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| Registros recuperados: 30 | |
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| GONÇALVES,André Luiz; CASTRO,Edilene Alcântara de; LUZ,Ennio; Cancio FENDRICH,Ricardo; Araújo de SOUZA,Nataly; THOMAZ-SOCCOL,Vanete. |
| ABSTRACT Lutzomyia intermedia (Diptera: Psychodidae) features as one of the main vectors that are involved in the transmission of American cutaneous leishmaniasis (ACL) in the Neotropical region. However, genetic studies involving this taxon are still incipient and important for understanding the level of variability of different populations, their role, and implications as vectors. The aim of this study was to determine the level of genetic diversity of L. intermedia present in the Ribeira River Valley, an area of ACL transmission in the state of Paraná, Brazil, through the Random amplified polymorphic DNA (RAPD). Two municipalities were chosen to collect sand flies: Cerro Azul (new transmission area of the ACL) and Adrianópolis (endemic area of the ACL).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Intraspecific variation; Molecular epidemiology; Phenetic analysis; Phlebotominae. |
| Ano: 2021 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2021000300365 |
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| Martínez,Ana M.B.; Hora,Vanusa P. da; Santos,Adriana L. dos; Mendoza-Sassi,Raul; Von Groll,Andréa; Soares,Esmeralda A.J.M.; D'Ávila,Nildo; Silveira,Jussara; Leal,Renata G.; Tanuri,Amilcar; Soares,Marcelo A.. |
| Different human immunodeficiency virus type 1 (HIV-1) subtypes may have distinct biological, immunological and pathogenic properties. Efficiency of mother-to-child transmission (MTCT) may be among those properties, but few and controversial results have been described so far. In this study, 102 children born from HIV-1-infected mothers between 1998 and 2004 in the city of Rio Grande, Brazil were analyzed for potential risk factors associated with MTCT. That geographic region is characterized by a high proportion of subtype C-infected subjects, and it allowed comparison between subtypes B and C and their influence on MTCT. The analysis also included clinical, obstetric and immunological parameters. Multivariate regression analyses were conducted to evaluate... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV; MTCT; Vertical transmission; Subtype C; Molecular epidemiology. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652006000100011 |
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| Duarte,S.C.; Parente,J.A.; Silveira Neto,O.J.; Jayme,V.S.; Bastos,T.S.A.; Linhares,G.F.C.. |
| ABSTRACT More than 300 species have been described in the genus Hepatozoon, occurring in different vertebrates. Among these, only Hepatozoon canis and Hepatozoon americanum are seen in dogs. Different methods may be used for laboratory diagnosis. The most common of these is direct parasitological examination of parasite stages in blood smears. The aim of this investigation was to conduct a phylogenetic study on Hepatozoon isolates from symptomatic dogs in the city of Goiânia, Goiás, Brazil. Blood samples were obtained from 40 symptomatic dogs that had been referred to the Veterinary Hospital of the Federal University of Goiás. Among these, only two samples were positive for Hepatozoon spp. using the direct parasitological method. These samples were then... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Canis familiaris; Hepatozoonosis; Molecular epidemiology; Phylogeny. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000601431 |
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| Zhao,Ting; Ye,Zhixu; Wang,Binlin; Cui,Yuxia; Nie,Yingjie; Yang,Bin; Chen,Kun; Zhang,Hua; Hu,Fangfang; Yu,Fuxun. |
| ABSTRACT To investigate the genetic variation and molecular epidemiology characteristics of Human Respiratory Syncytial Virus (HRSV) in Guizhou Province, nasopharyngeal aspirates were collected from patients with acute respiratory infection (ARI) in Guizhou Provincial People's Hospital, from December 2017 to March 2018, and inoculated to Hep-2 cells to isolate HRSV. Cells that showed cytopathic effect (CPE) were then confirmed by indirect immunofluorescence assay and reverse transcription. The sequence of the PCR products was determined for HRSV isolates, and the genetic variation was analyzed. Out of 196 nasopharyngeal aspirate samples, HRSV were isolated in 39. The second hypervariable region at the 3' terminal of glycoprotein gene (HVR2) sequence... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Human respiratory syncytial virus; Virus isolation; RT-PCR; HVR2; Phylogenetic analysis; Molecular epidemiology. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702019000600427 |
