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Expression of the antibody 14D9 in Nicotiana tabacum hairy roots Electron. J. Biotechnol.
Martínez,Carolina; Petruccelli,Silvana; Giulietti,Ana María; Alvarez,María Alejandra.
Nicotiana tabacum hairy roots that express the antibody 14D9 were established. The 14D9 antibody yield obtained after 20 days of culture was 5.95 μg 14D9ml-1. The addition of the reticulum endoplasmic retention sequence KDEL demonstrated a positive effect over the intracellular 14D9 amounts with a yield increase up to 20.82 µg ml-1. DMSO increased the antibody amount in the biomass from 20.00 to 64.03 µg ml-1 while PVP (at 1.5 gl-1) and gelatine (at 5.0 gl-1) increased total 14D9 amounts in the culture medium to 25 µg and 14 µg respectively
Tipo: Journal article Palavras-chave: Catalytic antibodies; In vitro cultures; Molecular farming; Recombinant antibodies; Tobacco.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200006
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Pharmaceutical and Industrial Traits in Genetically Modified Crops: Co-existence with Conventional Agriculture AgEcon
Moschini, GianCarlo.
This paper discusses the implications of using genetically modified crops to biomanufacture pharmaceuticals and industrial compounds from the perspective of their co-existence with conventional agriculture. Such plant-made pharmaceuticals and plant-made industrial products rely on exciting scientific and technological breakthroughs and promise new opportunities for the agricultural sector, but they also entail novel risks. The management of the externalities and of the possible unintended economic effects that arise in this context is critical and poses difficult questions for regulators.
Tipo: Working or Discussion Paper Palavras-chave: Agriculture; Biopharming; Co-existence; Externalities; Genetically modified products; Liability; Molecular farming; Regulation; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 2006 URL: http://purl.umn.edu/18544
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