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GENETIC VARIABILITY OF THE ZEBU CATTLE BREED (Bos indicus) IN THE DEPARTAMENT OF HUILA, COLOMBIA USING MICROSATELLITE MOLECULAR MARKERS Acta biol.Colomb.
HERNÁNDEZ ESCOBAR,CARLOS; OLIVERA ÁNGEL,MARTHA; OSTOS ALFONSO,HENRY; GUERRA,MARIA TERESA.
The polymorphism of 11 microsatellites from zebu cattle (Bos indicus) was studied using a commercial multiplex system to estimate genetic variability. Allele frequencies polymorphism information content and heterozygosis were calculated. Allele frequencies revealed that in the analyzed sample the markers were not equally polymorphic. The average allele was 14.2 with the highest values for the TGLA122 microsatellites. The mean heterozygocity was 0.7056 and the polymorphism information content was 0.668. This multiplex analysis could be used for pedigree information and for adequate genetic improvements in breeding programs and paternity test.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Alleles; Microsatellite; Polymorphism; Zebu.
Ano: 2009 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2009000300013
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Species boundary and extensive hybridization and introgression in Petunia Acta Botanica
Turchetto,Caroline; Schnitzler,Carolina K.; Freitas,Loreta B..
ABSTRACT Studying the role of hybridization in the speciation of plants is one of the most thrilling areas of evolutionary biology. Hybridization in natural populations can act in opposition to divergence, contribute to adaptation through introgression or foster the emergence of new lineages via hybrid speciation. Species of the plant genus Petunia grow in open areas in southern South America. Some natural interspecific hybrid events have been described for the genus, such as between the endemic P. exserta and the widespread P. axillaris. Both species occur in sympatry in Serra do Sudeste (Brazil), where they occur in diverse habitats and exhibit floral divergence, which has been related to the attraction of different primary pollinators. The present study...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gene exchange; Hybridization; Inbreeding; Introgression; Microsatellite; Petunia; Species limits.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000400724
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Cytogenetic and molecular characterization of Speothos venaticus specimens - doi: 10.4025/actascibiolsci.v32i4.6775 Biological Sciences
Carvalho, Thaís Lima; UNESP - IBB; Curi, Rogério Abdallah; UNESP - FMVZ; Santiloni, Valquíria; UNESP - IBB; Chieregatto, Cleyde Angélica Ferreira da Silva; Sociedade dos Zoológicos do Brasil; Rocha, Guaracy Tadeu; UNESP - IBB; Mota, Lígia Souza Lima Silveira da; UNESP - IBB.
The bush dog (Speothos venaticus) is a South American canid, included in the IBAMA (Brazilian Institute of Environment and Renewable Natural Resources) official list of animals threatened with extinction, in the vulnerable category. As a preservation and conservation strategy, specimens kept in captivity by Brazilian Institutions are monitored by a management plan. In order to characterize and analyze the genetic variability of bush dog specimens, a cytogenetic analysis was carried out, and microsatellite data were also obtained through the use of 15 primers, originally developed for the domestic dog (Canis familiaris). All tested primers showed transferability and amplified fragment sizes similar to those described for the canine genome. From the total...
Palavras-chave: 2.02.04.00-0 bush dog; Canids; Chromosome; Microsatellite; Mosaicism Genética Animal bush dog; Canids; Chromosome; Microsatellite; Mosaicism.
Ano: 2010 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/6775
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Migratory connections among breeding grounds off the Eastern Pacific and feeding areas in the Antarctic Peninsula based on genotype matching Boletín de Investigaciones
Caballero,Susana; Steel,Debbie; Pallin,Logan; Botero-Acosta,Natalia; Felix,Fernando; Olavarría,Carlos; Diaz granados,Maria Claudia; Bessudo,Sandra; Friedlander,Ari; Scott Baker,C..
ABSTRACT We analysed 114 DNA samples collected in different locations within the stock G breeding grounds, including Ecuador and the northern and southern Pacific of Colombia. We genotyped 15 microsatellite loci, sexed all samples, and performed genotype comparisons. Genotype comparisons were done using a DNA register of previously genotyped individuals from the Colombian breeding ground and the Antarctic Peninsula feeding Area. We confirmed connectivity between feeding grounds in Antarctic Peninsula and breeding grounds in Colombia and Ecuador using microsatellite loci. We found recaptures within each sampling location in the same year, but we also found a few recaptures in the same locations between years both in breeding and feeding grounds....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Megaptera novaeangliae; Southeastern Pacific; Genotyping; Microsatellite; Migratory connectivity..