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| Castro,Lisie Souza; Rezende,Grazielli Rocha de; Fernandes,Fernanda Rodas Pires; Bandeira,Larissa Melo; Puga,Marco Antonio Moreira; Tanaka,Tayana Serpa Ortiz; Weis-Torres,Sabrina Moreira dos Santos; Vicente,Ana Carolina Paulo; Otsuki,Koko; Motta-Castro,Ana Rita Coimbra. |
| ABSTRACT Human T cell lymphotropic virus type 1 (HTLV-1) was the first retrovirus discovered in humans and is endemic in several parts of the world. Because of risk behaviors, mainly sexual, men who have sex with men (MSM) are at high risk of acquiring HTLV-1 infection. A cross-sectional study was performed to estimate the prevalence of HTLV-1 infection, to characterize genetically HTLV-1 sequences and to identify risk behaviors associated with this infection among MSM in Central Brazil. A total of 430 MSM were enrolled in this study and three were shown to be HTLV-1 infected, prevalence of 0.7% (95% confidence interval: 0.4-0.9). Phylogenetic analysis showed that all HTLV-1 positive samples belonged to Cosmopolitan subtype Transcontinental subgroup A.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HTLV-1; Men who have sex with men; Sexual risk behavior; Molecular epidemiology; Brazil. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000600472 |
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| Brito,Denise von Dolinger de; Oliveira,Elias José; Abdallah,Vânia O. Steffen; Darini,Ana Lúcia da Costa; Gontijo Filho,Paulo P.. |
| We studied an outbreak of two multi-drug resistant clones of Acinetobacter baumannii in the Neonatal Intensive Care Unit of the Uberlândia Federal University Hospital in Minas Gerais state, Brazil, and we analyzed the contribution of cross-transmission in the rise in infection rates. Eleven neonates who developed multi-drug resistant A. baumannii nosocomial infection were matched to 22 neonates who were admitted to the same unit and did not develop an infection during the outbreak period, in order to identify risk factors for infection. Three out of the 11 neonates died. Epidemiological investigation included molecular typing, using pulsed field gel electrophoresis. Prior to the outbreak, from December 2001 to March 2002, no case of infection by this... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acinetobacter baumannii; Molecular epidemiology; Outbreak; Neonates. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702005000400006 |
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| Fahl,Willian Oliveira; Carnieli Jr,Pedro; Castilho,Juliana Galera; Carrieri,Maria Luiza; Kotait,Ivanete; Iamamoto,Keila; Oliveira,Rafael Novaes; Brandão,Paulo Eduardo. |
| In Brazil, bats have been assigned an increasing importance in public health as they are important rabies reservoirs. Phylogenetic studies have shown that rabies virus (RABV) strains from frugivorous bats Artibeus spp. are closely associated to those from the vampire bat Desmodus rotundus, but little is known about the molecular diversity of RABV in Artibeus spp. The N and G genes of RABV isolated from Artibeus spp. and cattle infected by D. rotundus were sequenced, and phylogenetic trees were constructed. The N gene nucleotides tree showed three clusters: one for D. rotundus and two for Artibeus spp. Regarding putative N amino acid-trees, two clusters were formed, one for D. rotundus and another for Artibeus spp. RABV G gene phylogeny supported the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rabies Bat Artibeus spp.; Desmodus rotundus; Phylogeny; Molecular epidemiology. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600008 |
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| Royer,Sabrina; Faria,Ana Luiza Souza; Seki,Liliane Miyuki; Chagas,Thiago Pavoni Gomes; Campos,Paola Amaral de; Batistão,Deivid William da Fonseca; Asensi,Marise Dutra; Gontijo Filho,Paulo P.; Ribas,Rosineide Marques. |
