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Prevalence of βS-globin gene haplotypes, α-thalassemia (3.7 kb deletion) and redox status in patients with sickle cell anemia in the state of Paraná, Brazil Genet. Mol. Biol.
Shimauti,Eliana LitsukoTomimatsu; Silva,Danilo Grunig Humberto; Souza,Eniuce Menezes de; Almeida,Eduardo Alves de; Leal,Francismar Prestes; Bonini-Domingos,Claudia Regina.
The aim of this study was to determine the frequency of beta S-globin gene (βS globin) haplotypes and alpha thalassemia with 3.7 kb deletion (−α3.7kb thalassemia) in the northwest region of Paraná state, and to investigate the oxidative and clinical-hematological profile of βS globin carriers in this population. Of the 77 samples analyzed, 17 were Hb SS, 30 were Hb AS and 30 were Hb AA. The βSglobin haplotypes and −α3.7kb thalassemia were identified using polymerase chain reaction.Trolox equivalent antioxidant capacity (TEAC) and lipid peroxidation (LPO) were assessed spectophotometrically. Serum melatonin levels were determined using high-performance liquid chromatography coupled to coulometric electrochemical detection. The haplotype frequencies in the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antioxidants; Hemoglobinopathies; Melatonin; Sickle cell disease; Thalassemia.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300316
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Revival of ancient marine dinoflagellates using molecular biostimulation ArchiMer
Delebecq, Gaspard; Schmidt, Sabine; Ehrhold, Axel; Latimier, Marie; Siano, Raffaele.
The biological processes involved in the preservation, viability, and revival of long‐term dormant dinoflagellate cysts buried in sediments remain unknown. Based on studies of plant seed physiology, we tested whether the revival of ancient cysts preserved in century‐old sediments from the Bay of Brest (France) could be stimulated by melatonin and gibberellic acid, two molecules commonly used in seed priming. Dinoflagellates were revived from sediments dated to approximately 150 years ago (156 ± 27, 32 cm depth), extending the known record age of cyst viability previously established as around one century. A culture suspension of sediments mixed with melatonin and gibberellic acid solutions as biostimulants exhibited germination of 11 dinoflagellate taxa...
Tipo: Text Palavras-chave: Alexandrium; Cyst; Dinoflagellate; Gibberellic acid; Melatonin; Paleoecology; Priming; Scrippsiella; Resurrection ecology.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00627/73960/73321.pdf
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Protective Role of Melatonin in Mouse Spermatogenesis Induced by Sodium Arsenite International Journal of Morphology
Bustos-Obregón,Eduardo; Poblete,Daniel; Catriao,Roberto; Fernandes,Fábio Henrique.
Arsenic is a testicular environmental toxic. Melatonin (Me), being a potent antioxidant, may reduce the damage caused by arsenic in male fertility. The effects of daily oral exposure of Sodium Arsenite (As; 7.0 mg/kg/bw); Melatonin (Me, 10.0 mg/kg/bw); Me (10.0 mg/kg/bw) plus As (7.0 mg/kg/bw), and Negative Control (NaCl 0.9%) in male CF-1 adult mice were assessed in acute (8.3 days), chronic (33.2 days) and recovery (66,4 days) of testicular damage. We evaluated changes in testicular weight and histopathological, morphometric measurements, expression of COX-2 and Androgen Receptor (AR) antigens and lipid peroxidation levels. Treatment resulted in decreased tubular diameter and AR expression, and increased: interstitial area, luminal diameter, COX-2...
Tipo: Journal article Palavras-chave: Mouse; Arsenic; COX-2; Androgen receptor; Oxidative stress; Melatonin.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300012
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Effects of Melatonin on Tibia Bone Defects in Rats International Journal of Morphology
Koparal,M; Irtegün,S; Alan,H; Deveci,E; Gülsün,B; Pektanç,G.
The aim of this study was to evaluate the effects of melatonin healing in a tibial bone defect model in rats by means of histopathological and immunohistochemistry analysis. Twenty one male Wistar albino rats were used in this study. In each animal, bone defects (6 mm length ) were created in the tibias. The animals were divided into three groups. In group 1 control group (rats which tibial defects). Group 2 melatonin (10 mg/kg) + 14 days in the tibial defect group) was administered intraperitoneally to rats. Group 3 melatonin (10 mg/kg) + 28 days in the tibial defect group) was administered intraperitoneally to rats. Histopathological analysis of samples was performed to evaluate the process of osteoblastic activity, matrix formation, trabecular bone...
