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| Demant, Carlos Alberto Rauer; Universidade Estadual Paulista “Julio de Mesquita Filho”; Auld, Dick; Texas Tech University, Lubbock, Texas, United States; Demant, Aline Rodrigues de Mello; Texas Tech University, Lubbock, Texas, United States. |
| In this study, we developed a bioassay to quantify the ricin toxin content of castor bean seeds. Existing quantification methods do not always reflect actual toxicity of the seeds analyzed, which may present lower ricin content even though they are more toxic than seeds presenting a higher content of ricin. This is because these methods actually measure the addition of ricin RCA, which is a compound less toxic than pure ricin. We decided to use in this study, the nematode Caenorhabditis elegans, which has been widely used by the pharmaceutical industry. We tested two strains of C. elegans using different methods in 8 experiments. We examined 4 methods of extracting the ricin complex and 3 methods of exposing the nematodes. Among the nematode strains and... |
| Tipo: Laboratory analysis |
Palavras-chave: Microbiologia e melhoramento genético de plantas 5.01.02.03-6 toxin; C. elegans; Selection; Ricinus microbiologia e melhoramento genetico de plantas. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/11284 |
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