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| Registros recuperados: 12 | |
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| Sena,Amanda R.; Júnior,Gildomar L.V.; Góes Neto,Aristóteles; Taranto,Alex G.; Pirovani,Carlos P.; Cascardo,Júlio C.M.; Zingali,Russolina B.; Bezerra,Marcos A.; Assis,Sandra A.. |
| The enzyme glucanase from Moniliophthora perniciosa was produced in liquid medium and purified from the culture supernatant. A multivariate statistical approach (Response Surface Methodology - RSM) was employed to evaluate the effect of variables, including inducer (yeast extract) and fermentation time, on secreted glucanase activities M. perniciosa detected in the culture medium. The crude enzyme present in the supernatant was purified in two steps: precipitation with ammonium sulfate (70%) and gel filtration chromatography on Sephacryl S-200. The best inducer and fermentation time for glucanase activities were 5.9 g L-1 and 13 days, respectively. The results revealed three different isoforms (GLUI, GLUII and GLUIII) with purification factors of 4.33,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glucanase; Moniliophthora perniciosa; Production; Kinetic characterization; Purification; Heat stability. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652011000200019 |
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| Galante,Rafaela S.; Taranto,Alex G.; Koblitz,Maria G.B.; Góes-Neto,Aristóteles; Pirovani,Carlos P.; Cascardo,Júlio C.M.; Cruz,Sandra H.; Pereira,Gonçalo A.G.; Assis,Sandra A. de. |
| The enzyme chitinase from Moniliophthora perniciosa the causative agent of the witches' broom disease in Theobroma cacao, was partially purified with ammonium sulfate and filtration by Sephacryl S-200 using sodium phosphate as an extraction buffer. Response surface methodology (RSM) was used to determine the optimum pH and temperature conditions. Four different isoenzymes were obtained: ChitMp I, ChitMp II, ChitMp III and ChitMp IV. ChitMp I had an optimum temperature at 44-73ºC and an optimum pH at 7.0-8.4. ChitMp II had an optimum temperature at 45-73ºC and an optimum pH at 7.0-8.4. ChitMp III had an optimum temperature at 54-67ºC and an optimum pH at 7.3-8.8. ChitMp IV had an optimum temperature at 60ºC and an optimum pH at 7.0. For the computational... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chitinase; Moniliophthora perniciosa; Kinetic characterization; Purification; Isoenzymes; Heat stability; 3D structure; Comparative modeling. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652012000200016 |
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| Lima,Juliana O.; Pereira,Jorge F.; Rincones,Johana; Barau,Joan G.; Araújo,Elza F.; Pereira,Gonçalo A.G.; Queiroz,Marisa V.. |
| This report describes the cloning, sequence and expression analysis of the glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene of Moniliophthora perniciosa, the most important pathogen of cocoa in Brazil. Southern blot analysis revealed the presence of a single copy of the GAPDH gene in the M. perniciosa genome (MpGAPDH). The complete MpGAPDH coding sequence contained 1,461 bp with eight introns that were conserved in the GAPDH genes of other basidiomycete species. The cis-elements in the promoter region of the MpGAPDH gene were similar to those of other basidiomycetes. Likewise, the MpGAPDH gene encoded a putative 339 amino acid protein that shared significant sequence similarity with other GAPDH proteins in fungi, plants, and metazoans. Phylogenetic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glyceraldehyde-3-phosphate dehydrogenase gene; Moniliophthora perniciosa; Witches' broom. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200024 |
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| SOUZA, M. G. de; SOUZA, A. das G. C. de; ALMEIDA, O. C. de. |
| A vassoura de bruxa, doença endêmica da região amazônica, é considerada a mais prejudicial à cultura do cupuaçuzeiro. Em mudas atacadas, o sintoma mais frequente é a vassoura terminal (VT), pois o fungo infecta principalmente a gema apical. A medida mais segura e de baixo custo para o manejo da doença é o uso de cultivares resistentes a ela. Porém o ciclo no melhoramento genético da cultura é longo. Assim, o uso de metodologia que permita fazer comparações na fase de mudas é de grande valia para acelerar o processo de seleção e desenvolvimento de cultivares de cupuaçuzeiro resistentes à vassoura de bruxa, pois genótipos suscetíveis podem ser descartados precocemente. Neste contexto, objetivou-se avaliar a metodologia de inoculação artificial do fungo... |
