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COMPORTAMIENTO MEIÓTICO EN VAINILLA (V. planifolia Jacks., ORCHIDACEAE) Acta biol.Colomb.
Almejo Vázquez,Lizette I.; Iglesias Andreu,Lourdes G.; Escobedo Gracia-Medrano,Rosa Maria.
RESUMEN El comportamiento meiótico es de gran importancia para el desarrollo de programas de mejoramiento genético, especialmente en especies como la vainilla (Vanilla planifolia Jacks.), que se ven severamente afectadas por diversos factores bióticos y abióticos derivados de su estrecha base genética. Por ello, el presente trabajo se planteó con el objetivo de evaluar el comportamiento meiótico del morfotipo comercial "Mansa" del municipio de Papantla de Olarte, Veracruz, México. Para ello, se colectaron botones florales de diferentes tamaños y se fijaron en Carnoy, utilizando la técnica de la calabaza, seguido de una tinción con acetocarmín al 2% (p/v) para observar claramente todas las etapas del proceso meiótico. Se observó un comportamiento meiótico...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Citogenética; Cromosomas; Meiosis; Mejoramiento genético; Orchidaceae.
Ano: 2022 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2022000300464
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LA COMPETENCIA DEL OVOCITO: QUÉ, CÓMO Y CUÁNDO Acta biol.Colomb.
TARAZONA¹,ARIEL; LÓPEZ,ANGELA; OLIVERA-ANGEL,MARTHA.
La producción de embriones in vitro ha sido una herramienta útil para el desarrollo de técnicas como la clonación y la transgénesis, que han revolucionado los campos de la ciencia y la tecnología. La técnica básica para la maduración, fertilización y cultivo in vitro de los embriones produce, desde sus inicios, bajas tasas de desarrollo hasta el estadío de blastocisto con índices reducidos de viabilidad de los mismos. Los avances en el conocimiento de la dinámica celular durante estos procesos condujo a la hipótesis, de que la maduración, entendida como el reinicio del ciclo meiótico, la reorganización del ooplasma y la regulación de la expresión génica, juegan un papel fundamental para sustentar el desarrollo temprano de los embriones, esta capacidad de...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Complejo folicular; Maduración; Meiosis; Ooplasma.
Ano: 2010 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2010000300001
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Meiotic behavior in Panicum maximum Jacq. (Poaceae: Panicoideae: Paniceae): hybrids and their genitors - doi: 10.4025/actasciagron.v32i3.6461 Agronomy
Pessim, Cleide; UEM; Pagliarini, Maria Suely; UEM; Jank, Liana; Embrapa; Kaneshima, Alice Maria de Souza; Universidade Estadual de Maringá; Bonato, Andréa Beatriz Mendes; Universidade Estadual de Maringá.
Panicum maximum Jacq. is one of the most cultivated grasses in the world. The intraspecific hybridization breeding program underway at Embrapa Beef Cattle aims at combining several agronomic characteristics in a single plant with high seed production. Four intraspecific hybrids originated from the cross between sexual tetraploid female plants with apomictic tetraploid male plants, including the genitors, were cytologically evaluated in the meiotic process throughout conventional methodology. Hybrids and genitors showed high meiotic stability. The percentage of abnormal cells among them ranged from 6.7 to 14.2%. Abnormalities recorded were irregular chromosome segregation, chromosome stickiness, and absence of the first cytokinesis, but the latter did not...
Palavras-chave: 5.01.03.05-9 breeding program; Meiosis; Intraspecific hybrids; Panicum maximum 5.01.03.05-9 breeding program; Meiosis; Intraspecific hybrids; Panicum maximum.
Ano: 2010 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/6461
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Comportamento mitótico e meiótico de cromossomos holocêntricos &gama; irradiados de Rhynchospora pubera (Cyperaceae) Biological Sciences
Vanzela, André Luís Laforga; UEL; Colaço, Waldeciro; UFPE.
Para avaliar a ocorrência de cinetócoros difusos em Rhynchospora (Cyperaceae), cromossomos de R. pubera foram fragmentados ou fundidos por irradiação &gama;. Este material foi investigado nas gerações celulares subseqüentes, em ambas, mitose e meiose. Alterações no número e no tamanho dos cromossomos foram detectadas, mas o comportamento dos cromossomos irradiados foi normal e nenhum micronúcleo foi observado. Todos os dados apontam para a presença de cinetócoros difusos em R. pubera.