Tipo: Journal article Palavras-chave: Tibial defects; Melatonin; Osteonectin; Osteopontin.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200053
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ULTRASTRUCTURAL ANALYSIS OF PINEALECTOMY AND LACK OF LIGHT INFLUENCE OVER COLLAGEN IN THE ENDOMETRIUM OF RATS International Journal of Morphology
Pinto de Medeiros,Juliana; Wanderley Teixeira,Valéria; Aguiar Coelho Teixeira,Álvaro; Baratella Evêncio,Liriane; Evêncio Neto,Joaquim.
Some studies have shown that pinealectomized female rats present a large concentration of collagen fibers in the endometrium. The lack of light results in morphologic alterations in the uterine lamina propria and in the muscular layer, reducing thus the uterine weight. Therefore, this work aimed to analyze ultra-structurally the influence of melatonin in the remodeling of the collagen in the endometrium, in the estrus phase, in pinealectomized adult rats or submitted to lack of light. The experimental groups: I- sham-pinealectomized maintained in a clear/dark cycle 12/12 hours, during three months; II- pinealectomized rats maintained in a clear/dark cycle 12/12 hours, for three months; III- normal rats maintained in clear/dark cycle 12/12 hours for three...
Tipo: Journal article Palavras-chave: Melatonin; Endometrium; Pinealectomy; Collagen.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000300008
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Protective Role of Melatonin and Taurine Against Carbamazepine-induced Toxicity in Freshly Isolated Rat Hepatocytes International Journal of Morphology
Eghbal,Mohammad Ali; Taziki,Shohreh; Sattari,Mohammad Reza.
Carbamazepine is widely used in a broad spectrum of psychiatric and neurological disorders. Idiosyncratic hepatotoxicity is a well-known adverse reaction associated with carbamazepine. Hepatotoxicity is rare, but a real concern when initiating therapy. It was found that oxidative stress is a potential mechanism for carbamazepine-induced hepatotoxicity. Present study evaluated the hepato protective role of taurine and melatonin against carbamazepine-induced hepatotoxicity. Hepatocytes were prepared by the method of collagenase enzyme perfusion via portal vein. Cells were treated with 400 µM carbamazepine, 1mM taurine, and 1mM melatonin. Cell death, reactive oxygen species formation, lipid peroxidation, and mitochondrial membrane depolarization were assessed...
Tipo: Journal article Palavras-chave: Carbamazepine; Hepatotoxicity; Oxidative stress; Taurine; Melatonin.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300049
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Effects of Ischemia/Reperfusion on ß Cells of Pancreas and Protective Effects of Melatonin Treatment International Journal of Morphology
Yildirim,Ayse  ; Tuncer,Mehmet Cudi; Pamukçu,Özlem  ; Aktas  ,Ayfer  ; Akkus,Murat  .
Oxygen free radicals are considered to be important components involved in the pathophysiological tissue alterations observed during ischemia-reperfusion (I/R). In this study, we investigated the putative protective effects of melatonin treatment on pancreatic I/R injury. Sprague Dawley male rats were subjected to 30 min of pancreatic pedicle occlusion followed by 90 min reperfusion. Melatonin (10 mg/kg. s.c) was administrated 30 min prior to ischemia or I/R application. At the end of the reperfusion periods, rats were decapitated. Pancreatic samples were taken for transmission electron microscopy. The results indicated that ischemia created b cell damage as evidenced by dilatation between the nucleus inner and outer membrane and degeneration on islets of...
Tipo: Journal article Palavras-chave: Pancreas; SS cells; Melatonin; Ischemia; Reperfusion.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000200036
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Effect of Photoperiod and Melatonin on Volume and Cellular Parameters of Testis in Pinealectomized Rats International Journal of Morphology
Akosman,Murat Sirri; Özdemir,Vural; Taskiran,Nihal; Akalan,Mehmet Aydin; Bülbül,Aziz.
Mammalian reproductive axis is regulated by the combination of three fundamental tissues of neuroendocrine system including hypothalamus, hypophysis and gonads. In recent years, pineal gland has been included in this axis. The aim of the present study was to investigate the effect of 12L (Light):12D (Dark) photoperiod and melatonin administration (0.5 mg/kg/day; subcutaneously) on testicular volume and cellular parameters of testis at the pinealectomized (PE) rats. For this aim, twelve adult rats were firstly pinealectomized and then divided into two groups as GI and GII randomly. The GI rats served as control group and received only normal saline, whereas GII rats were the melatonin administered group. It was found that the total testicular volume,...
