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Respuesta de la simbiosis tripartita Rhizobium-Leguminosa-Micorriza arbuscular ante vanadio y níquel. Colegio de Postgraduados
Vidal Fernández, Judith Estela.
Este trabajo evaluó la respuesta de la simbiosis tripartita Rhizobium-leguminosa-micorriza arbuscular en presencia de vanadio (V) y níquel (Ni). El trabajo se dividió en dos fases experimentales; la primera fase estudió el comportamiento de la simbiosis Rhizobium tropici-Phaseolus vulgaris ante concentraciones de V o Ni (0, 25, 50, 75, 100 y 120 mg L-1) y su efecto en el contenido elemental en nódulos, en un sistema de bolsa de crecimiento bajo condiciones controladas (25°C, 70% HR y 12h de fotoperiodo) durante 30 días. En las plantas con V, la nodulación inició al cuarto día después de la inoculación y tuvo un máximo de 40 nódulos por planta en el testigo y 6 nódulos en las concentraciones de 50 y 100 mg L-1. La mayor actividad nitrogenasa (AN) se...
Palavras-chave: Nodulación; Metales; Fitorremediación; Simbiosis; Phaseolus; Rhizophagus; Nodulation; Metals; Phytorremediation; Symbiosis; Edafología; Maestría.
Ano: 2012 URL: http://hdl.handle.net/10521/753
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Dissolved and particulate metals (Fe, Zn, Cu, Cd, Pb) in two habitats from an active hydrothermal field on the EPR at 13 degrees N ArchiMer
Sarradin, Pierre-marie; Lannuzel, D; Waeles, M; Crassous, Philippe; Le Bris, Nadine; Caprais, Jean-claude; Fouquet, Yves; Fabri, Marie-claire; Riso, R.
The distribution of Fe, Cu, Zn, Pb, Cd between the dissolved (<2 mu m) and the particulate (>2 mu m) fractions was measured after in-situ filtration in two hydrothermal habitats. The total metal concentration ranges exhibit a clear enrichment compared with the seawater concentration, accounting for the hydrothermal input for all the metals considered. Iron is the predominant metal (5-50 mu M) followed by Zn and Cu. Cd and Pb are present at the nM level. At the scale studied, the behavior of temperature, pH and dissolved iron is semi-conservative whereas the other dissolved and particulate metals are characterized by non-conservative patterns. The metal enrichment of the >2 mu m fraction results from the settlement and accumulation of particulate...
Tipo: Text Palavras-chave: Hydrothermal fauna; Habitats; Particulate; Dissolved; Metals.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3830.pdf
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L'utilisation des échantillonneurs passifs. Une nouvelle méthode pour évaluer la contamination chimique des masses d'eau ArchiMer
Gourlay, Catherine; Gonzalez, Jean-louis.
The Water European Framework Directive requires the implementation of programs to monitor the chemical quality of water bodies (freshwater, transition water and seawater), based on grab measurements of concentrations of various contaminants (metals, organic compounds). The results are limited by several analytical difficulties, and the poor representativity because of the temporal variability of contamination. The passive sampling techniques, which basically consist of concentrating substances on a submerged device for a given period, and analyzing accumulated substances, should improve the monitoring, by simplifying analytical issues (lower detection limits, analysis in a simpler matrix), and allowing time integration of the contamination. Here, we...
Tipo: Text Palavras-chave: Échantillonneurs passifs; Eau; Membrane semi-perméable (SPMD); Gradient de diffusion en couche mince (DGT); Métaux; HAP; Passive samplers; Water; Semipermeable membrane device (SPMD); Diffusive gradient in thin film (DGT); Metals; PAHs.
Ano: 2010 URL: https://archimer.ifremer.fr/doc/00077/18850/16423.pdf
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Mineral and organic soluble forms of zinc, iron and copper: Distribution in the Seine Estuary. ArchiMer
Gandon, R; Guegueniat, P; Fischer, J; Wartel, M.
One characteristic of the Seine Estuary system is the process of sediment resuspension by the specific hydrodynamics of this environment. It's the main factor of trace metals transport. Autotrophic activities also have an effect on the estuary, by pH variation. The behavior of soluble and organic copper, iron, have been studied in relation to salinity in and out of the estuary. The results are compared with those of other estuaries.
Tipo: Text Palavras-chave: Hydrodynamics; Physicochemical properties; Water properties; Dissolved organic matter; Biogeochemical cycle; Resuspension; Metals; Suspended particulate matter.