Ano: 2021 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612021000300031
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Utilization of microsatellites for the analysis of genomic alterations in colorectal cancers in Brazil BJMBR
Fuzikawa,A.K.; Haddad,L.A.; da-Cunha-Melo,J.R.; Brasileiro-Filho,G.; Pena,S.D.J..
Two different pathogenetic mechanisms are proposed for colorectal cancers. One, the so-called "classic pathway", is the most common and depends on multiple additive mutational events (germline and/or somatic) in tumor suppressor genes and oncogenes, frequently involving chromosomal deletions in key genomic regions. Methodologically this pathway is recognizable by the phenomenon of loss of heterozygosity. On the other hand, the "mutator pathway" depends on early mutational loss of the mismatch repair system (germline and/or somatic) leading to accelerated accumulation of gene mutations in critical target genes and progression to malignancy. Methodologically this second pathway is recognizable by the phenomenon of microsatellite instability. The distinction...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Polymerase chain reaction; Colorectal carcinoma; Microsatellite; P53; DCC; Microsatellite instability.
Ano: 1997 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000800001
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Genetic variability within Fusarium solani specie as revealed by PCR-fingerprinting based on pcr markers BJM
Brasileiro,Bereneuza Tavares Ramos Valente; Coimbra,Maria Raquel Moura; Morais Jr,Marcos Antonio de; Oliveira,Neiva Tinti de.
Fusarium solani fungus (teleomorph Haematonectria haematococca) is of relevance for agriculture, producing a disease that causes significant losses for many cultivars. Moreover, F. solani is an opportunistic pathogen to animals and humans. The complexity associated to its correct identification by traditional methods justifies the efforts of using molecular markers for isolates characterization. In this work, three PCR-based methods (one PCR-ribotyping and two PCR-fingerprinting) were used to investigate the molecular variability of eighteen F. solani isolates from four Brazilian States, collected from different substrates. Genetic analysis revealed the intraspecific variability within the F. solani isolates, without any correlation to their geographical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Intron splice site; Fusarium solani; Microsatellite; PCR-fingerprinting; Ribosomal DNA.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200006
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Bioethanol strains of Saccharomyces cerevisiae characterised by microsatellite and stress resistance BJM
Reis,Vanda Renata; Antonangelo,Ana Teresa Burlamaqui Faraco; Bassi,Ana Paula Guarnieri; Colombi,Débora; Ceccato-Antonini,Sandra Regina.
Abstract Strains of Saccharomyces cerevisiae may display characteristics that are typical of rough-type colonies, made up of cells clustered in pseudohyphal structures and comprised of daughter buds that do not separate from the mother cell post-mitosis. These strains are known to occur frequently in fermentation tanks with significant lower ethanol yield when compared to fermentations carried out by smooth strains of S. cerevisiae that are composed of dispersed cells. In an attempt to delineate genetic and phenotypic differences underlying the two phenotypes, this study analysed 10 microsatellite loci of 22 S. cerevisiae strains as well as stress resistance towards high concentrations of ethanol and glucose, low pH and cell sedimentation rates. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fermentation; Stresses; Yeasts; Microsatellite; Contaminants.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200268
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Genetic Structure and Diversity of Four Chilean Sheep Breeds Chilean J. Agric. Res.
de la Barra,Rodrigo; Uribe,Héctor; Latorre,Etel; San Primitivo,Fermín; Arranz,Juan.
Chilean sheep breeds have a significant founder effect since they descend from very few parents, in some cases population inbreeding has increased and in others there have been significant differentiation processes as compared to their original population. The objective of this study was to estimate the current status of genetic diversity in sheep herds, which comprise the main sheep breeds in Chile, using molecular methods. Animals from four breeding herds were sampled and analyzed. The breeds studied were Corriedale, Suffolk Down, Romney Marsh and Chilota, these are the predominant numerical pure breeds in Chile. From each breed population 40 animals were sampled. Genetic characterization was done using nine microsatellite molecular markers (SSR)...
Tipo: Journal article Palavras-chave: Microsatellite; Diversity; Sheep; Chile.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000400016
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Analysis of genetic diversity in Argentinian heterotic maize populations using molecular markers Ciencia e Investigación Agraria
Morales,Marcelo; Decker,Viviana; Ornella,Leonardo.
