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Registros recuperados: 69
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Variabilidad genética y relación filogeográfica de tres subespecies de venado cola blanca (Odocoileus virginianus) en la región Centro-Norte de México. Colegio de Postgraduados
Hernández Llamas, Alán Roberto.
El venado cola blanca es la especie de mayor importancia en la actividad cinegética en México. En el país se distribuyen 14 de las 38 subespecies reportadas para Norte, Centro y Sur América, a pesar de esto existe poco conocimiento acerca de su diversidad genética. El objetivo de esta investigación fue determinar y describir la variabilidad genética de las subespecies O. v. miquihuanensis, O. v. couesi y O. v. mexicanus en la región Centro-Norte de México por medio del uso de un fragmento de la Región Control (D-Loop) del ADN mitocondrial. Se colectaron 67 muestras de tejido muscular, cartílago auricular u órganos; 4 de O. v. miquihuanensis, 43 O. v. mexicanus, y 20 O. v. couesi de Zacatecas, Aguascalientes, San Luis Potosí, Morelos y Puebla. Se...
Palavras-chave: Odocoileus virginianus; ADNmt; Región Control; Diversidad Genética; MtDNA; D-Loop; Genetic Diversity; Ganadería; Maestría.
Ano: 2014 URL: http://hdl.handle.net/10521/2246
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High levels of genetic variability and differentiation in hilsa shad, Tenualosa ilisha (Clupeidae, Clupeiformes) populations revealed by PCR-RFLP analysis of the mitochondrial DNA D-loop region Genet. Mol. Biol.
Mazumder,Sabuj Kanti; Alam,Md. Samsul.
The hilsa shad, Tenualosa ilisha (Clupeidae, Clupeiformes) is an important anadromous clupeid species from the Western division of the Indo-Pacific region. It constitutes the largest single fishable species in Bangladesh. Information on genetic variability and population structure is very important for both management and conservation purposes. Past reports on the population structure of T. ilisha involving morphometric, allozyme and RAPD analyses are contradictory. We examined genetic variability and divergence in two riverine (the Jamuna and the Meghna), two estuarine (Kuakata and Sundarbans) and one marine (Cox's Bazar) populations of T. ilisha by applying PCR-RFLP analysis of the mtDNA D-loop region. The amplified PCR products were restricted with four...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic variability; PCR-RFLP; MtDNA; Tenualosa ilisha.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100029
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Maternal transmission of mitochondrial diseases Genet. Mol. Biol.
Chiaratti,Marcos R.; Macabelli,Carolina H.; Augusto Neto,José Djaci; Grejo,Mateus Priolo; Pandey,Anand Kumar; Perecin,Felipe; Collado,Maite del.
Abstract Given the major role of the mitochondrion in cellular homeostasis, dysfunctions of this organelle may lead to several common diseases in humans. Among these, maternal diseases linked to mitochondrial DNA (mtDNA) mutations are of special interest due to the unclear pattern of mitochondrial inheritance. Multiple copies of mtDNA are present in a cell, each encoding for 37 genes essential for mitochondrial function. In cases of mtDNA mutations, mitochondrial malfunctioning relies on mutation load, as mutant and wild-type molecules may co-exist within the cell. Since the mutation load associated with disease manifestation varies for different mutations and tissues, it is hard to predict the progeny phenotype based on mutation load in the progenitor. In...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Oocyte; Germline; Mitochondrial dynamics; MtDNA; Metabolism.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000200308
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Genetic structure of honeybee populations from southern Brazil and Uruguay Genet. Mol. Biol.
Diniz,Nilza Maria; Soares,Ademilson Espencer Egea; Sheppard,Walter Steve; Del Lama,Marco Antonio.
Apis mellifera scutellata was introduced to Brazil in 1956 and Africanized honeybee populations have now spread from Argentina to the southwestern United States. Temperate climatic restrictions seem to be a natural limit to Africanized honeybee expansion around parallels 35° to 40° SL. We used allozyme loci (Mdh-1 and Hk-1) and mtDNA haplotypes to characterize honeybee populations in southern Brazil and Uruguay and define a possible transition area between Africanized and European bees. Samples of 194 bee colonies were collected from ten localities between 30°-35° SL and 52°-59° WL. The mtDNA restriction patterns of these colonies were obtained through digestion of the mitochondrial genome by Eco RI, or by digestion by Bgl II and Xba I of the cytochrome B...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Africanized honeybees; Transition area; South America; MtDNA; Allozymes; Population genetics.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100008
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Mitochondrial DNA variation in wild populations of Leporinus elongatus from the Paraná River basin Genet. Mol. Biol.
