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	Provedor de dados Biocell (1) BJMBR (1) PAB (1) Autor Amaral,L.M. (1) Bevacqua,Romina J. (1) Chun-Xiao,Meng (1) Costa,M.L. (1) Fernandez-Martin,Rafael (1) Mermelstein,C.S. (1) Nai-Hao,Ye (1) Rebello,M.I.L. (1) Salamone,Daniel F. (1) Zheng-Quan,Gao (1) Mais... Palavra-chave Microfilaments (3) Cell shape (1) Cytochalasin B (1) Cytoskeleton (1) Ethylene (1) Extracellular Ca2+ (1) Integrin (1) Latex yields (1) Macrolide (1) Meiotic maturation (1) Microtubules (1) Parthenogenesis (1) Physiological parameters (1) Potassium iodide (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2011 (1) 2008 (1) 2003 (1) País Brazil (2) Argentina (1) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Latex production with depolymerizing compounds of actin cytoskeleton in rubber trees PAB Zheng-Quan,Gao; Chun-Xiao,Meng; Nai-Hao,Ye. The objective of this work was to assess stimulated latex flow from rubber trees (Hevea brasiliensis) with saturated macrolide (latrunculin A), 1, 5, and 10% potassium iodide in 2% methylcellulose compared with 0.3% ethylene in 2% methylcellulose (check) and 2% methylcellulose (blank). Latex output and contents of pure rubber, total solid, sucrose, inorganic phosphorus, thiol, and Mg2+ were measured. The treatments containing 1% KI or saturated macrolide increased latex yields compared to the blank with 2% methylcellulose alone. The 1% KI or saturated macrolide treatments were equal to that of 0.3% ethylene check treatment. However, 5 and 10% KI were harmful to bark of rubber trees, even caused prolonged tapping panel dryness. Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethylene; Latex yields; Macrolide; Microfilaments; Physiological parameters; Potassium iodide. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000200018 Bovine parthenogenotes produced by inhibition of first or second polar bodies emission Biocell Bevacqua,Romina J.; Fernandez-Martin,Rafael; Salamone,Daniel F.. Parthenogenetic embryos are an ethically acceptable alternative for the derivation of human embryonic stem cells. In this work, we propose a new strategy to produce bovine parthenogenetic embryos inhibiting the emission of the first polar body during in vitro maturation, and allowing the extrusion of the second polar body during oocyte activation. Cytochalasin B, an inhibitor of actin microfilaments, was employed during in vitro maturation to inhibit first polar body emission or during parthenogenetic activation to block second polar body emission. Only one polar body was inhibited in each strategy in order to keep the diploid chromosome set. In experiment 1, the effect of cytochalasin B on in vitro maturation of bovine oocytes was evaluated. Most oocytes... Tipo: Info:eu-repo/semantics/article Palavras-chave: Meiotic maturation; Microfilaments; Cytochalasin B; Parthenogenesis. Ano: 2011 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452011000100001 Changes in cell shape, cytoskeletal proteins and adhesion sites of cultured cells after extracellular Ca2+ chelation BJMBR Mermelstein,C.S.; Rebello,M.I.L.; Amaral,L.M.; Costa,M.L.. Although much is known about the molecules involved in extracellular Ca2+ regulation, the relationship of the ion with overall cell morphology is not understood. The objective of the present study was to determine the effect of the Ca2+ chelator EGTA on the major cytoskeleton components, at integrin-containing adhesion sites, and their consequences on cell shape. Control mouse cell line C2C12 has a well-spread morphology with long stress fibers running in many different directions, as detected by fluorescence microscopy using rhodamine-phalloidin. In contrast, cells treated with EGTA (1.75 mM in culture medium) for 24 h became bipolar and showed less stress fibers running in one major direction. The adhesion plaque protein alpha5-integrin was detected by... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytoskeleton; Extracellular Ca2+; Cell shape; Microfilaments; Microtubules; Integrin. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000800018 Registros recuperados: 3 Primeira ... 1 ... Última

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