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| Registros recuperados: 69 | |
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| Souza,Allyson S. de; Dias Júnior,Eurico A.; Galetti Jr,Pedro M.; Machado,Erik G.; Pichorim,Mauro; Molina,Wagner F.. |
| The Epinephelidae form a group of species of high biological and economical interests. It´s phylogeographic patterns are not well known especially the distributed populations in the western region of the Atlantic Ocean. Among the representatives is a small species called Cephalopholis fulva, Coney, which presents a wide geographical distribution, polychromia, hermaphroditism and is quickly becoming a large target for the exploration of commercial fishing. The genetic and historical demography were obtained through the partial sequence analysis of Control Region from six locations on the coastline of Brazil from the northeast coast to the southwest coast, including the oceanic islands of Rocas Atoll and Fernando de Noronha Archipelago. The analyzed samples... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: D-loop; Glaciation; MtDNA; Panmixia; Phylogeography. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000100121 |
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| Lakra,Wazir S; Goswami,M; Rajaswaminathan,T; Rathore,Gaurav. |
| Two new cell lines (CCF and CCH) were established from fin and heart tissues of common carp, Cyprinus carpio. The cells were optimally maintained in Leibovitz-15 medium supplemented with 10% fetal bovine serum (FBS) and 10 ng/ml of basic fibroblastic growth factor (bFGF). The effects of temperature, concentration of FBS and bFGF on the growth of CCF and CCH cells were examined. The temperature ranged from 24 to 32 °C for good growth of the cells. The growth rate of cells was higher in medium containing 10% FBS and the addition of bFGF to the medium significantly increased the growth rate. The CCF cells were found to be epithelial, while the CCH cells were fibroblastic in nature. The cytogenetic analysis of the cell lines revealed a diploid number of 100... |
| Tipo: Journal article |
Palavras-chave: Cyprinus carpio; Cell line; MtDNA. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000400002 |
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| Hernández-Ardila,Laura Valentina; Barragán-Barrera,Dalia C.; Negrete,Javier; Poljak,Sebastián; Riet-Sapriza,Federico G.; Caballero,Susana. |
| ABSTRACT The leopard seal (Hydrurga leptonyx) is a genetically low-studied species. In order to conduct the first genetic diversity assessment of this species in Danco Coast (Antarctic Peninsula), 423 bp of the mitochondrial DNA Control Region (mtDNA-CR) was sequenced from 13 blood samples collected in Primavera Base (62º15’S, 58º39’W) during the 2011-2012 austral summer. Our results showed high haplotype diversity (h = 0.99), with various divergent haplotypes. Our findings suggest that leopard seals in the Danco Coast could represent different phylogroups; however, including more genetic markers are needed to confirm this hypothesis. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Seal; Pinnipeds; MtDNA; Control Region; Antarctica.. |
| Ano: 2021 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0122-97612021000300227 |
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| Panarari-Antunes,Renata de Souza; Prioli,Alberto José; Prioli,Sônia Maria Alves Pinto; Galdino,Alexsandro Sobreira; Julio Junior,Horácio Ferreira; Prioli,Laudenir Maria. |
| This study aimed to evaluate the genetic variability of B. orbignyanus in cultivated and natural populations of the Upper Paraná River, using molecular RAPD markers and mtDNA control region. Specimens were collected in the Paraná River and in the Piracema fish farm in Maringá, State of Paraná, Brazil. RAPD primers produced 82 loci with consistent expression. The population from the Paraná River showed 28 polymorphic loci, whereas the population from the fish farm presented only 12. Data revealed genetic differentiation between the two populations, although not very pronounced. These results were corroborated by the principal coordinate analysis and by neighbor-joining clustering. The alignment of the D-loop sequences of B. orbignyanus indicated the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Freshwater fish; Endangered; RAPD; MtDNA; Polymorphism. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000400025 |
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| Artico,L.O.; Bianchini,A.; Grubel,K.S.; Monteiro,D.S.; Estima,S.C.; de Oliveira,L.R.; Bonatto,S.L.; Marins,L.F.. |