Martins,Cesar; Wasko,Adriane P.; Oliveira,Claudio; Foresti,Fausto.
Leporinus elongatus, a fish species widely distributed throughout the Paraná River basin in South America, is an important fishery resource and a valuable species in aquaculture programs. Despite its great economic importance, several wild populations have been suffering a drastic reduction. The comprehension of its population structure represents an important step for the conservation of these organisms in natural environments, and also for the selection of wild stocks to be used in hatchery programs. In order to understand the genetic-population structure of L. elongatus, D-loop mitochondrial DNA analyses were applied in six wild populations of the species. The results were used to estimate the levels of within and among population genetic variability....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Conservation genetics; D-loop; Genetic variability; Leporinus elongatus; MtDNA.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000100006
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Molecular phylogeny of the genus Saguinus (Platyrrhini, Primates) based on the ND1 mitochondrial gene and implications for conservation Genet. Mol. Biol.
Tagliaro,Claudia Helena; Schneider,Horacio; Sampaio,Iracilda; Schneider,Maria Paula Cruz; Vallinoto,Marcelo; Stanhope,Michael.
The systematics of the subfamily Callitrichinae (Platyrrhini, Primates), a group of small monkeys from South America and Panama, remains an area of considerable discussion despite many investigations, there being continuing controversy over subgeneric taxonomic classifications based on morphological characters. The purpose of our research was to help elucidate the phylogenetic relationships within the monkey genus Saguinus (Callitrichinae) using a molecular approach to discover whether or not the two different sections containing hairy-faced and bare-faced species are monophyletic, whether Saguinus midas midas and Saguinus bicolor are more closely related than are S. midas midas and Saguinus midas niger, and if Saguinus fuscicollis melanoleucus and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saguinus; Callitrichinae; ND1; Phylogeny; MtDNA; Conservation.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100009
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Mitochondrial DNA single nucleotide polymorphism associated with weight estimated breeding values in Nelore cattle (Bos indicus) Genet. Mol. Biol.
Biase,Fernando Henrique; Meirelles,Flávio Vieira; Gunski,Ricardo; Vozzi,Pedro Alejandro; Bezerra,Luiz A.F.; Vila,Reginaldo A.; Rosa,Artur J.M.; Lôbo,Raysildo B.; Martelli,Lúcia.
We sampled 119 Nelore cattle (Bos indicus), 69 harboring B. indicus mtDNA plus 50 carrying Bos taurus mtDNA, to estimate the frequencies of putative mtDNA single nucleotide polymorphisms (SNPs) and investigate their association with Nelore weight and scrotal circumference estimated breeding values (EBVs). The PCR restriction fragment length polymorphism (PCR-RFLP) method was used to detect polymorphisms in the mitochondrial asparagine, cysteine, glycine, leucine and proline transporter RNA (tRNA) genes (tRNAasn, tRNAcys, tRNAgly, tRNAleu and tRNApro). The 50 cattle carrying B. taurus mtDNA were monomorphic for all the tRNA gene SNPs analyzed, suggesting that they are specific to mtDNA from B. indicus cattle. No tRNAcys or tRNAgly polymorphisms were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine; Mitochondria; MtDNA; SNP; Weight.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600005
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Genetic and morphometric differences between yellowtail snapper (Ocyurus chrysurus, Lutjanidae) populations of the tropical West Atlantic Genet. Mol. Biol.
Vasconcellos,Anderson V.; Vianna,Paulo; Paiva,Paulo C.; Schama,Renata; Solé-Cava,Antonio.