Over the past three decades, traditional Argentinean Orange Flint maize cultivars have been replaced by the higher yielding U.S. Yellow Dent germplasms. However, fint cultivars are potentially resistant to biotic and/or abiotic stress. Thus, knowledge of genetic diversity and relationships among fint inbred lines would help reduce genetic vulnerability and broaden the genetic base of crops in national improvement programs. In this study, we report the analysis of 25 inbred Orange Flint germplasms and one dent using 21 microsatellite markers or Simple Sequence Repeats (SSR). The aim was to assess genetic diversity among these accessions and evaluate the usefulness of SSR markers for defning heterotic groups in temperate germplasm. Genetic diversity values...
Tipo: Journal article Palavras-chave: Cluster analysis; Microsatellite; Zea mays.
Ano: 2010 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000100015
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Genetics of two marine shrimp hatcheries of the Pacific white shrimp Litopenaeus vannamei (Boone, 1931) in Pernambuco, Brazil Ciência Rural
Lima,Ana Patrícia Souza de; Silva,Suzianny Maria Bezerra Cabral da; Oliveira,Karine Kelly Cavalcanti; Maggioni,Rodrigo; Coimbra,Maria Raquel Moura.
The shrimp industry has grown significantly over the past 10 years in Brazil, especially the farmed production of the exotic Pacific white shrimp, Litopenaeus vannamei. In 2004, this industry was marked by a productivity crisis, which stirred interest towards genetic improvement of shrimp stocks. Shrimp breeders importation was banned in Brazil by a govern Normative Instruction in 1997, as a sanitary precaution. Since then, broodstock replacement in hatcheries has been based on domestic stocks, raising concerns on the decline of genetic diversity and if the existing diversity would allow effective genetic improvement programs. In the present research, genetic parameters such as number of alleles, effective allele number, expected and observed...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Litopenaeus vannamei; Population genetics; Genetic diversity; Microsatellite.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000200011
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Genotypic characterization of ten microsatellite loci in two Brazilian free range (Caipira) chicken lines Ciência Rural
Possamai,Mari Helen Pagani; Battilana,Jaqueline; Paludo,Ediane; Herkenhoff,Marcos Edgar; Pértile,Fábio; Lima-Rosa,Carlos André da Veiga.
This study aimed to investigate the genetic variability of two Brazilian free range (Caipira) chickens lines using microsatellites analysis of ten loci. It was collected a total of 99 blood samples, which 49 were from Paraíso Pedrês (PP) and 50 were from Rubro Negra (RN) lines. The amplification of the DNA fragments was performed by polymerase chain reaction (PCR) and the genotyping was conduct using ABI 3130 sequencer. The allele number variation was among 3 (LEI0254) to 32 (LEI0212) in the PP line, and 4 (LEI0254) to 31 (LEI0212) in the RN line. The allelic average per locus was 13.3 and 13.1 in the PP and RN lines, respectively. The average observed and the expected heterozygosity were 0.650 and 0.820 in the PP line, and 0.671 and 0.804 in the RN line....
Tipo: Info:eu-repo/semantics/article Palavras-chave: (Caipira) broiler line; (Caipira) layer line; Microsatellite; Polymorphism.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000500877
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Variabilidad genética del mildiu (Bremia lactucae Regel) en Salamanca y San Miguel de Allende, Guanajuato, mediante RAPD e ISSR Colegio de Postgraduados
Martínez Martínez, Susana.
Bremia lactucae Regel causa una de las enfermedades de mayor importancia en la lechuga a nivel mundial. Debido a que este pseudohongo presenta dos tipos de reproducción: sexual y asexual, su diversidad genética es amplia y puede dar origen a nuevas razas. Dicha situación se desconoce en México, por lo que es importante conocer este aspecto en las zonas productoras de esta hortaliza, así como también la influencia que ejercen los factores ambientales sobre la incidencia y severidad del patógeno. Por ello, fueron determinadas la incidencia y severidad de B. lactucae en 15 variedades de lechuga, además se registraron variables ambientales como la temperatura, humedad relativa, humedad de la hoja y precipitación en los municipios de Salamanca y San Miguel de...
Tipo: Tesis Palavras-chave: Bremia lactucae; Mildiu; Diversidad genética; Microsatélites; Maestría; Fitopatología; Mildew; Genetic diversity; Microsatellite.