Tipo: Journal article Palavras-chave: Cavalieri Method; Melatonin; Optical Fractionator; Pinealectomy; Stereology; Testis.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000300046
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Melatonin Protective Role in Mouse Cauda Epipidymal Spermatozoa Damage Induced by Sodium Arsenite International Journal of Morphology
Bustos-Obregón,Eduardo; Poblete,Daniel; Catriao,Roberto; del Sol,Mariano; Fernandes,Fábio Henrique.
We evaluated the sperm parameters such as cauda epididymis weight, sperm count, sperm morphology and sperm DNA stability of adult CF-1 male mice treated daily (oral exposure) with the toxic sodium arsenite (As, 7.0 mg/kg/body weight); Melatonin (Me, 10.0 mg/kg/bw), Me (10.0 mg/kg/bw) plus As (7.0 mg/kg/bw) and Negative Control (NaCl 0.9%) to assess acute (8.3 days), chronic (33.2 days) and recovery of testicular damage (66.4 days). Arsenic decreases the number of sperm from chronic treatment (33.2 days) and this effect continued until 66.4 days of treatment. The toxic effect of As also altered the morphology of spermatozoa in all treatment periods when compared to the negative control group. However, Metalonin induced protective effects in periods of 33.2...
Tipo: Journal article Palavras-chave: Mouse; Melatonin; Arsenic; Sperm; DNA stability.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400017
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Protective Effect of Melatonin on Damage in the Sperm Parameters of Mice Exposed to Diazinon International Journal of Morphology
Sarabia,L; Espinoza-Navarro,O; Maurer,I; Ponce,C; Bustos-Obregón,E.
Since normal sperm parameters can be altered by organophosphorous pesticides, this study intended to determine if melatonin is able to prevent the damage on sperm quality after an acute exposure to diazinon. Adult male mice were injected intraperitoneally with melatonin, diazinon (1/3 or 2/3 LD50) or both, and sperm parameters were evaluated on days 1 or 32 post injection. Groups treated with diazinon showed elevated lipid peroxidation levels on day 1 post treatment, while groups pretreated with melatonin before diazinon showed no difference compared to control. Sperm count showed a significant decrease in both diazinon-treated groups only on day 32 post injection; no differences were observed in groups pretreated with melatonin prior to diazinon compared...
Tipo: Journal article Palavras-chave: Melatonin; Organophosphates; Antioxidant; Mouse sperm; Sperm morphology.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000400029
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Protective Effects of Melatonin on Testicular Torsion and Detorsion Damage in Sprague-Dawley Rats International Journal of Morphology
Aktas,Ayfer; Tuncer,M. Cudi; Yildirim,Ayse; Nergiz,Yusuf; Akkus,Murat.
In this study, we evaluated the ultrastructural findings of testis with systemic administration of different doses of melatonin during ischemic period in a rat model of testicular torsion/detorsion (T/D). Testis ischemia-reperfusion (I/R) injury was induced by torsion of the left testis, with a 720 degrees twisting of the spermatic cord so as to produce a total occlusion of testis for 2.5 hours. Subsequently, the same testis was then detorsioned. According to surgical procedure in each group, unilateral orchiectomies were performed for histopathologic examination. The groups were labelled as control group, torsion group (T), torsion and detorsion group (T/D), torsion-detorsion and melatonin group (T/D+20,50 and 100 mg/kg melatonin). For the histological...
Tipo: Journal article Palavras-chave: Testis; Torsion; Detorsion; Spraque-Dawley rat; Melatonin.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000100001
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Melatonin in maturation media fails to improve oocyte maturation, embryo development rates and DNA damage of bovine embryos Scientia Agricola
Takada,Luciana; Martins Junior,Alicio; Mingoti,Gisele Zoccal; Balieiro,Julio César Carvalho; Coelho,Lia Alencar.
Melatonin (MEL) acts as a powerful scavenger of free radicals and direct gonadal responses to melatonin have been reported in the literature. Few studies, however, have evaluated the effect of MEL during in vitro maturation (IVM) on bovine embryos. This study tested the addition of MEL to maturation medium (MM) with no gonadotropins on nuclear maturation and embryo development rates and the incidence of DNA damage in resulting embryos. Cumulus-oocyte complexes were aspirated from abattoir ovaries and cultured in MM (TCM-199 medium supplemented with 10% fetal calf serum - FCS) at 39ºC and 5% CO2 in air. After 24 hours of culture in MM with 0.5 µg mL-1 FSH and 5.0 µg mL-1 LH; 10-9 M MEL) or 10-9 M MEL, 0.5 µg mL-1 FSH and 5.0 µg mL-1 LH, the oocytes were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Comet assay; Embryos; Gonadotropins; In vitro fertilization; Melatonin.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162010000400003
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Physiological effect of melatonin, IAA and their precursor on quality and quantity of chickpea plants grown under sandy soil conditions CIGR Journal
Dawood, Mona Gergis.
