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| Quiroz,Karla A.; Berrios,Miguel; Carrasco,Basilio; Retamales,Jorge B.; Caligari,Peter D. S.; García-Gonzáles,Rolando. |
| Abstract Background: Vegetative propagation of Fragaria sp. is traditionally carried out using stolons. This system of propagation, in addition to being slow, can spread plant diseases, particularly serious being viral. In vitro culture of meristems and the establishment of micropropagation protocols are important tools for solving these problems. In recent years, considerable effort has been made to develop in vitro propagation of the commercial strawberry in order to produce virus-free plants of high quality. These previous results can serve as the basis for developing in vitro-based propagation technologies in the less studied species Fragaria chiloensis. Results: In this context, we studied the cultivation of meristems and establishment of a... |
| Tipo: Journal article |
Palavras-chave: Fragaria chiloensis; Meristem culture; Plant growth regulators; Virus elimination; Plant morphogenesis. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602017000100404 |
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| Milosevic,Snezana; Subotic,Angelina; Bulajic,Aleksandra; Djekic,Ivana; Jevremovic,Sladjana; Vucurovic,Ana; Krstic,Branka. |
| The possibility for obtaining virus free plants from Impatiens hawkerii Bull. shoots infected with Tomato spotted wilt virus (TSWV) through meristem-tip culture was examined. TSWV presence in I. hawkerii plants was detected by DAS-ELISA and RT-PCR and identification of the virus was confirmed by sequencing one of the chosen isolate (GenBank Accesion CQ132190). Meristem-tip explants (0.3-1.5 mm) from virus-infected shoots are cultured on MS media supplemented with different concentrations of the cytokinins, CPPU or TDZ (0.01-1.0 µM), respectively. Using this system, a large number of in vitro shoots could be produced from a single explant. Also, cytokinins showed a stimulatory effect on the length, fresh and dry weights of the newly formed shoots. Plant... |
| Tipo: Journal article |
Palavras-chave: Meristem culture; New Guinea Impatiens; Tomato spotted wilt virus; Virus- free plants. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100003 |
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