| The South American sea lion, Otaria flavescens, is widely distributed along the Pacific and Atlantic coasts of South America. However, along the Brazilian coast, there are only two nonbreeding sites for the species (Refúgio de Vida Silvestre da Ilha dos Lobos and Refúgio de Vida Silvestre do Molhe Leste da Barra do Rio Grande), both in Southern Brazil. In this region, the species is continuously under the effect of anthropic activities, mainly those related to environmental contamination with organic and inorganic chemicals and fishery interactions. This paper reports, for the first time, the genetic diversity of O. flavescens found along the Southern Brazilian coast. A 287-bp fragment of the mitochondrial DNA control region (D-loop) was analyzed. Seven... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Otaria flavescens; MtDNA; D-loop; Genetic variability; New haplotypes. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000900002 |
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| Henkes,Luis Ernani; Benavides,Magda Vieira; Oliveira,João Francisco Coelho; Moraes,José Carlos Ferrugem; Weimer,Tania Azevedo. |
| Cytoplasmic inheritance influence on reproductive traits was investigated in the Brangus-Ibagé cattle (3/8 Nelore x 5/8 Aberdeen Angus). Additive genetic effects were responsible for 12% ± 11% of phenotypic variation observed in first calving interval, but their contribution dropped to zero when all calving intervals (CI) were considered. The heritability estimate for age at first calving (AFC, in days) was 0.19 ± 0.09. Mitochondrial lineage (MIT) had negligible effects on phenotypic variances of calving interval (0.0 ± 0.02), calf birth weight (0.0 ± 0.01), and cow weight at calving (0.0 ± 0.01). However, for the age at first calving, MIT accounted for 0.15 ± 0.07 of total variation. Cow weight at calving had a significant linear effect on CI and AFC.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MtDNA; Maternal inheritance; Calving interval. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782004000400030 |
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| Hernández Llamas, Alán Roberto. |
| El venado cola blanca es la especie de mayor importancia en la actividad cinegética en México. En el país se distribuyen 14 de las 38 subespecies reportadas para Norte, Centro y Sur América, a pesar de esto existe poco conocimiento acerca de su diversidad genética. El objetivo de esta investigación fue determinar y describir la variabilidad genética de las subespecies O. v. miquihuanensis, O. v. couesi y O. v. mexicanus en la región Centro-Norte de México por medio del uso de un fragmento de la Región Control (D-Loop) del ADN mitocondrial. Se colectaron 67 muestras de tejido muscular, cartílago auricular u órganos; 4 de O. v. miquihuanensis, 43 O. v. mexicanus, y 20 O. v. couesi de Zacatecas, Aguascalientes, San Luis Potosí, Morelos y Puebla. Se... |
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Palavras-chave: Odocoileus virginianus; ADNmt; Región Control; Diversidad Genética; MtDNA; D-Loop; Genetic Diversity; Ganadería; Maestría. |
| Ano: 2014 |
URL: http://hdl.handle.net/10521/2246 |
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| Kandemir,Irfan; Pinto,Maria A.; Meixner,Marina D.; Sheppard,Walter S.. |
| Restriction fragment length polymorphism of whole mitochondrial DNA or PCR amplified mtDNA regions are known to be useful in discriminating among honey bee lineages and also some individual subspecies. In this study, PCR-amplified fragments of cytochrome oxidase I (CO-I) and cytochrome B (Cyt B) of honey bees sampled from different countries (Cyprus, Turkey, Ethiopia, Syria and Egypt) were digested with Hinf I and Bgl II restriction enzymes, respectively. Eastern Europe and Mediterranean honey bee subspecies were separated by the Cyt B/Bgl II analysis, although Hinf I digestion of the CO-I region yielded much finer resolution within different honey bee lineages. Here we report that CO-I/Hinf-I is a discriminative test for the mitochondrial "O" lineage,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MtDNA; Cytochrome oxidase I; Apis mellifera lamarckii; O lineage. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400027 |
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| Tagliaro,Claudia Helena; Schneider,Horacio; Sampaio,Iracilda; Schneider,Maria Paula Cruz; Vallinoto,Marcelo; Stanhope,Michael. |
| The systematics of the subfamily Callitrichinae (Platyrrhini, Primates), a group of small monkeys from South America and Panama, remains an area of considerable discussion despite many investigations, there being continuing controversy over subgeneric taxonomic classifications based on morphological characters. The purpose of our research was to help elucidate the phylogenetic relationships within the monkey genus Saguinus (Callitrichinae) using a molecular approach to discover whether or not the two different sections containing hairy-faced and bare-faced species are monophyletic, whether Saguinus midas midas and Saguinus bicolor are more closely related than are S. midas midas and Saguinus midas niger, and if Saguinus fuscicollis melanoleucus and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Saguinus; Callitrichinae; ND1; Phylogeny; MtDNA; Conservation. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100009 |