The physiological effect of melatonin, IAA and their precursor (tryptophan) as seed priming on quantity and quality of chickpea plants was investigated during two growing seasons of 2013/2014 and 2014/2015 under sandy soil conditions at the Research and Production Station, National Research Centre, Nubaria Province, Behaira Governorate, Egypt. Data show that melatonin treatments showed the most pronounced effect on vegetative growth parameters followed by IAA and tryptophan treatments. The highest significant increase in dry weight of shoot/plant was achieved by melatonin treatments at 0.5 and 1.0mM. Moreover, melatonin treatments at 0.25 and 0.5 mM were the most pronounced treatments caused the highest significant increase in total photosynthetic pigments...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Melatonin; IAA; Tryptophan; Chickpea; Sandy soil.
Ano: 2018 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/4377
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Comparison of sexual performance and testicular characteristics of melatonin treated Kivircik and Charollais rams during the non-breeding season Arq. Bras. Med. Vet. Zootec.
Cevik,M.; Yilmazer,C.; Kocyigit,A..
ABSTRACT This study was conducted to investigate the effects of melatonin implantation on basic testicular characteristics and reproductive performance of Kivircik and Charollais rams and ewes during the non-breeding season. In this study, 8 Kivircik and 6 Charollais rams were used. Rams were implanted with 54 mg melatonin according to the manufacturer's instructions. At melatonin implantation and at ram introduction the reproductive performance and testicular characteristics were evaluated. Throughout the experimental period, rams were permanently kept outdoors under conditions of natural day length and at ambient temperature. The effects of exogenous melatonin treatments on the reproductive performances of rams and ewes, estrus response, pregnancy rate,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ram; Melatonin; Fertility; Non-breeding season.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352017000200278
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Effects of hypoxia on epididymal sperm parameters and protective role of ibuprofen and melatonin Biol. Res.
Vargas,Álvaro; Bustos-Obregón,Eduardo; Hartley,Ricardo.
Hypobaric hypoxia is of interest due to an increase of human populations working at high altitude. Testicular damage is related to the physiological response (neoangiogenesis) to increased intrascrotal blood flow as temperature rises. Hypoxia is a stress factor with overproduction of reactive oxygen species (ROS). The effect of hypoxia in mice reproductive parameters is analyzed. Animals were exposed to simulated hypoxia of 4,200 meters above sea level (m.a.s.l.) in a chamber for 33.2 days, both to continuous (HH) or intermittent hypoxia (HI) with an intermittency period of 4 days hypoxia /4 days normoxia (500 m.a.s.l.). The anti-inflammatory drug Ibuprofen was administered to a group of mice to control vasodilation and increased blood flow. Melatonin was...
Tipo: Journal article Palavras-chave: Epididymal sperm; Mouse; Ibuprofen; Melatonin.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200008
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Melatonin Protects the Heart, Lungs and Kidneys from Oxidative Stress under Intermittent Hypobaric Hypoxia in Rats Biol. Res.
Farías,Jorge G; Zepeda,Andrea B; Calaf,Gloria M.
Melatonin (N-acetyl-5-methoxytryptamine) is the main secretory product of the pineal gland in all mammals including humans, but it is also produced in other organs. It has been previously demonstrated to be a powerful organ-protective substance under oxidative stress conditions. The aim of this study was to evaluate the protective effect of melatonin in several organs such as heart, lung, kidney, and of the reproductive system, such as testis and epididymis in animals exposed to intermittent hypobaric hypoxia and therefore exposed to oxidative stress and analyzed by lipid peroxidation. Ten-week-old male Wistar rats were divided into 6 groups for 96 hours during 32 days under: 1) Normobaric conditions, 2) plus physiologic solution, 3) plus melatonin, 4)...
Tipo: Journal article Palavras-chave: Intermittent hypobaric hypoxia; Lipid peroxidation; Melatonin; Oxidative stress.
Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100011
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The Circadian Timing System: Making Sense of day/night gene expression Biol. Res.
