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Walter, Rafael; Carvalho, Virginia Silva; Generoso, Andressa Leal; Rodrigues, Rosana; Gravina, Geraldo de Amaral. |
The in vitro culture of embryos is an important technique to enable the rescue of embryos from incompatible crosses. Studies analyzing the factors that affect the in vitro culture of zygotic embryos are scarce. This study evaluated the effect of the genotype (Capsicum baccatum and C. frutescens), the composition of the culture medium (MS and ½ MS medium with different concentrations of sucrose, IAA and GA3) and the stage of development (globular, cordiform, torpedo and cotyledonary) in the in vitro culture of immature embryos. Embryo germination was influenced primarily by the stage of development of the embryo and the composition of the culture medium. Regardless of the species, the most suitable culture medium for the germination of globular and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rescue embryo; Peppers; Embryo stage; Sucrose; Mineral salts.. |
Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39474 |
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Walter, Rafael; Carvalho, Virginia Silva; Generoso, Andressa Leal; Rodrigues, Rosana; Gravina, Geraldo de Amaral. |
The in vitro culture of embryos is an important technique to enable the rescue of embryos from incompatible crosses. Studies analyzing the factors that affect the in vitro culture of zygotic embryos are scarce. This study evaluated the effect of the genotype (Capsicum baccatum and C. frutescens), the composition of the culture medium (MS and ½ MS medium with different concentrations of sucrose, IAA and GA3) and the stage of development (globular, cordiform, torpedo and cotyledonary) in the in vitro culture of immature embryos. Embryo germination was influenced primarily by the stage of development of the embryo and the composition of the culture medium. Regardless of the species, the most suitable culture medium for the germination of globular and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rescue embryo; Peppers; Embryo stage; Sucrose; Mineral salts.. |
Ano: 2018 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/39474 |
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