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Rhizogenic Induction in Adult Juglans regia L. cv. Serr Tissue Induced by Indole Butyric Acid and Agrobacterium rhizogenes Chilean J. Agric. Res.
Sánchez-Olate,Manuel; Sáez,Patricia; Ríos,Darcy.
The in vitro introduction of adult walnut (Juglans regia L.) tissue represents an opportunity to clone elite genotypes whose selection occurs in advanced ontogenic states. With the purpose of developing a protocol to allow mass propagation of valuable genotypes from adult material, a comparison was made between two root induction systems of walnut microshoots of the fourth subculture of adult walnut tissue of an in vitro introduction program previously reinvigorated through traditional grafting. Rhizogenic induction by indole-3-butyric acid (IBA) and Agrobacterium rhizogenes was used. The rhizogenic process was analyzed in two phases for both auxinic (T1: 3 mg L-1 IBA; T2: 5 mg L-1 IBA) and A. rhizogenes inductions (T3: A-477; T4: A-478). The first phase...
Tipo: Journal article Palavras-chave: Rooting; Microshoots; Adult material.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200019
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Adventitious shoots from nodule cluster cultures of Vriesea reitzii: an endemic and endangered bromeliad from atlantic forest Ciência Rural
Rech Filho,Arlindo; Vesco,Lírio Luiz Dal; Guerra,Miguel Pedro.
The Atlantic Forest is a biome with megadiversity and a number of bromeliads take part of it. This is the case of Vriesea reitzii, an endemic bromeliad threatened with extinction. Tissue culture techniques are valuable tools for the mass propagation of bromeliads, thus reducing pressure in the natural habitat. The aim of the present work was to establish an in vitro protocol based on the induction and proliferation of nodule cluster cultures of this species. Plantlets maintained in MS liquid culture medium plus NAA (2µM) and BAP (4µM) had the basal regions of leaves excised and then inoculated in gelled with agar (7g L-1) MS culture medium plus with Dicamba (2.5; 5; 10; 20 e 30µM) and Kin (2µM) or free of plant growth regulators. Nodule cluster cultures...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bromeliads; Microshoots; In vitro regeneration; Conservation; Acclimatization.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000300043
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