| Background:In Brazil, ventilator-associated pneumonia (VAP) caused by carbapenem resis- tant Acinetobacter baumanniiand Pseudomonas aeruginosaisolates are associated with significant mortality, morbidity and costs. Studies on the clonal relatedness of these isolates could lay the foundation for effective infection prevention and control programs.Objectives: We sought to study the epidemiological and molecular characteristics of A. baumannii vs. P. aeruginosaVAP in an adult intensive care unit (ICU).Methods: It was conducted a cohort study of patients with VAP caused by carbapenem resistant A. baumanniiand P'. aeruginosaduring 14 months in an adult ICU. Genomic studies were used to investigate the clonal relatedness of carbapenem resistant OXA-23-producing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acinetobacter baumannii; Intensive care unit; Molecular epidemiology; Multidrug-resistant. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000400350 |
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| Rodríguez-Noriega,Eduardo; Seas,Carlos. |
| Methicillin-resistant Staphylococcus aureus (MRSA) clones belonging to the Brazilian, Pediatric, Cordobes/Chilean and New York/Japan clonal complexes are widely distributed across Latin America, although their individual distribution patterns and resistance to antimicrobial drugs are constantly changing. Furthermore, clones with increased virulence are beginning to appear more frequently both in hospital and community settings, and there is evidence that virulence factors can be transferred between hospital- and community-associated clones through recombination. These changing patterns have significant implications for clinical practice in the region. Most importantly, clinicians need to be aware of the changing antimicrobial resistance profile of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MRSA; Clones; Molecular epidemiology; Latin America. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000800004 |
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| Rossini,M.A.A.; Diaz,R.S.; Caseiro,M.; Turcato,G.; Accetturi,C.A.; Sabino,E.C.. |
| In order to assess the molecular epidemiology of HIV-1 in two neighboring cities located near the epicenter of the HIV-1 epidemics in Brazil (Santos and São Paulo), we investigated 83 HIV-1 strains obtained from samples collected in 1995 from intravenous drug users. The V3 through V5 region of the envelope of gp 120 was analyzed by heteroduplex mobility analysis. Of the 95 samples, 12 (12.6%) were PCR negative (6 samples from each group); low DNA concentration was the reason for non-amplification in half of these cases. Of the 42 typed cases from São Paulo, 34 (81%, 95% confidence limits 74.9 to 87.0%) were B and 8 (19%, 95% confidence limits 12.9 to 25.0%) were F, whereas of the 41 typed cases from Santos, 39 (95%, 95% confidence limits 91.6 to 98.4%)... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: HIV-1 subtypes; Intravenous drug users; Molecular epidemiology; Heteroduplex mobility analysis; Brazil. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100005 |
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| Beretta,A.L.R.Z.; Trabasso,P.; Stucchi,R.B.; Moretti,M.L.. |
| Methicillin-resistant Staphylococcus aureus (MRSA) has been the cause of major outbreaks and epidemics among hospitalized patients, with high mortality and morbidity rates. We studied the genomic diversity of MRSA strains isolated from patients with nosocomial infection in a University Hospital from 1991 to 2001. The study consisted of two periods: period I, from 1991 to 1993 and period II from 1995 to 2001. DNA was typed by pulsed-field gel electrophoresis and the similarity among the MRSA strains was determined by cluster analysis. During period I, 73 strains presented five distinctive DNA profiles: A, B, C, D, and E. Profile A was the most frequent DNA pattern and was identified in 55 (75.3%) strains; three closely related and four possibly related... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Methicillin-resistant Staphylococcus aureus; Molecular epidemiology; Hospital infection; Pulsed-field gel electrophoresis. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000900009 |
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| Wünsch-Filho,V.; Eluf-Neto,J.; Lotufo,P.A.; da Silva Jr.,W.A.; Zago,M.A.. |