Ano: 1985 URL: http://archimer.ifremer.fr/doc/1985/acte-1300.pdf
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Relationship between the occurrence of filamentous bacteria on Bathymodiolus azoricus shell and the physiological and toxicological status of the vent mussel ArchiMer
Martins, I.; Colaco, A.; Santos, Raphael; Lesongeur, Francoise; Godfroy, Anne; Sarradin, Pierre-marie; Cosson, R. P..
The edifice walls of the Eiffel Tower hydrothermal vent site (Mid-Atlantic Ridge, Lucky Strike vent field) are populated with dense communities of dual symbioses harboring vent mussel Bathymodiolus azoricus, some of which are covered by white filamentous mats belonging to sulfur-oxidizing bacteria. Mussels were collected in both the presence and absence of the filamentous bacteria. A sample of the filamentous bacteria was collected and water measurements of temperature, CH4 and H2S were recorded at the collection area. The whole soft tissues were analyzed for total lipid, carbohydrate and total protein. Metallothioneins and metals (Cu, Fe and Zn) levels were determined in the major organs. The results showed no significant physiological and toxicological...
Tipo: Text Palavras-chave: Vent mussel; Metals; Metallothioneins; Filamentous bacteria; Eiffel Tower; Biomarkers.
Ano: 2009 URL: http://archimer.ifremer.fr/doc/2009/publication-6821.pdf
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Evaluation des effets délétères des métaux et des pesticides sur les gamètes et les premiers stades de développement de l’huître creuse, Crassostrea gigas : application à la problématique de la pollution du Bassin d’Arcachon ArchiMer
Mai, Huong.
The coastal areas are subject to multiple anthropogenic pressures including chemical pollution that can pose a real risk to the sustainability of aquatic species. The Arcachon Bay, macrotidal lagoon located on the French Atlantic coast, is the important ecosystem for oyster farming. But for several years, the oyster farms face lower recruitment and high mortality of oyster spat. Chemical contamination of the environment as a factor that may contribute to the observed effects on oysters has so far not been investigated.The present thesis aimed at evaluating through different approaches, of the potential toxicity of heavy metals and pesticides representative of the Arcachon Bay contamination on the early life stages of the Pacific oyster, Crassostrea gigas....
Tipo: Text Palavras-chave: Métaux; Huitre creuse; Test embryo-larvaire; Génotoxicité; Test de fertilité; Expression de gènes; Comet assay; Gene expression; Fertilization assay; Pesticides; Metals; Pacific oyster; Embryo-larvae toxicity assay.
Ano: 2013 URL: https://archimer.ifremer.fr/doc/00590/70183/68199.pdf
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Spatial variation of metal bioaccumulation in the hydrothermal vent mussel Bathymodiolus azoricus ArchiMer
Cosson, R; Thiebaut, E; Company, R; Castrec Rouelle, M; Colaco, A; Martins, I; Sarradin, Pierre-marie; Bebianno, M.
The variability of the bioaccumulation of metals (Ag, Cd, Cu, Fe, Mn and Zn) was extensively studied in the mussel Bathymodiolus azoricus from five hydrothermal vent sites inside three main vent fields of increasing depth along the Mid-Atlantic Ridge: Menez Gwen, Lucky Strike and Rainbow. Metal bioaccumulation varied greatly between vent fields and even between sites inside a vent field with B. azoricus showing a great capacity to accumulate metals. The bioaccumulation of these metals also varied significantly among tissues. The main target was the gills where metals were mainly associated with soluble compounds whereas in the digestive gland they were mainly associated with insoluble compounds. Storage of metals under insoluble forms in B. azoricus seems...
Tipo: Text Palavras-chave: Bathymodiolus; Mytilids; Hydrothermal vents; Azores; Metals; Bioaccumulation.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-3954.pdf
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TOPHYPAC. Tolérance des communautés phytoplanctoniques aux phytosanitaires dans le panache de la Charente. Programme Évaluation et réduction des risques liés à l’utilisation des Pesticides ArchiMer
Stachowski-haberkorn, Sabine; Guesdon, Stephane; Bechemin, Christian; Chiffoleau, Jean-francois; Brach-papa, Christophe; Soudant, Philippe; Beker, Beatriz; Jadas-hécart, Alain.
TOPHYPAC is a field study that was conducted in the Charente river estuary (Marennes-Oléron bay). The main goal was to assess the possible impact of chemical contamination with copper and pesticides on phytoplankton communities. Physical, chemical (nutrients, metals and pesticides) and biological (phytoplankton community) parameters were monitored in the field during three years, from 2011 to 2014. In 2012 and 2013, field sampling was coupled with experimental exposure of phytoplankton communities to copper and to an herbicide cocktail, after transportation to the lab: the effects were assessed using photosynthesis endpoint and cellular densities. TOPHYPAC allowed to set the framework of Charente estuary contamination with pesticides: herbicides glyphosate...
