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	Provedor de dados Ciênc. Tecnol. Aliment. (1) ArchiMer (1) Autor Araújo,Edílson Divino de (1) Derelle, Evelyne (1) Galand, Pierre E. (1) Jain,Sona Arun (1) Jesus,Flávia Thalita de (1) Le Bris, Nadine (1) Marchioro,Giulia Manso (1) Peru, Erwan (1) Puisay, Antoine (1) Rzeznik-orignac, Jadwiga (1) Mais... Palavra-chave Molecular approach (2) 16S (1) 18S (1) Bacteria (1) Botanical origin (1) Canyon Lacaze-Duthiers (1) Honey pollen (1) Marine biodiversity (1) Mediterranean Sea (1) Meiofauna (1) Metabarcording (1) Nematodes (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Text (1) Ano 2018 (1) 2013 (1) País Brazil (1) France (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Extraction of DNA from honey and its amplification by PCR for botanical identification Ciênc. Tecnol. Aliment. Jain,Sona Arun; Jesus,Flávia Thalita de; Marchioro,Giulia Manso; Araújo,Edílson Divino de. The physiochemical and biological properties of honey are directly associated to its floral origin. Some current commonly used methods for identification of botanical origin of honey involve palynological analysis, chromatographic methods, or direct observation of the bee behavior. However, these methods can be less sensitive and time consuming. DNA-based methods have become popular due to their simplicity, quickness, and reliability. The main objective of this research is to introduce a protocol for the extraction of DNA from honey and demonstrate that the molecular analysis of the extracted DNA can be used for its botanical identification. The original CTAB-based protocol for the extraction of DNA from plants was modified and used in the DNA extraction... Tipo: Info:eu-repo/semantics/article Palavras-chave: Botanical origin; Honey pollen; Molecular approach. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000400022 Co-occurring nematodes and bacteria in submarine canyon sediments ArchiMer Rzeznik-orignac, Jadwiga; Puisay, Antoine; Derelle, Evelyne; Peru, Erwan; Le Bris, Nadine; Galand, Pierre E.. In submarine canyon sediments, bacteria and nematodes dominate the benthic biomass and play a key role in nutrient cycling and energy transfer. The diversity of these communities remains, however, poorly studied. This work aims at describing the composition of bacteria and nematode communities in the Lacaze-Duthiers submarine canyon in the north-western Mediterranean Sea. We targeted three sediment depths for two consecutive years and investigated the communities using nuclear markers (18S rRNA and 16S rRNA genes). High throughput sequencing combined to maximal information coefficient (MIC) statistical analysis allowed us to identify, for the first time, at the same small scale, the community structures and the co-occurrence of nematodes and bacteria... Tipo: Text Palavras-chave: Bacteria; Meiofauna; Canyon Lacaze-Duthiers; Metabarcording; Molecular approach; Mediterranean Sea; Nematodes; Marine biodiversity; 18S; 16S. Ano: 2018 URL: https://archimer.ifremer.fr/doc/00454/56586/76447.pdf Registros recuperados: 2 Primeira ... 1 ... Última

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