Palavras-chave: 2.00.00.00-6 Ciências Biológicas Cyperaceae; Holocentric chromosomes; Meiosis; Mitosis; Rhynchospora 2.00.00.00-6 Ciências Biológicas.
Ano: 2002 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/2364
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Botanical aspects of Heteropterys umbellata (Malpighiaceae): a cytological and palynological approach Anais da ABC (AABC)
Sousa,Saulo M.; Reis,Aryane C.; Gomes,Shaiany S.L.; Bernardo,Karina B.; Salimena,Fátima R.G.; Viccini,Lyderson F..
The genus Heteropterys is one of the major genera in Malpighiaceae. However, few cytological and palynological studies were reported. The present work described for the first time the chromosome number, heterochromatin pattern, meiotic behavior, pollen viability and palynological aspects of Heteropterys ubellata, a very spread species. One large Brazilian population was evaluated using conventional techniques for meiotic studies and acetolyse to access the pollen morphology. The species showed 2n = 20 chromosomes, normal meiotic development and viable pollens. Great blocks of heterochromatin were observed around the centromers. DAPI staining was positive for centroeric heterochroatin, hile CMA3 ark as observed just at terinal regions of one pair of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome number; Heterochromatin pattern; Heteropterys umbellata; Malpighiaceae; Meiosis; Pollen viability.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652010000400009
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Effect of concentration and exposure period to butyrolactone I on meiosis progression in bovine oocytes Arq. Bras. Med. Vet. Zootec.
Adona,P.R.; Leal,C.L.V..
The effect of concentration and exposure period of bovine oocytes to butyrolactone I (BLI) on meiotic block and in vitro maturation (IVM) kinetics was studied. In experiment 1, all oocytes were at germinal vesicle stage (GV), after 6h in culture with 0, 50 and 100µM BLI. After 12h, all oocytes cultured with 50 and 100µM BLI remained in GV. After 24h, less oocytes were in GV with 50µM (82%) than with 100µM BLI (99%, P<0.05). In experiment 2, after 6h IVM, 93% of control oocytes (IVM only) were in GV, while treated oocytes (100µM BLI for 6, 12 or 24h prior to IVM) showed less oocytes in GV with increased exposure period to BLI prior to IVM (83 and 73%, for 6h and 12h, P<0.05). For a 24h inhibition, GV rates were similar to 12h (70%, P>0.05). After...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Meiosis; Oocyte; Butyrolactone I; Kinetics; Nuclear maturation.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352006000300011
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Synaptonemal complexes and XY behavior in two species of argentinian armadillos: Chaetophractus villosus and Dasypus hybridus (Xenarthra, Dasypodidae) Biocell
Sciurano,R. B.; Merani,M.S.; Bustos,Jimena; Solari,A.J..
Spermatocytes from the two armadillo species, C. villosus and D. hybridus were studied in microspreads for synaptonemal complexes (SCs) and in thin sections for electron microscopy (EM). The complete SC karyotype generally agrees with previous reports on mitotic chromosomes, except for the sex chromosomes. The X chromosome is submetacentric in both species and the Y is the shortest one in C. villosus and the second shortest in D. hybridus, and an extremely acrocentric one. A SC is formed along the total length of the Y chromosome, and this SC persists along all the pachytene substages. A single ecombination nodule (RN) is located in the region of the SC nearest to the attachment to the nuclear envelope. The lateral element (LE) of the X axis in the SC...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xenarthra; XY chromosomes; Meiosis; Synaptonemal complexes.
Ano: 2006 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452006000100009
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A male-sterile mutation in soybean (Glycine max) affecting chromosome arrangement in metaphase plate and cytokinesis Biocell
Pires Bione,Nilton Cesar; Suely Pagliarini,Maria; Alves de Almeida,Leones.