| Tipo: Folhetos |
Palavras-chave: Vassoura-de-bruxa; Fruteira nativa; Inoculação Artificial; Doença de Planta; Cupuaçu; Moniliophthora perniciosa. |
| Ano: 2022 |
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1150413 |
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| Silva,Stela Dalva Vieira Midlej; Luz,Edna Dora Martins Newman; Pires,José Luis; Yamada,Milton Macoto; Santos Filho,Lindolfo Pereira dos. |
| The objective of this work was to identify genotypes with high general combining ability for resistance to witches'-broom (Moniliophthora perniciosa) in populations formed from a first cycle of recurrent selection. Highly productive and resistant clones from different origins were interbred using the North Carolina II design. The clones SCA 6, CSUL 7, RB 39, CEPEC 89, OC 67, BE 4, EEG 29 and ICS 98 were used as paternal parents, while the maternal ones were NA 33, CCN 10, IMC 67, P 4B, CCN 51, CEPEC 86, SGU 54 and ICS 9. Twenty days after germination, 56 seedlings of each cross (four replicates of 14 seedlings) received the inoculation of a 1-mL suspension with 7.5x10(4 ) basidiospores mL-1. Symptoms were evaluated 60 days after inoculation. Significant... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Moniliophthora perniciosa; Theobroma cacao; Disease integrate management; Early selection; Genetic resistance. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010000700007 |
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| Santos,Rogério Mercês Ferreira; Lopes,Uilson Vanderlei; Bahia,Rita de Cássia; Machado,Regina Celle Rebouças; Ahnert,Dário; Corrêa,Ronan Xavier. |
| Os objetivos deste trabalho foram caracterizar a resistência à vassoura-de-bruxa de plantas de cacau originadas do cruzamento entre TSH 1188 e CCN 51 (população segregante), por meio de dois métodos de inoculação em condições de campo, e identificar marcadores microssatélites específicos para grupos de plantas resistentes e suscetíveis. As plantas-controle avaliadas pelos métodos de inoculação natural e inoculação artificial em campo produziram os mesmos padrões de sintomas. As plantas da população segregante também coincidiram os padrões de sintomas em 90%, por esses dois métodos. O método de inoculação artificial em campo permite detectar falso-resistentes. Dos 18 pares de primers microssatélites amplificados, 15 foram polimórficos entre os genitores, e... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Theobroma cacao; Moniliophthora perniciosa; Inoculação; Marcador molecular; QTL; Melhoramento genético. |
| Ano: 2007 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000800010 |
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| Costa,Larissa Corrêa Bomfim; Pinto,José Eduardo Brasil Pereira; Bertolucci,Suzan Kelly Vilela; Costa,João Cássia do Bomfim; Alves,Péricles Barreto; Niculau,Edenilson dos Santos. |
| The efficacy of Ocimum selloi essential oil was evaluated for controlling the growth of mycelia and spores germination Moniliophthora perniciosa. Six compounds (99.89%) of the total oil were identified by GC-MS, of which methyl chavicol, methyl eugenol, β-caryophyllene, germacrene-D, bicyclogermacrene and spathulenol. Essential oil was tested for antifungal activity, which was determined by disc diffusion and minimum inhibitory concentration (MIC) determination methods. Application of the oil reduced mycelial growth in a dose dependent manner, with maximum inhibition being observed at concentration of 1,000 ppm. Such antifungal activity could be attributed to methyl chavicol since the pure compound was shown to be similarly effective against Moniliophthora... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antifungal Activity; Methyl Chavicol; Moniliophthora perniciosa; Theobroma cacao. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902015000200428 |
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| Registros recuperados: 12 | |
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