Tipo: Text Palavras-chave: Continuum eau douce/eau côtière; Pesticides; Métaux; Communautés phytoplanctoniques; Efficacité de photosynthèse; PICT; Tolérance; Cytométrie en flux; Toxicité chronique; Freshwater/coastal water continuum; Pesticides; Metals; Phytoplankton communities; Photosynthesis efficiency; PICT; Tolerance; Flow cytometry; Chronic toxicity.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00300/41115/40289.pdf
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Utilisation des métallothionéines comme biomarqueur de la contamination métallique : variabilité entre sites et organes chez l'huı̂tre Crassostrea gigas ArchiMer
Geret, F; Cosson, Rp.
Heavy metals (Cd, Cu, Hg and Zn) and metallothionein levels were determined in oysters Crassostrea gigas from two sites: the Gironde Estuary (contaminated site) and Bourgneuf Bay (uncontaminated site). Metallothionein levels led us to suspect the presence of cadmium in the environment when quantified in the gills. Soluble cadmium and metallothioneins were positively correlated in the digestive gland of oysters from Gironde (contaminated site), but metallothionein levels in this organ were not significantly different from levels observed in the digestive gland of oysters from Bourgneuf Bay (uncontaminated site). Conversely, no correlation was noticed between metals and metallothioneins in the gills of oysters from Gironde despite metallothionein levels...
Tipo: Text Palavras-chave: Métallothionéines; Métaux; Crassostrea gigas; Biomarqueur; Metallothioneins; Metals; Crassostrea gigas; Biomarker.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00324/43561/44058.pdf
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Cellular and biochemical responses of the oyster Crassostrea gigas to controlled exposures to metals and Alexandrium minutum ArchiMer
Haberkorn, Hansy; Lambert, Christophe; Le Goic, Nelly; Quere, Claudie; Bruneau, A; Riso, Ricardo; Auffret, Michel; Soudant, Philippe.
Effects of simultaneous exposure of Pacific oyster, Crassostrea gigas, to both a harmful dinoflagellate that produces Paralytic Shellfish Toxins (PST), Alexandrium minutum, and cadmium (Cd) and copper (Cu), were assessed. Oysters were exposed to a mix of Cd-Cu with two different diets (i.e. A. minutum or Tisochrysis lutea) and compared to control oysters fed A. minutum or T. lutea, respectively, without metal addition. Metals and PST accumulations, digestive gland lipid composition, and cellular and biochemical hemolymph variables were measured after 4 days of exposure. Oysters exposed to Cd-Cu accumulated about thirty-six times less PSTs than oysters exposed to A. minutum alone. Exposure to Cd-Cu induced significant changes in neutral lipids (increase in...
Tipo: Text Palavras-chave: Harmful algae; Metals; Oysters; Toxin accumulation; Physiological effects.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00169/28071/26286.pdf
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Relationship between metal levels in the vent mussel Bathymodiolus azoricus and local microhabitat chemical characteristics of Eiffel Tower (Lucky Strike) ArchiMer
Martins, Ines; Cosson, Richard P.; Riou, Virginie; Sarradin, Pierre-marie; Sarrazin, Jozee; Santos, Ricardo S.; Colaco, Ana.
The turbulent mixing of hydrothermal hot fluid with cold seawater creates large chemical gradients at a small spatial scale that may induce variable physiological and biochemical adaptations within the vent fauna. The adaptation to such a variable environment by the vent mussel Bathymodiolus azoricus relies on a dual symbiosis hosted in the gills, and digestion of particulate organic matter. The surrounding environment not only provides the necessary energy sources and suspended organic particles for the vent mussel nutrition, but also potentially toxic compounds such as metals. Our main goal was to see if there is a relation between metal accumulation in mussel organs and the chemical characteristics of their close environment. Mussels were collected at...
Tipo: Text Palavras-chave: Metals; Metallothioneins; Bathymodiolus azoricus; Hydrothermal vent; Environmental conditions; Physiological condition; Spatial distribution.
Ano: 2011 URL: http://archimer.ifremer.fr/doc/00030/14097/11327.pdf
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Traitement par lagunage a haut rendement algal (LHRA) des effluents piscicoles marins recycles: évaluation chimique et écotoxicologique ArchiMer
Deviller, Genevieve.
