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| Havlíčková,Lenka; Klíma,Miroslav; Přibylová,Marie; Hilgert-Delgado,Alois Antonín; Kučera,Vratislav; Čurn,Vladislav. |
| Background Microspore embryogenesis and cytoplasmic male sterility system (CMS) are two approaches widely exploited in Brassica napus breeding for production of homozygous doubled haploid (DH) lines and F1 hybrids respectively. Cytoplasmic male sterility system (CMS) is one of the most important pollination systems for hybrid seed production and utilisation of doubled haploid system to quickly prepare fully homozygous fertility restorer lines for CMS Ogu-INRA is very beneficial. Generally, only a small part of microspore-derived embryos is used for plant regeneration, without any knowledge about their properties. Therefore, the possibility of early detection of desirable genotypes bearing a single dominant nuclear fertility restorer (Rfo) gene, can double... |
| Tipo: Journal article |
Palavras-chave: Cytoplasmic male sterility; Microspore embryogenesis; Ogura CMS; Oilseed rape; Rfo gene. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100011 |
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| Rodrigues,Lia Rosane; Forte,Bianca de Camargo; Bodanese-Zanettini,Maria Helena. |
| In the last three decades, research on soybean microspore embryogenesis was restricted to anther culture, which presents limitations such as the small number of responsive microspores and the high embryogenic potential of sporophytic tissues. Therefore, a sequence of studies was performed to establish appropriate conditions for the isolation and culture of soybean microspores and pollen grains as an alternative to anther culture. First, a pollen and microspore isolation technique was developed using floral buds from four soybean cultivars (Bragg, IAS 5, MG/BR-46 Conquista and BRSMT Uirapuru). This technique allowed the establishment of cultures with satisfactory density and characteristics. Subsequently, different culture conditions were tested. Although... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Androgenesis; Glycine max; Microspore culture; Microspore embryogenesis; Microspore isolation; Soybean. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000500002 |
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