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Validated microbiological and HPLC methods for the determination of moxifloxacin in pharmaceutical preparations and human plasma BJM
Abdelaziz,Ahmed A.; Elbanna,Tarek E.; Gamaleldeen,Noha M..
The article presents a comparison between microbiological and high performance liquid chromatographic (HPLC) assays for quantification of moxifloxacin in tablets, ophthalmic solutions and human plasma. The microbiological method employed a cylinder-plate agar diffusion assay using a strain of Esherichia coli ATCC 25922 as the test organism and phosphate buffer (pH8) as the diluent. The calibration curves were linear (R²&gt; 0.98) over a concentration range of 0.125 to 16 µgml-1. The within day and between days precisions were < 4.47% and < 6.39% respectively. Recovery values were between 89.4 and 110.2%. The HPLC assay used Hypersil® BDS C18 reversed phase column (250×4.6 mm, 5µm) with a mobile phase comprising 20 mM ammonium dihydrogen...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Moxifloxacin; Microbiological assay; HPLC assay; Pharmaceutical analysis; Pharmacokinetics.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400008
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Validation of an analytical method by high-performance liquid chromatography and microbiological assay, biological safety and in silico toxicity for danofloxacin BJPS
Cordeiro,Everson Willian Fialho; Hilgert,Renata Medeiros; Batista,Luiz Alcides das Chagas; Souza,Raul Oliveira; Oliveira,Luís Flávio Souza de; Machado,Michel Mansur; Malesuik,Marcelo Donadel; Paula,Fávero Reisdorfer; Stopiglia,Cheila Denise Ottonelli; Paim,Clésio Soldateli.
Danofloxacin is a veterinary fluoroquinolone used to treat respiratory and gastrointestinal diseases of birds, pigs and cattle. The literature reviewed shows some analytical methods to quantify this fluoroquinolone, but microbiological and biological safety studies are limited. The analytical methods were validated by the Official Codes. The LC-DAD method was developed and validated using an RP-18 column, mobile phase containing a mixture of 0.3% triethylamine (pH 3.0) and acetonitrile (85:15, v/v). The microbiological assay was performed by agar diffusion method (3 x 3) and Staphylococcus epidermidis as a microorganism test. Forced degradation studies were performed in both methods. The minimum inhibitory concentration (MIC) was performed by test...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Danofloxacin; Microbiological assay; Biological safety; In silico toxicology; Fluoroquinolones photodegradation.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100555
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Development and validation of a microbiological assay for determination of chlorhexidine digluconate in aqueous solution BJPS
Fiorentino,Flávia Angélica Másquio; Corrêa,Marcos Antonio; Salgado,Hérida Regina Nunes.
Chlorhexidine (CHX) is a broad-spectrum antiseptic that is used in many topical pharmaceutical formulations. Because there is no official microbiological assay reported in the literature that is used to quantify CHX, this paper reports the development and validation of a simple, sensitive, accurate and reproducible agar diffusion method for the dosage of chlorhexidine digluconate (CHX-D) in an aqueous solution. The assay is based on the inhibitory effect of CHX-D upon the strain of Staphylococcus aureus ATCC 25923, which is used as the test microorganism. The design 3x3 parallel-line model was used. The results were treated statistically by analysis of variance (ANOVA), and they were excellent in terms of linearity (r = 0.9999), presenting a significant...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agar diffusion; Antiseptic; Chlorhexidine; Microbiological assay; Quality control; Validation.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502013000200017
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Development and validation of a rapid turbidimetric assay to determine the potency of norfloxacin in tablets BJPS
Chierentin,Lucas; Salgado,Hérida Regina Nunes.
Norfloxacin is one of the first commercially available (and most widely used) fluoroquinolone antibiotics. This paper reports the development and validation of a simple, sensitive, accurate and reproducible turbidimetric assay method to quantify norfloxacin in tablets formulations in only 4 hours. The bioassay is based on the inhibitory effect of norfloxacin upon the strain ofStaphylococcus epidermidis ATCC 12228 IAL 2150 used as test microorganism. The assay was performed 3x3 parallel lines like, three tubes for each concentration of reference substance and three tubes for each sample concentration. The results were treated statistically by analysis of variance and were found to be linear (r2 = 0.9999) in the selected range of 25-100 μg mL-1; precise...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microbiological assay; Quality control; Norfloxacin/quantification in tablets; Turbidimetric method/quality control/validation; Antimicrobials/quality control.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000300629
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