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	Provedor de dados 114 (1) 58 (1) 80 (1) Autor Andreote,Fernando Dini (1) Anschau,ME (1) Araújo,Welington Luiz (1) Asinari,F (1) Azevedo,João Lúcio (1) Borges,ALP (1) Cafrune,E.E (1) Castelan,LA (1) Conci,L.R (1) Conci,V.C (1) Guzmán,F.A (1) Rodrigues,MT (1) Spalding,SM (1) Staggemeier,R (1) Tavares,RG (1) Vasconcelos,J (1) Mais... Palavra-chave Molecular techniques (3) Diagnosis (1) Endophytes (1) Fragaria x ananassa (1) Molecular epidemiology (1) Multivariate analysis (1) Parasite infection (1) Plant-bacteria interactions (1) Rhizosphere (1) Screening viruses (1) Virus diagnosis (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2016 (1) 2011 (1) 2009 (1) País Brazil (2) Argentina (1) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Development of a non-radioactive molecular hybridization probe for detecting Strawberry mottle virus in strawberry 114 Asinari,F; Cafrune,E.E; Guzmán,F.A; Conci,L.R; Conci,V.C. The vegetative propagation of strawberries favors transmission of systemic pathogens, such as viruses, which are one of the main yield-limiting factors for this crop. More than 20 viruses have been described as infecting this species; one of the most frequent is the Strawberry mottle virus (SMoV), which is responsible for significant economic losses. SMoV is usually detected by reverse transcription polymerase chain reaction (RT-PCR), given that serum is not available for serological tests. In this study, a non-radioactive molecular probe was developed for SMoV detection. The cDNA was synthesized by RT-PCR using specific primers designed from the 3’UTR region of the viral genome. The cloned cDNA segment was labeled and used as a probe. Six RNA... Tipo: Info:eu-repo/semantics/article Palavras-chave: Fragaria x ananassa; Molecular techniques; Screening viruses; Virus diagnosis. Ano: 2016 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-298X2016000100004 Assessing the diversity of bacterial communities associated with plants 58 Andreote,Fernando Dini; Azevedo,João Lúcio; Araújo,Welington Luiz. Plant-bacteria interactions result from reciprocal recognition between both species. These interactions are responsible for essential biological processes in plant development and health status. Here, we present a review of the methodologies applied to investigate shifts in bacterial communities associated with plants. A description of techniques is made from initial isolations to culture-independent approaches focusing on quantitative Polymerase Chain Reaction in real time (qPCR), Denaturing Gradient Gel Electrophoresis (DGGE), clone library construction and analysis, the application of multivariate analyses to microbial ecology data and the upcoming high throughput methodologies such as microarrays and pyrosequencing. This review supplies information... Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant-bacteria interactions; Molecular techniques; Multivariate analysis; Endophytes; Rhizosphere. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300001 Molecular techniques for the study and diagnosis of parasite infection 80 Tavares,RG; Staggemeier,R; Borges,ALP; Rodrigues,MT; Castelan,LA; Vasconcelos,J; Anschau,ME; Spalding,SM. In parasitology, routine laboratory diagnosis involves conventional methods, such as optical microscopy, used for the morphological identification of parasites. Currently, molecular biology techniques are increasingly used to diagnose parasite structures in order to enhance the identification and characterization of parasites. The objective of the present study was to review the main current and new diagnostic techniques for confirmation of parasite infections, namely: polymerase chain reaction (PCR), real-time polymerase chain reaction (RT-PCR), loop-mediated isothermal amplification (LAMP), Luminex xMAP, random amplified polymorphic DNA (RAPD), amplified fragment length polymorphism (AFLP), and restriction fragment length polymorphism (RFLP), in addition... Tipo: Info:eu-repo/semantics/article Palavras-chave: Parasite infection; Diagnosis; Molecular techniques; Molecular epidemiology. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300003 Registros recuperados: 3 Primeira ... 1 ... Última

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