Ano: 2008 URL: http://hdl.handle.net/10521/1675
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Assessment of genetic structure in Mexican charolais herds using microsatellite markers Electron. J. Biotechnol.
Sifuentes-Rincón,Ana María; Puentes-Montiel,Herlinda; Parra-Bracamonte,Gaspar Manuel.
Knowledge of livestock genetic diversity is an essential step to respond to commercial demands and reach production objectives in different environments and production systems. The evaluation of animal genetic diversity is achieved by using molecular markers. Microsatellites are the most used markers for studies of this type. Eleven microsatellites were used to evaluate the genetic variation from three populations of Charolais cattle located in northeast Mexico. The studied populations exhibited a high allelic variability with a mean heterozygosity of 0.5. A moderate genetic differentiation between the Charolais populations (F ST = 0.079; P < 0.001) was observed. This suggests subdivisions in Charolais breed established in Mexico, due to genetic...
Tipo: Journal article Palavras-chave: Charolais; Genetic diversity; Microsatellite.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400002
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A simple ethanol wash of the tissue homogenates recovers high-quality genomic DNA from Corchorus species characterized by highly acidic and proteinaceous mucilages Electron. J. Biotechnol.
Kundu,Avijit; Sarkar,Debabrata; Bhattacharjee,Amit; Topdar,Niladri; Sinha,Mohit Kumar; Mahapatra,Bikash Sinha.
A simple miniprep based on early elimination of highly acidic and proteinaceous mucilages through ethanol washing of the tissue homogenates has been developed for the extraction of genomic DNA from mature leaves and seeds of Corchorus spp. As compared to high cetyltrimethylammonium bromide (CTAB)-NaCl DNA extraction followed by ethanol-based removal of remnant mucilages from the DNA pellet, this simple miniprep consistently and reproducibly recovers high amounts of DNA with good spectral qualities at A260/A280 and A260/A230. The purified DNA is efficiently digested by restriction endonucleases, and is suitable for PCR amplification of nuclear microsatellites with expected allele sizes.
Tipo: Journal article Palavras-chave: Corchorus; DNA extraction; Microsatellite; Mucilage; PCR; Restriction digestion.
Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100010
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In silico characterization of microsatellites in Eucalyptus spp.: abundance, length variation and transposon associations Genet. Mol. Biol.
Rabello,Edenilson; Souza,Adriane Nunes de; Saito,Daniel; Tsai,Siu Mui.
This study assessed the abundance of microsatellites, or simple sequence repeats (SSR), in 19 Eucalyptus EST libraries from FORESTs, containing cDNA sequences from five species: E. grandis, E. globulus, E. saligna, E. urophylla and E. camaldulensis. Overall, a total of 11,534 SSRs and 8,447 SSR-containing sequences (25.5% of total ESTs) were identified, with an average of 1 SSR/2.5 kb when considering all motifs and 1 SSR/3.1 kb when mononucleotides were not included. Dimeric repeats were the most abundant (41.03%), followed by trimerics (36.11%) and monomerics (19.59%). The most frequent motifs were A/T (87.24%) for monomerics, AG/CT (94.44%) for dimerics, CCG/CGG (37.87%) for trimerics, AAGG/CCTT (18.75%) for tetramerics, AGAGG/CCTCT (14.04%) for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Eucalyptus; EST; Microsatellite; Simple sequence repeat (SSR); Molecular marker.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000400013
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Genetic diversity and population structure of the Guinea pig (Cavia porcellus, Rodentia, caviidae) in Colombia Genet. Mol. Biol.
Burgos-Paz,William; Cerón-Muñoz,Mario; Solarte-Portilla,Carlos.
The aim was to establish the genetic diversity and population structure of three guinea pig lines, from seven production zones located in Nariño, southwest Colombia. A total of 384 individuals were genotyped with six microsatellite markers. The measurement of intrapopulation diversity revealed allelic richness ranging from 3.0 to 6.56, and observed heterozygosity (Ho) from 0.33 to 0.60, with a deficit in heterozygous individuals. Although statistically significant (p < 0.05), genetic differentiation between population pairs was found to be low. Genetic distance, as well as clustering of guinea-pig lines and populations, coincided with the historical and geographical distribution of the populations. Likewise, high genetic identity between improved and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Food security; Microsatellite; Population structure.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400027
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Genetic diversity of Morato's Digger Toad, Proceratophrys moratoi: spatial structure, gene flow, effective size and the need for differential management strategies of populations Genet. Mol. Biol.