A spontaneous male-sterile, female-fertile mutation affecting bivalent arrangement at the metaphase plate and cytokinesis was detected in line BR98-197 of the soybean breeding program developed by Embrapa - National Soybean Research Centre. Untill diakinesis, meiosis was normal with chromosome pairing as bivalents. From this phase, in several meiocytes, bivalents were not able to organize a single metaphase plate and remained scattered in the cytoplasm in a few or several groups. In these meiocytes, chromosomes segregated in both divisions giving rise to several micronuclei. However, the main cause of male sterility was the absence of cytokinesis after telophase II. Instead of the typical tetrads of microspores, four nucleate coenocytic microspores were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Meiosis; Metaphase plate; Cytokinesis; Male sterility.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000200004
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Microsporogenesis in tetraploid accessions of Brachiaria nigropedata (Ficalho &amp; Hiern) Stapf (Gramineae) Biocell
Sayuri Utsunomiya,Karina; Suely Paglirini,Maria; Borges do Valle,Cacilda.
The genus Brachiaria (Trin.) Griseb. has achieved considerable importance to cattle production systems, as a result of the good production and adaptation of a few cultivars to poor and acid soils of the Brazilian savannas. Many of its species and accessions are polyploid and apomictic, which limits direct hybridization. To assist the breeding program, cytogenetic characterization has been undertaken on the accessions of Brachiaria collection at the Embrapa Beef Cattle Research Center. In this study, chromosome number and meiotic behavior are reported for the Brachiaria nigropedata (Ficalho &amp; Hiern) Stapf collection. The 20 available accessions are tetraploid (2n = 4x = 36). Chromosomes paired preferentially as bivalents, but quadrivalents were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachiaria nigropedata; Chromosome number; Meiosis; Polyploidy; Apomixis; Grasses.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000300007
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Chromosome number and microsporogenesis of two accessions of Brachiaria dura Stapf (Poaceae) Biota Neotropica
Risso-Pascotto,Claudiceia; Pagliarini,Maria Suely; Valle,Cacilda Borges do.
The two accessions of B. dura analyzed (DU01 and DU02) are hexaploid (2n = 6x = 54), derived from x = 9. Meiotic abnormalities, such as precocious chromosome migration to the poles, laggards and micronuclei, were recorded in low frequency in both accessions. The few multivalent chromosome association at diakinesis and meiotic stability suggested that hexaploidy probably resulted from chromosome doubling. In DU02, chromosome transfer (cytomixis) among meiocytes, involving part or the entire genome was observed. The implication of these findings for the Brachiaria breeding is discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachiaria dura; Meiosis; Chromosome number; Cytomixis.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032009000200024
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Mecanismos citológicos involucrados en la producción de gametos masculinos no reducidos en individuos diploides de Turnera sidoides subsp. carnea (Passifloraceae) Boletín de la Sociedad
Kovalsky,Ivana Evelin; Fernández,Aveliano; Solís Neffa,Viviana G.
Turnera sidoides (x = 7) es un complejo autopoliploide de hierbas alógamas perennes que incluye cinco subespecies, con niveles de ploidía desde diploide (2n = 2x = 14) hasta octoploide (2n = 8x = 56). Estudios previos aportaron evidencias de la producción de gametos masculinos no reducidos (2n y 4n) en poblaciones diploides del complejo, sugiriendo que los poliploides de T. sidoides habrían surgido por poliploidización sexual. Sin embargo, hasta el momento se desconocen los mecanismos citológicos involucrados en la producción de dichos gametos. Por lo tanto, en este trabajo se analiza el comportamiento meiótico y la viabilidad del polen de individuos productores de gametos no reducidos de una población diploide de T. sidoides subsp. carnea. Los resultados...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Turnera sidoides; Poliploidía; Gametos no reducidos; Meiosis; Viabilidad del polen.
Ano: 2014 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722014000200009
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Estudios citogenéticos en híbridos triploides y tetraploides de Turnera krapovickasii, T. scabra y T. subulata (Passifloraceae, Turneroideae) Boletín de la Sociedad
A fin de investigar las relaciones genómicas entre especies de la serie Turnera (x= 5), se lleva a cabo un programa de cruzamientos controlados y se obtuvieron numerosos híbridos. Entre las especies con flores amarillas de la serie Turnera, T. krapovickasii, T. scabra y T. subulata poseen citotipos diploide y autotetraploide. Hasta el momento se desconocen el grado de aislamiento reproductivo entre diploides y tetraploides y el grado de afinidad genómica de estas especies a nivel tetraploide. En este trabajo se presentan los resultados del análisis citogenético de los híbridos intraespecíficos triploides (2n=3x=15) de T. krapovickasii y T. scabra y los híbridos tetraploides (2n=4x=20) T. krapovickasii × T. scabra y T. subulata × T. scabra. En los híbridos...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Turnera; Meiosis; Relaciones genómicas; Triploides; Tetraploides.