World aquaculture is a fast growing activity and the control of its waste becomes a key issue. Compared to traditional systems, recirculating systems allow to reduce replacement water and waste volumes. Nevertheless their effluents are more concentrated in dissolved nutrients (nitrate, phosphate). The high rate algae pond (HRAP) is one of the possible treatment technique to remove those nutrients. During this work, three rearing systems: one recycled, one recycled with HRAP and one open as a standard, were operated for one year to assess the impact of: (1) the reuse of treated effluent on survival, growth and nutrition of sea bass (2) the rearing system on fish health using multibiomarkers and trace metals accumulation responses and (3) best conditions for...
Tipo: Text Palavras-chave: Aquaculture; Fish; Growth; Metals; Biomarker; Water treatment; Recirculating effluent; High rate algal pond; Aquaculture; Poisson; Croissance; Métaux; Biomarqueur; Epuration; Effluent recyclé; Lagunage à haut rendement algal.
Ano: 2003 URL: http://archimer.ifremer.fr/doc/2004/these-163.pdf
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Evolution de la complexation des metaux (Ca, Mg, Cd, Pb et Cu) dans l''estuaire de la Seine ArchiMer
Fischer, J; Wartel, M.
Interaction between metallic ions and dissolved organic matter can take major part in the biogeochemical cycle of metals in the aquatic environment. The authors point out copper behavior by the study of complexing capacity of surface and bottom waters. A systematic analysis of major elements in natural waters has allowed for a proposal of geographical distribution of metals.
Tipo: Text Palavras-chave: River plumes; Dissolved organic matter; Chemical reactions; Salinity; Biogeochemical cycle; Metals; Ion association; Water properties.
Ano: 1985 URL: http://archimer.ifremer.fr/doc/1985/acte-1299.pdf
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Distribution and behavior of dissolved hydrogen sulfide in hydrothermal plumes ArchiMer
Radford Knoery, Joel; German, C; Charlou, Jean-luc; Donval, Jean-pierre; Fouquet, Yves.
Through the deep ocean, hydrothermal plumes disperse high concentrations of key chemical tracers including He-3, CH4, Mn, Fe, H2S, etc. This paper focuses on the distribution and behavior of total dissolved sulfide (sulfide hereafter) in hydrothermal plumes to show that its plume concentration decreases to subnanomolar a few kilometers from the vents. We also report on sulfide removal rates determined at in situ conditions; we observe that they are two orders of magnitude greater than for open ocean seawater, consistent with sulfide being detected only in the vicinity of hydrothermal vents. From our observations, we infer that the sole presence of sulfide in hydrothermal plumes locates active venting at the kilometer scale.
Tipo: Text Palavras-chave: Chemical oceanography; Mid ocean ridges; Metals; Helium isotopes; Methane; Tracers; Hydrogen sulphide; Plumes; Hydrothermal activity.
Ano: 2001 URL: https://archimer.ifremer.fr/doc/2001/publication-688.pdf
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Influence hydrothermale dans les sédiments de fosses de la mer Rouge par l'étude de quelques éléments traces ArchiMer
Pierret, Marie Claire; Clauer, Norbert; Blanc, Gerard.
The Cu, Ni, Fe, Cr and Zr concentrations were evaluated to study the influence of hydrothermal activity on the sediments of six Red-Sea deeps. Comparison between the Ni/Fe and Cu/Fe ratios supports distinction between hydrothermal and non-hydrothermal sediments, whereas anti-correlation between Zr and Cr concentrations results from hydrothermal influence. The results allow us to envisage that the sediments of the Suakin Deep did not undergo a hydrothermal activity and that this hydrothermal influence was weak in the Valdivia Deep. The sediments of Port-Soudan Deep recorded a hydrothermal activity, whereas this activity was the strongest in the Thetis and Atlantis II deeps.
Tipo: Text Palavras-chave: Sédiment; Mer Rouge; Fosses; Hydrothermalisme; Métaux; Sediment; Red sea; Deeps; Hydrothermalism; Metals.
Ano: 2000 URL: http://archimer.ifremer.fr/doc/00323/43375/42836.pdf
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L'arsenic en milieu marin: Synthese bibliographique ArchiMer
Michel, Pierre.
Arsenic is an important element in marine environment due to its level 1,000 times higher that of mercury and its behavior close to that of phosphorus. This overview recalls few chemical and biochemical basis and presents the current state of knowledge about its sources, concentration levels in estuarine and oceanic waters, sediments and biota. Toxicity for species, ecosystems and consumers, and analytical methods for its control are approached.
