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Comparison of two lipid extraction methods produced by yeast in cheese whey BABT
Castanha,Rodrigo Fernandes; Morais,Lilia Aparecida Salgado de; Mariano,Adriano Pinto; Monteiro,Regina Teresa Rosim.
This work aimed to evaluate nine strains of yeast, previously identified as good producers of lipids in honey medium, for selecting the most suitable strain for the production of lipids in cheese whey medium and compared two well known extraction methods of lipids from the culture medium. The highest yield of total lipids was 1.27 g.L-1 produced by Cryptococcus laurentii 11. A comparison was made between the two culture media: cheese whey and liquid YEPG, and two lipid extraction methods: Bligh and Dyer and Folch et al. for C. laurentii. The experiments were performed with 2² full factorial design using two factors and two levels. Lipid content was higher in cheese whey and there was no difference in the extraction methods statistically. The method of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cheese whey; Yeast; Lipid extraction; Microbial oil.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400013
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Microscale direct transesterification of microbial biomass with ethanol for screening of microorganisms by its fatty acid content BABT
Sorgatto,Vanessa Ghiggi; Carvalho,Julio Cesar de; Sydney,Eduardo Bittencourt; Medeiros,Adriane Bianchi Pedroni; Vandenberghe,Luciana Porto de Souza; Soccol,Carlos Ricardo.
Abstract We present an improved method of direct transesterification suitable for the quantitative analysis of multiple dry samples for its fatty acid content, using a minimal amount of biomass and reactants. The method features an acid-catalyzed direct alcoholysis of microgram samples of dry biomass; the rationale behind the solvent and reagent proportions chosen is discussed. The method was validated using seven microbial strains with diverse lipid content (Saccharomyces cerevisiae, Saccharomyces boulardii, Candida tropicalis, Haematococcus pluvialis, Chlorella vulgaris, Spirulina platensis and Schizochytrium limacinum), and compared with a macroscale direct transesterification method, and with gravimetric analysis of lipids extracted with solvents. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbial oil; Ethanolysis; FAEE; Microscale; Screening.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100401
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