Ano: 2017 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722017000400008
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Meiotic behavior during microsporogenesis of Alchornea triplinervia (Sprengel) Müller Argoviensis Ciência Rural
Godoy,Sara Mataroli de; Pereira,Andréia Rodrigues Alonso; Romagnolo,Mariza Barion; Risso-Pascotto,Claudicéia.
The Alchornea triplinervia specie belongs to the Euphorbiaceae family, one of the main families of the Brazilian flora. In order to contribute to a better understanding of the specie, a counting of chromosome number and the microsporogenesis analysis of A. triplinervia were done. The inflorescences were collected in the municipalities of Paranavaí and Diamante do Norte, State of Paraná, Brazil, and the slides were prepared by squashing technique and staining with 1% acetic carmine. The analysis were performed using an optical microscope and showed a chromosome number for the specie equal to 2n=8x=72. Irregularities in the chromosome segregation process were the main meiotic abnormalities, presenting typical polyploid behavior. Other irregularities were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Euphorbiaceae; Chromosome number; 2n gametes; Meiosis; Polyploid.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782012000600012
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Chromosome stickiness during meiotic behavior analysis of Passiflora serrato-digitata L. (PassifloraCEAE) Ciência Rural
Kiihl,Paulo Roberto Peres; Pereira,Andréia Rodrigues Alonso; Godoy,Sara Mataroli de; Stenzel,Neusa Maria Colauto; Risso-Pascotto,Claudicéia.
Almost 90% of species of the genus Passiflora are native to the American continent, with high commercial value due to the fact that some species are used for human food while others have ornamental and medical qualities. Passiflora serrato-digitata is one of the species that integrates the Paraná Agronomic Institute germoplasm bank at its experimental base in Londrina, PR, Brazil. Collected flower buds were fixed in ethanol/acetic acid (3:1 v/v) for 24h, transferred to 70% alcohol and stored under refrigeration. Slides were prepared by the squashing technique and stained with 1.0% propionic carmine; they were analyzed under an optic microscope. Irregularities in the chromosome segregation process of P. serrato-digitata have been verified by meiotic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsporogenesis; Meiosis; 2n gametes.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000600016
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Microsporogénesis de tres especies del subgénero Passiflora (Passiflora, Passifloraceae) de Paraguay Darwiniana
Pereira Sühsner,Claudia; Honfi,Ana I; Ferrucci,María S; Deginani,Norma.
El presente trabajo tuvo por objeto caracterizar cariológicamente las especies del género Passiflora L., subgénero Passiflora, del Distrito Félix Pérez Cardozo, Guairá, Paraguay. Por primera vez, se da a conocer el número cromosómico en meiosis 2n = 9II para P. alata Curtis y P. caerulea L. También, se confirma el número cromosómico de 2n = 9II para P. edulis Sims. El comportamiento meiótico en todas las especies estudiadas fue normal, con segregación regular de los cromosomas. La asociación cromosómica frecuentemente encontrada en diacinesis y metafase I fue de bivalentes. Las pocas irregularidades meióticas observadas consistieron en cromosomas rezagados en anafase I y fases asincrónicas en meiosis II, sin embargo, la viabilidad de polen es alta, entre...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Meiosis; Passiflora; Viabilidad del polen.
Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0011-67932016000100004
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Estudios cromosómicos en especies de Asplenium (Aspleniaceae) de la Argentina Darwiniana
Guillén,Roque H.; Daviña,Julio R..
Documentamos los números cromosómicos meióticos y somáticos de 6 especies del género Asplenium. Se presentan por primera vez los números cromosómicos de A. ulbrichtii var. serrato-dentatum, 2n=2x=72, y A. triquetrum, 2n=4x=144. Reconfirmamos los números cromosómicos de A. auritum, n=72; A. claussenii, 2n=2x=72; A. formosum, n=36; y A. serratum, 2n=8x=288.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Argentina; Asplenium; Números cromosómicos; Helechos; Meiosis; Pteridophyta.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0011-67932005000100004
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Abnormal spindle orientation during microsporogenesis in an interspecific Brachiaria (Gramineae) hybrid Genet. Mol. Biol.