| Genomics is expanding the horizons of epidemiology, providing a new dimension for classical epidemiological studies and inspiring the development of large-scale multicenter studies with the statistical power necessary for the assessment of gene-gene and gene-environment interactions in cancer etiology and prognosis. This paper describes the methodology of the Clinical Genome of Cancer Project in São Paulo, Brazil (CGCP), which includes patients with nine types of tumors and controls. Three major epidemiological designs were used to reach specific objectives: cross-sectional studies to examine gene expression, case-control studies to evaluate etiological factors, and follow-up studies to analyze genetic profiles in prognosis. The clinical groups included... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Multicenter studies; Large-scale studies; Molecular epidemiology; Data control quality; Cancer epidemiological studies. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400016 |
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| Villar,L.M.; Morais,L.M.; Aloise,R.; Melo,M.M.M.; Calado,I.A.; Lampe,E.; Gaspar,A.M.C.. |
| The Northeast region is the location of most cases of acute hepatitis A virus (HAV) in Brazil. In the present study, the genotypes of HAV strains from Pernambuco State, one of most populous states in the Northeast region, were characterized. Blood samples positive for anti-HAV IgM from 145 individuals (mean age = 29.1 years), collected during 2002 and 2003, were submitted to nested RT-PCR for amplification of the 5'non-translated region (5'NTR) and VP1/2A regions of the HAV genome. The VP1/2A and 5'NTR regions were amplified in 39 and 21% of the samples, respectively. Nucleotide sequencing was carried out in 46% of VP1/2A and in 53% of 5'NTR isolates. The identity in nucleotide sequence of the VP1/2A region ranged from 93.6 to 100.0%. Phylogenetic analysis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hepatitis A virus; Molecular epidemiology; Sub-genotype IA; Sub-genotype IB. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000700004 |
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| Entiauspe,Ludmila G; Silveira,Mariângela; Nunes,Emily M; Basgalupp,Suélen P; Stauffert,Dulce; Dellagostini,Odir A.; Collares,Tiago; Seixas,Fabiana K.. |
| Oncogenic HPV genotypes are strongly associated with premalignant and malignant cervical lesion. The purpose was to determine human papillomavirus (HPV) prevalence and genotypes, and to estimate cervical cancer risk factor associations. Cervical samples were obtained from 251 women seeking gynecological care at the Pelotas School of Medicine Clinic. This is a cross-sectional study. HPV-DNA was amplified by nested-PCR using MY09/11 and GP5/6 primers, and the sequencing was used for genotyping. Sociodemographic and behavioral risk factors were obtained by closed questionnaire, and its relationship to HPV infection prevalence were analyzed. Statistical analyses were performed using SPSS 16.0 software, and differences were considered significant at p <... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HPV; SDT; Molecular epidemiology; Cervical cancer. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200043 |
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| Arslan,Serdal; Degerli,Naci; Bardakci,Fevzi. |
| NAT1 is an intronless gene on chromosome 8p21.3 encoding a 290-amino-acid-long protein showing acetyltransferase activity. Some 26 alleles of NAT1 gene have been identified in human populations. In the present study we determined the distributions of NAT1 genotypes and alleles in a sample of 201 individuals from the Turkish population in Central Anatolia. The most frequent genotypes were NAT1*4/NAT1*4 (51.74%), NAT1*10/NAT1*4 (22.39%), NAT1*11/NAT1*4 (7.46), NAT1*10/NAT1*10 (3.98%). Frequencies of NAT1*3, *4 (wild-type), *10 and *11 alleles were 3.73%, 69.6%, 17.66% and 7.2%, respectively. The frequency of NAT1*11 was the highest amongst the populations studied so far, the other allele frequencies being close to those described in Caucasian populations. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: NAT1 gene; Genetic polymorphism; Molecular epidemiology; Turkish population. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200005 |
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| Registros recuperados: 30 | |
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