Arruda,Mauricio P.; Costa,William P.; Recco-Pimentel,Shirlei M..
Abstract The Morato's Digger Toad, Proceratophrys moratoi, is a critically endangered toad species with a marked population decline in southern Brazilian Cerrado. Despite this, new populations are being discovered, primarily in the northern part of the distribution range, which raises a number of questions with regard to the conservation status of the species. The present study analyzed the genetic diversity of the species based on microsatellite markers. Our findings permitted the identification of two distinct management units. We found profound genetic structuring between the southern populations, on the left margin of the Tietê River, and all other populations. A marked reduction was observed in the contemporary gene flow among the central populations...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Proceratophrys moratoi; Microsatellite; Population structure; Management unit; Conservation.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300502
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QTL affecting body weight in a candidate region of cattle chromosome 5 Genet. Mol. Biol.
Machado,Mariana B.B.; Alencar,Maurício M.; Pereira,Andréa P.; Oliveira,Henrique N.; Casas,Eduardo; Coutinho,Luis L.; Regitano,Luciana C.A..
The objective of this work was to identify QTLs for liveweight in a candidate region of bovine chromosome 5. Half-sib families from two lines, one traditional and the other new, of Canchim beef cattle (5/8 Charolais + 3/8 Zebu) were genotyped for four microsatellite markers, including the microsatellite in the IGF-1 (insulin-like growth factor-1) promoter region. Significant differences in allele distribution between the two lines were found for three markers. Interval mapping analyses in this region indicated the presence of a QTL controlling birth weight (p < 0.05) and of a QTL influencing breeding value for yearling weight (p < 0.01) in the newer line of the breed. The previously identified interaction between the IGF-1 genotype and genetic group...
Tipo: Info:eu-repo/semantics/article Palavras-chave: QTL; Growth traits; Microsatellite; Bovine.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300008
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Genetic diversity in section Rhizomatosae of the genus Arachis (Fabaceae) based on microsatellite markers Genet. Mol. Biol.
Angelici,Carla M.L.C.D.; Hoshino,Andrea Akemi; Nóbile,Paula Macedo; Palmieri,Dario Abel; Valls,José F. Montenegro; Gimenes,Marcos A.; Lopes,Catalina Romero.
The genus Arachis (Fabaceae) native to South America, contains 80 species divided into nine sections, three of which contain species of special economic importance such as the cultivated peanut (Arachis hypogaea), belonging to the section Arachis, and some perennial forage species from sections Caulorrhizae and Rhizomatosae. We used microsatellite markers to assay genetic variability among 77 accessions of four species from section Rhizomatosae, the diploid Arachis burkartii (2n = 2x = 20) and the tetraploid Arachis glabrata, Arachis pseudovillosa and Arachis nitida (2n = 4x = 40). A total of 249 alleles were found in the fifteen loci analyzed and a high degree of intra and interspecific polymorphism was detected. The lowest intraspecific variation...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Arachis; Genetic diversity; Microsatellite; Peanut; Rhizomatosae.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100016
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Microsatellite studies on an isolated population of African descent in the Brazilian state of Bahia Genet. Mol. Biol.
Barbosa,Ana Angélica Leal; Sousa,Sandra Mara Bispo; Abé-Sandes,Kiyoko; Alonso,Carlos Alberto; Schneider,Vicente; Costa,Denise C.C.; Cavalli,Iglenir João; Azevêdo,Eliane Elisa Souza.
The African descent population of the Bananal community in the Brazilian state of Bahia (BA) was characterized as a genetic isolate and analyzed for some short tandem repeat (STR) microsatellite autosomic polymorphic loci (CSF1PO, TH01, TPOX, F13A1, FESFPS and vWA). These genetic variants were further compared to data obtained from an urban sample from the town of Jequié (BA) regarding demographic and anthropogenetic aspects. The Bananal sample comprised 32 unrelated individuals whereas Jequié was represented by 76 individuals. The Bananal Negroid Phenotypic Index (NPI) was 0.98 and the Negroid Cultural Index (NCI) 0.24. Consanguineous marriages occurred at a frequency of 34.61% and the F value was 0.0126. All six loci studied were in Hardy-Weinberg...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Microsatellite; African-descendant isolate.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100004
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