Mendes-Bonato,Andréa Beatriz; Pagliarini,Maria Suely; Valle,Cacilda Borges do.
This paper reports a case of abnormal spindle orientation during microsporogenesis in an interspecific hybrid of the tropical grass Brachiaria. In the affected plant, prophase I was normal. In metaphase I, bivalents were regularly co-oriented but distantly positioned and spread over the equatorial plate. In anaphase I, chromosomes failed to converge into focused poles due to parallel spindle fibers. As a consequence, in telophase I, an elongated nucleus or several micronuclei were observed in each pole. In the second division, the behavior was the same, leading to polyads with several micronuclei. A total of 40% of meiotic products were affected. The use of this hybrid in production systems needing good-quality seeds is discussed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brachiaria; Interspecific hybrid; Meiosis; Spindle abnormality; Tropical grass.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100023
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Chromosomes of Theridiidae spiders (Entelegynae): interspecific karyotype diversity in Argyrodes and diploid number intraspecific variability in Nesticodes rufipes Genet. Mol. Biol.
Stavale,Leila Miguel; Schneider,Marielle Cristina; Araujo,Douglas; Brescovit,Antonio Domingos; Cella,Doralice Maria.
Theridiidae is a derived family within the Araneoidea clade. In contrast to closely related groups, the 2n(male) = 20+X1X2 with acro/telocentric chromosomes is the most widespread karyotype among the theridiid spiders. In this work, the cytogenetic analysis of Argyrodes elevatus revealed original chromosome features different from those previously registered for Theridiidae, including the presence of 2n(male) = 20+X with meta/submetacentric chromosomes. Most individuals of Nesticodes rufipes showed family conserved karyotype characteristics. However, one individual had a 2n(male) = 24 due to the presence of an extra chromosome pair, which exhibited regular behavior and reductional segregation during meiosis. After silver staining, mitotic cells exhibited...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome morphology; Cytogenetic; Meiosis; Nucleolar organizer region; Sex chromosome system.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400012
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Spermatogenesis and nucleolar activity in Triatoma klugi (triatomine, Heteroptera) Genet. Mol. Biol.
Costa,Laiana Cristina da; Azeredo-Oliveira,Maria Tercília Vilela de; Tartarotti,Ester.
Triatoma klugi is a Chagas disease vector in the Rio Grande do Sul State. Triatominae chromosomes are holocentric and sex chromosomes segregation is post-reductional. In this paper we describe the karyotype of male T. klugi and a meiotic analysis including the nucleolar behavior during spermatogenesis. Testis cells were analyzed after lacto-acetic orcein and silver nitrate staining. Two autosomes and the heterochromosomes presented nucleolar activity (Ag-NORs) during diplotene-diakinesis. The analysis of metaphase I and II revealed a karyotype with 2n = 20+XY. In metaphase I a prominent nucleolar mass was observed in the cell periphery and small silver grains were detected in metaphase II. During anaphase, the chromosomes segregated in parallel and a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triatoma klugi; Triatomine; Cytogenetics; Nucleolus; Karyotype; Meiosis.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300008
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Chromosome complement and meiosis in three species of the Neotropical bug genus Antiteuchus (Heteroptera, Pentatomidae, Discocephalinae) Genet. Mol. Biol.
Lanzone,Cecilia; Souza,Maria José de.
Orcein staining of spermatocytes was used to study the meiotic behavior of holocentric chromosomes in three member of the genus Antiteuchus (commonly known as stink bugs). We describe and illustrate the karyotype of Antiteuchus mixtus, A. sepulcralis and A. macraspis which were cytogenetically characterized as having a diploid number of 2n = 14 and an XY sex chromosome system showing pre-reductional meiosis for autosomes and post-reductional meiosis for sex chromosomes. These species were also shown to have a long diffuse stage during meiotic prophase I and aberrant harlequin-type meiocytes. The chiasma frequency was also analyzed for two of the three species studied.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Spermatogenesis; Holokinetic chromosomes; Sex chromosomes; Meiosis; Diffuse stage.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100011
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