABSTRACT Native to subtropical region of South America, yerba mate is responsive to P under some conditions, but the degree of influence of genetic and soil on the growth and composition of the leaf is unknown. The aim of study was to evaluate plant growth, nutrients and potentially toxic elements in leaves of yerba mate clones in response to P application in acid soils. In greenhouse condition, two yerba mate clone seedlings were grown (210 days) in pots, each clone in a completely randomized design in factorial scheme (with and without P; four acid soils). The elemental composition of leaves and the growth of plants were determined. Phosphorus promoted plant growth, but this was not accompanied by increased P in leaf tissue in all conditions tested. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ilex paraguariensis St.Hil; Medicinal plant; Metal accumulation; Soil fertility; Trace elements..
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000100557
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Evaluation of wound healing and antimicrobial properties of aqueous extract from Bowdichia virgilioides stem barks in mice Anais da ABC (AABC)
AGRA,ISABELA K.R.; PIRES,LUANA L.S.; CARVALHO,PAULO S.M.; SILVA-FILHO,EURÍPEDES A.; SMANIOTTO,SALETE; BARRETO,EMILIANO.
The decoction of the stem barks from Bowdichia virgilioides KUNTH is a folk remedy used to treat inflammatory disorders in Latin American and Brazil. In the present study, the wound healing activity of aqueous extract of the stem bark from B. virgilioides, called AEBv, was evaluated by the rate of healing by wound contraction and period of epithelization at different days post-wound using the wound excisional model. On day 9, the AEBv-treated animals exhibited significative reduction in the wound area when compared with controls. In wound infected with S. aureus, the AEBv significantly improved the wound contraction when compared to the saline-treated mice. The histological analysis showed that AEBv induced a collagen deposition, increase in the fibroblast...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Wound healing; Antimicrobial effect; Bowdichia virgilioides; Medicinal plant.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000300945
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Assessment of the cytotoxic, genotoxic, and antigenotoxic activities ofCeltis iguanaea (Jacq.) in mice Anais da ABC (AABC)
BORGES,FLAVIO F.V.; MACHADO,THIAGO C.; CUNHA,KÊNYA S.; PEREIRA,KARLA C.; COSTA,ELSON A.; PAULA,JOSÉ R. DE; CHEN-CHEN,LEE.
Ethnobotanical surveys of Cerrado native plants show that leaves of Celtis iguanaea (Jacq.) Sargent (Cannabaceae), popularly known in Brazil as “esporão de galo”, are used in folk medicine for body pain, asthma, cramps, poor digestion, urinary infection, kidney dysfunctions, as well as a stimulant and diuretic. This work aimed at evaluating possible C. iguanaea aqueous leaf extract (CALE) cytotoxicity, genotoxicity, and antigenotoxicity using the mouse bone marrow micronucleous test. To assess CALE genotoxicity, Swiss mice were orally treated with three different extract concentrations (100, 300, and 500 mgkg−1). To evaluate its antigenotoxicity, the same doses were used simultaneously with a single i.p. dose of mitomycin C (MMC, 4mg.kg−1). The frequencies...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytotoxicity; Genotoxicity; Mice; Medicinal plant.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000300955
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Antifungal activities of the essential oil and its fractions rich in sesquiterpenes from leaves of Casearia sylvestris Sw. Anais da ABC (AABC)
PEREIRA,FLAVIANE G.; MARQUETE,RONALDO; DOMINGOS,LEVY T.; ROCHA,MARCO E.N.; FERREIRA-PEREIRA,ANTONIO; MANSUR,ELISABETH; MOREIRA,DAVYSON L..
ABSTRACT Casearia genus (Salicaceae) is found in sub-tropical and tropical regions of the world and comprises about 160-200 species. It is a medicinal plant used in South America, also known as “guaçatonga”, “erva-de-tiú”, “cafezinho-do-mato”. In Brazil, there are about 48 species and 12 are registered in the State of Rio de Janeiro, including Casearia sylvestris Sw. There are many studies related to the chemical profile and cytotoxic activities of extracts from these plants, although few studies about the antifungal potential of the essential oil have been reported. In this work, we have studied the antifungal properties of the essential oil of C. sylvestris leaves, as well as of their fractions, against four yeasts (Saccharomyces cerevisae, Candida...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Candida sp.; Casearia genus; Essential oil; Medicinal plant; Saccharomyces cerevisae..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000602817
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Chemical diversity of the volatiles of Lippia rotundifolia Cham. (Verbenaceae) in Minas Gerais, Brazil Anais da ABC (AABC)
MEIRA,MESSULAN R.; MARTINS,ERNANE R.; FONSECA,FRANCINE S.A. DA.
Lippia rotundifolia is an aromatic species, native and endemic to rocky fields, which are isolated by small mountains. It is little known about their chemical composition. Because of that we aimed to study the chemical diversity of volatiles released from rosemary leaves (Lippia rotundifolia Cham.) coming from 11 populations of the Minas Gerais, Brazil. The material for chemical analysis was obtained from the accessions in the active germplasm bank of the Instituto de Ciências agrárias of the Universidade Federal de Minas Gerais. A total of 67 compounds were detected by HS-CG-MS. Most volatiles were detected in the São Gonçalo do Rio das Pedras accession, with 40 compounds, whereas in the Santana do Riacho accession only 5 compounds were detected....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chemical variability; Medicinal plant; Rosemary; Static headspace.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100618
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Alterations in the mitotic index of Allium cepa induced by infusions of Pluchea sagittalis submitted to three different cultivation systems Anais da ABC (AABC)
Rossato,Liana V.; Tedesco,Solange B.; Laughinghouse IV,Haywood D.; Farias,Júlia G.; Nicoloso,Fernando T..
We evaluated the antiproliferative effect of infusions from Pluchea sagittalis using the Allium cepa test. Infusions in three concentrations (2.5, 5, and 25 g dm-3) of leaves cultivated in three environments (in vitro, acclimatized growth chamber, and field) were used. Six onion bulbs were used for each of the eight treatments, and the mitotic index was obtained from 6000 cells per treatment. In conclusion, leaf infusions of P. sagittalis cultivated in the field have a high antiproliferative activity, as well as the cultivation system influences the antiproliferative potential.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antiproliferative effect; Camphorweed; Cell cycle; Medicinal plant; Mutagenicity.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000400007
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Action of Aqueous Extracts of Phyllanthus niruri L. (Euphorbiaceae) leaves on Meristematic Root Cells of Allium cepa L. Anais da ABC (AABC)
NEVES,ERASMOVLANE S.B.; FERREIRA,PAULO MICHEL PINHEIRO; LIMA,LEONARDO H.G.M.; PERON,ANA PAULA.
This study aimed to evaluate the effects of aqueous extracts of dried Phyllanthus niruri L. (stonebreaker) leaves on Allium cepa L. root meristem cells at four concentrations, 0.02 (usual concentration), 0.04, 0.06 and 0.08mg/mL and exposure times of 24 and 48 hours. For each concentration we used a group of five onion bulbs that were first embedded in distilled water and then transferred to their respective concentrations. The radicles were collected and fixed in acetic acid (3:1) for 24 hours. The slides were prepared by the crushing technique and stained with 2% acetic orcein. Cells were analyzed throughout the cell cycle, totaling 5000 for each control and exposure time. The calculated mitotic indices were subjected to the Chi-squared statistical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Medicinal plant; Stonebreaker; Cell division; Cellular aberrations; Allium cepa.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000301131
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