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| Rodrigues-Filho,Luis Fernando da Silva; Rocha,Tainá Carreira da; Rêgo,Péricles Sena do; Schneider,Horacio; Sampaio,Iracilda; Vallinoto,Marcelo. |
| The ongoing decline in abundance and diversity of shark stocks, primarily due to uncontrolled fishery exploitation, is a worldwide problem. An additional problem for the development of conservation and management programmes is the identification of species diversity within a given area, given the morphological similarities among shark species, and the typical disembarkation of processed carcasses which are almost impossible to differentiate. The main aim of the present study was to identify those shark species being exploited off northern Brazil, by using the 12S-16S molecular marker. For this, DNA sequences were obtained from 122 specimens collected on the docks and the fish market in Bragança, in the Brazilian state of Pará. We identified at least 11... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sharks; Molecular identification; Phylogenetic; MtDNA; Conservation. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200031 |
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| Vallinoto,Marcelo; Araripe,Juliana; Rego,Péricles S. do; Tagliaro,Claudia H.; Sampaio,Iracilda; Schneider,Horacio. |
| The Saguinus represent the basal genus of the Callitrichinae subfamily. Traditionally this genus is divided into three groups: Hairy, Mottled and Bare-face, however, molecular data failed to validate these groups as monophyletic units, as well as raised some subspecies to the species status. This is the case of the former subspecies Saguinus midas midas and S. midas niger, which are now considered as different species. In the present study, we sequenced a portion of the D-loop mtDNA region in populations from the East bank of the Xingu and from both banks of the Tocantins river, in order to test the effectiveness of large rivers as barriers to the gene flow in Saguinus. According to our results, the populations from the East and West banks of the Tocantins... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Primates; Saguinus niger; D-loop; MtDNA; Tocantins river. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200005 |
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| Siti-Balkhis,Abu Bakar; Jamsari,Amirul Firdaus Jamaluddin; Hwai,Tan Shau; Yasin,Zulfigar; Siti-Azizah,Mohd Nor. |
| Channa striata, locally known as "haruan", is economically important in fisheries and aquaculture industries in several Asian countries. DNA sequencing, based on a partial segment of the Cytochrome oxidase c subunit 1 (CO1) gene, was used to determine genetic variation in C. striata samples from four different populations on the west coast of Peninsular Malaysia. The highest nucleotide and haplotype diversities were observed in the Linggi population (π = 0.0067, h = 0.835), and the lowest in the Timah Tasoh population (π = 0.0008, h = 0.286). Apart from Kajang-Linggi, which was insignificant, F ST values were significant (p < 0.05) in all pairwise-population comparisons. Consequently, it is inferred that genetic structuring C. striata populations in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Channa striata; Snakehead; MtDNA; CO1; Isolation by distance. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000300024 |
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| Arruda,Cinthya Cristina Bulhões; Beasley,Colin Robert; Vallinoto,Marcelo; Marques-Silva,Nelane do Socorro; Tagliaro,Claudia Helena. |
| Four Brazilian populations of Anomalocardia brasiliana were tested for mutual genetic homogeneity, using data from 123 sequences of the mtDNA cytochrome oxidase c subunit I gene. A total of 36 haplotypes were identified, those shared being H3 (Canela Island, Prainha and Acupe) and both H5 and H9 (Prainha and Acupe). Haplotype diversity values were high, except for the Camurupim population, whereas nucleotide values were low in all the populations, except for that of Acupe. Only the Prainha population showed a deviation from neutrality and the SSD test did not reject the demographic expansion hypothesis. Fst values showed that the Prainha and Acupe populations represent a single stock, whereas in both the Canela Island and Camurupim stocks, population... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MtDNA; COI; Population; Anomalocardia brasiliana; Conservation. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200033 |
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| Registros recuperados: 69 | |
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