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| CORDOVA,CAIO M.M.; HOELTGEBAUM,DANIELA L.; MACHADO,LAÍS D.P.N.; SANTOS,LARISSA DOS. |
| ABSTRACT Mycoplasmas are a large group of bacteria, sorted into different genera in the Mollicutes class, whose main characteristic in common, besides the small genome, is the absence of cell wall. They are considered cellular and molecular biology study models. We present an updated review of the molecular biology of these model microorganisms and the development of replicative vectors for the transformation of mycoplasmas. Synthetic biology studies inspired by these pioneering works became possible and won the attention of the mainstream media. For the first time, an artificial genome was synthesized (a minimal genome produced from consensus sequences obtained from mycoplasmas). For the first time, a functional artificial cell has been constructed by... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mycoplasma; Molecular biology; Microbial genetics; Synthetic biology; Gene transfer techniques. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000200599 |
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| Kremer,Frederico Schmitt; McBride,Alan John Alexander; Pinto,Luciano da Silva. |
| Abstract The introduction of next-generation sequencing (NGS) had a significant effect on the availability of genomic information, leading to an increase in the number of sequenced genomes from a large spectrum of organisms. Unfortunately, due to the limitations implied by the short-read sequencing platforms, most of these newly sequenced genomes remained as “drafts”, incomplete representations of the whole genetic content. The previous genome sequencing studies indicated that finishing a genome sequenced by NGS, even bacteria, may require additional sequencing to fill the gaps, making the entire process very expensive. As such, several in silico approaches have been developed to optimize the genome assemblies and facilitate the finishing process. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microbial genetics; Molecular microbiology; Genomics; Microbiology; Draft genomes. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000400553 |
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