Tipo: Text Palavras-chave: Environmental impact; Literature reviews; Toxicity; Arsenic; Metals.
Ano: 1985 URL: http://archimer.ifremer.fr/doc/1985/publication-1779.pdf
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METROC : évaluation des apports de contaminants chimiques de la métropole marseillaise au milieu marin ArchiMer
Jany, Cassandre; Zebracki, Mathilde.
METROC program (part of the MEDICIS program about study and fate of the chemical contamination in Western Mediterranean Sea) focuses on sources and wastes issued by large metropolis, with the agglomeration of Marseille as a pilot site. This report presents the results of a sampling campaign in rivers and urban discharges in Marseille and on different types of contaminants (metals, PAH, PCB and PBDE) in dissolved and particulate phases, in dry and rain periods. Results show that concentrations of pollutants are greater in Aygalades river (metals and organic contaminants) and in Emissaire 2, sewer overflows and torrents. From the results of the analysis and discharge given by the DEA, we estimated partially the flows of pollutants which show that the first...
Tipo: Text Palavras-chave: Mer Méditerranée; Littoral; Apports urbains; Marseille; Contamination chimique; Métaux; HAP; PCB; PBDE; Concentrations; Mediterranean Sea; Coast; Urban waste; Marseilles; Chemical contamination; Metals; PAH; PCB; PBDE; Concentration.
Ano: 2012 URL: http://archimer.ifremer.fr/doc/00116/22692/20431.pdf
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Pouvoir complexant des eaux de l'estuaire de la Seine ArchiMer
Fischer, J; Wartel, M; Guegueniat, P; Gandon, R.
Copper has been studied, by the authors, owing to its ability to complex with various organic ligands of natural waters. Metals extraction and liberation can be explained, in part, by this complexing capacity.
Tipo: Text Palavras-chave: Estuaries; Suspended particulate matter; Dissolved organic matter; Copper; River discharge; Ion association; Metals; Biogeochemical cycle; Water properties.
Ano: 1985 URL: http://archimer.ifremer.fr/doc/1985/acte-1298.pdf
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Metal bioaccumulation and storage forms in the shrimp, Rimicaris exoculata, from the Rainbow hydrothermal field (Mid-Atlantic Ridge); preliminary approach to the fluid-organism relationship ArchiMer
Geret, Florence; Riso, Ricardo; Sarradin, Pierre-marie; Caprais, Jean-claude; Cosson, Richard P.
At the Rainbow hydrothermal site the concentrations of metals in the fluid are the highest observed in the Mid-Atlantic Ridge hydrothermal areas, and the shrimps Rimicaris exoculata were observed forming dense swarms in small depressions on the chimney walls. In order to understand the fluid-organism relationship, we investigated the relative abundance of metals in the water surrounding R. exoculata and their bioaccumulation by the shrimps. This first approach stresses a net metal enrichment around R. exoculata. Precipitation, scavenging and oxidative redissolution processes in the mixing zone are controlling the concentrations of metals. Their bioaccumulation varies according to their bioavailability in the surrounding water. Metals are abundant as...
Tipo: Text Palavras-chave: Hydrothermal fluid; Rimicaris exoculata; Metals; Bioaccumulation; Rainbow.
Ano: 2002 URL: http://archimer.ifremer.fr/doc/00186/29762/28204.pdf
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Metal concentrations in sediment and Nereis diversicolor in two Moroccan lagoons: Khnifiss and Oualidia ArchiMer
Idardare, Zaina; Chiffoleau, Jean-francois; Moukrim, A; Alla, A; Auger, Dominique; Lefrere, L; Rozuel, Emmanuelle.
Metal concentrations were measured monthly during an annual cycle (December 2004 to January 2006) in the tissues of Nereis diversicolor ragworms and sediment from two Moroccan Atlantic coastal lagoons: Oualidia in the north, situated in a highly industrialised urban area and Khnifiss in the south, far from anthropogenic influences. Relatively high levels of Zn, Ag and Cd were found in the sediment of Oualidia lagoon, while Ni concentrations were shown to be high at Khnifiss. The significant metal levels at Oualidia are a result of industrial and urban discharges, coupled with discharges from adjacent phosphate ore processing plants. Trace metal concentrations in worms vary according to season; they are usually high in winter and spring, in relation to the...
Tipo: Text Palavras-chave: Morocco; Coastal lagoons; Sediments; Nereis diversicolor; Metals.
Ano: 2008 URL: http://archimer.ifremer.fr/doc/2008/publication-6127.pdf
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