RICHTER,HANS G; TORRES-FARFÁN,CLAUDIA; ROJAS-GARCÍA,PEDRO P; CAMPINO,CARMEN; TORREALBA,FERNANDO; SERÓN-FERRÉ,MARÍA.
The circadian time-keeping system ensures predictive adaptation of individuals to the reproducible 24-h day/night alternations of our planet by generating the 24-h (circadian) rhythms found in hormone release and cardiovascular, biophysical and behavioral functions, and others. In mammals, the master clock resides in the suprachiasmatic nucleus (SCN) of the hypothalamus. The molecular events determining the functional oscillation of the SCN neurons with a period of 24-h involve recurrent expression of several clock proteins that interact in complex transcription/translation feedback loops. In mammals, a glutamatergic monosynaptic pathway originating from the retina regulates the clock gene expression pattern in the SCN neurons, synchronizing them to the...
Tipo: Journal article Palavras-chave: Biological rhythms; Circadian timing system; Clock genes; Melatonin.
Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100003
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Down-Regulation of NFkB, Bax,TGF-β, Smad-2mRNA expression in the Livers of Carbon Tetrachloride Treated Rats using Different Natural Antioxidants BABT
Al-Rasheed,Nouf Mohamed; Fadda,Laila Mohamed; Al-Rasheed,Nawal Mohamed; Ali,Hanaa Mahmoud; Yacoub,Hazar Ibrahim.
The objective of this study is to examine whether silymarin alone or in combination with chlorogenic acid and/ or melatonin plays a modulatory role against apoptotic damage in rats liver induced by of CCl4. The present work revealed that CCl4 induced elevation of in Bax, Smad, TGF-β and NFkBhepatic mRNA expression, administration of silymarin alone down regulates these expressions. Treatment with chlorogenic acid and/ or melatonin along with silymarin produced best results in this concern. Bcl-2 expression was down regulated by CCl4 whereas concurrent treatment of chlorogenic acid and/ or melatonin along with silymarin increased this expression. On conclusion, the use of chlorogenic acid and/ or melatonin potentiates the anti-apoptotic action of silymarin.
Tipo: Info:eu-repo/semantics/article Palavras-chave: CCl4; Silymarin; Chlorogenic acid; Melatonin; MRNA expression.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100408
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The effect of melatonin and/or complex vitamin B1,B6,B12 in modulating epinephrine-induced stress in male rats BABT
EL-Toweissy,Mona Yossef; Mohamed,Nema Abdel-Hameed; Abdel-Wahab,Wessam Mohamed.
This study was undertaken to determine the modulating effects of intramuscular administration of melatonin (MT)(1mg/ kg) and/or Tri-B (B 1 , B 6 and B 12 ) (20mg/kg) on body weight and some biochemical changes in rats induced by Epinephrine (Epi) injection. The data showed that MT and/or Tri-B treatment effectively improved the changes in malondialdehyde, lipid profile, blood sugar level and insulin. MT and/or Tri-B administration following Epi improved partially the decrease in body weight and liver glycogen levels. Tri B injection following Epi partialy improved all the tested parameters except malondialdehyde and blood sugar level that completely improved in stressed rats. It was evident that a combination of MT and vitamin B complex had protective...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Stress; Epinephrine; Melatonin; Tri-B; Rats.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000300007
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Role of Carnosine and Melatonin in Ameliorating Cardiotoxicity of Titanium Dioxide Nanoparticles in the Rats BABT
Al-Rasheed,Nouf; Faddah,Laila; Ibrahim,Hanan; Mohamed,Azza M; Al-Rasheed,Nawal; Abdelbaky,Nayira.
The aim of this work was to study the possible cardiotoxicity of two different doses of 50 nm nano titanium dioxide (n-TiO2) and the possible modulating effects of the use of two natural antioxidants carnosine and melatonin. The results showed that TiO2- NPs produced deleterious effects on rat cardiac tissue as confirmed by the increased levels of serum myoglobin, troponin-T and CK-MB. Increased levels of serum Inflammatory markers represented by the tumor necrosis factor alpha (TNF-α) and Interleukin-6 (IL-6) was also noticed. Caspase3 and IGg were elevated compared to the control group in a dose dependant manner. treatment of the rats with Carnosine or melatonin. along with TiO2- NPs administration significantly improved most of the elevated biochemical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Titanium dioxide nanoparticles; Cardiotoxicity; Carnosine; Melatonin.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000400577
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