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| Chevallier, Frédérik. |
| Cryopreservation tests were carried out for Platymonas "Batan" sp., P. suesica, Isochrysis aff galbana, Chlorella minutissima, Nannochloris occulata, Chaetoceros gracilis and C. "BA" sp. The focus of the protocol was based on the duration of incorporation and cryoprotector concentration (DMSO). Two methods were used to compare the effects of each freezing protocol for algae survival. The first method allows evaluating survival rate through orange acridine coloration and epifluorescence microscope readings. The second one requires culturing each sample and growth curbs are established through daily readings of algal solutions optical density. Freezing the samples was either done directly through liquid nitrogen freezing (-196°C) or through slow freezing,... |
| Tipo: Text |
Palavras-chave: Freezing; DMSO; Liquid nitrogen; Micro algae; Congélation; DMSO; Azote liquide; Micro algues. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/rapport-1747.pdf |
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| Konoplya,Bárbara Idino; Soares,Francisco Striquer. |
| Biovolume calculation of micro algae is commonly used for the assessment in relative abundance (as biomass or carbon) of coexistent algae that vary in shape and/or size. Periphyton samples were obtained within a more general program, in fishery tanks and streams situated in the upper basin region of Tibagi River, nearby Londrina, Paraná state, south of Brazil. The present work proposes new algorithms for the determination of algal species volume. Results showed that Gyrosigma scalproides (Rabenhorst) Cleve 1894 and Tetraedron gracile (Reinsch) Hansgirg 1889 were exceptions inside their genus and the present new formula for biovolume and area gives more adequate results. In addition, new formulas applied for Phacus longicauda (Ehrenberg) Dujardin 1841 and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biovolume; Geometric models; Biomass; Micro algae. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300014 |
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