Populations of Ocyurus chrysurus were compared genetically and morphometrically along the West Atlantic coast to test the null hypothesis of population homogeneity in the area. Brazilian populations were found to be differentiated in shape (canonical variates analysis; F[48,515] = 10.84, p < 0.0001). Analyses of mitochondrial DNA sequences (663 bp of the control region) did not show any differences between Brazilian populations but could detect differences between Brazilian and Caribbean (Belize) populations. The samples from Pernambuco differed significantly from the other Brazilian populations in allozyme frequencies (11 loci; F ST = 0.167; p < 0.05), but this may have resulted from the small number of samples analysed for that population. Sequence...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MtDNA; Allozymes; Morphometry; Fisheries; Phylogeography.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200026
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Hinf-I digestion of cytochrome oxidase I region is not a diagnostic test for A. m. lamarckii Genet. Mol. Biol.
Kandemir,Irfan; Pinto,Maria A.; Meixner,Marina D.; Sheppard,Walter S..
Restriction fragment length polymorphism of whole mitochondrial DNA or PCR amplified mtDNA regions are known to be useful in discriminating among honey bee lineages and also some individual subspecies. In this study, PCR-amplified fragments of cytochrome oxidase I (CO-I) and cytochrome B (Cyt B) of honey bees sampled from different countries (Cyprus, Turkey, Ethiopia, Syria and Egypt) were digested with Hinf I and Bgl II restriction enzymes, respectively. Eastern Europe and Mediterranean honey bee subspecies were separated by the Cyt B/Bgl II analysis, although Hinf I digestion of the CO-I region yielded much finer resolution within different honey bee lineages. Here we report that CO-I/Hinf-I is a discriminative test for the mitochondrial "O" lineage,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MtDNA; Cytochrome oxidase I; Apis mellifera lamarckii; O lineage.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400027
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Molecular genetic diversity in populations of the stingless bee Plebeia remota: A case study Genet. Mol. Biol.
Francisco,Flávio de Oliveira; Santiago,Leandro Rodrigues; Arias,Maria Cristina.
Genetic diversity is a major component of the biological diversity of an ecosystem. The survival of a population may be seriously threatened if its genetic diversity values are low. In this work, we measured the genetic diversity of the stingless bee Plebeia remota based on molecular data obtained by analyzing 15 microsatellite loci and sequencing two mitochondrial genes. Population structure and genetic diversity differed depending on the molecular marker analyzed: microsatellites showed low population structure and moderate to high genetic diversity, while mitochondrial DNA (mtDNA) showed high population structure and low diversity in three populations. Queen philopatry and male dispersal behavior are discussed as the main reasons for these findings.
Tipo: Info:eu-repo/semantics/article Palavras-chave: MtDNA; Meliponini; Microsatellites; Philopatry; Population genetics.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000100017
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Phylogeography and RAPD-PCR variation in Hoplias malabaricus (Bloch, 1794) (Pisces, Teleostei) in southeastern Brazil Genet. Mol. Biol.
Dergam,Jorge A.; Paiva,Samuel R.; Schaeffer,Carlos E.; Godinho,Alexandre L.; Vieira,Fabio.
In the Rio Doce basin of southeastern Brazil, the freshwater fish Hoplias malabaricus (trahira) is a widespread predatory characin and one of the few resilient native fishes in a highly impacted lake system. In order to test for genetic differentiation in populations within this basin and for biogeographic relationships among populations of this species in other basins, a study was conducted using RAPD-PCR analysis of Rio Doce samples (N = 63) and phylogeographic analyses with mitochondrial DNA (mtDNA) haplotypes, including the Rio Grande and Macacu river basins. In the Rio Doce basin, the patterns of genetic similarity of RAPD-PCR markers (individual fingerprinting and Nei’s genetic distance) suggest the existence of two genetically different groups, one...
Tipo: Info:eu-repo/semantics/article Palavras-chave: RAPD; SSCP; MtDNA; Phylogeography; Hoplias malabaricus; Conservation genetics.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400005
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Human aging and somatic point mutations in mtDNA: a comparative study of generational differences (grandparents and grandchildren) Genet. Mol. Biol.
Marinho,Anderson Nonato do Rosário; Moraes,Milene Raiol de; Santos,Sidney; Santos,Ândrea Ribeiro-dos-.
The accumulation of somatic mutations in mtDNA is correlated with aging. In this work, we sought to identify somatic mutations in the HVS-1 region (D-loop) of mtDNA that might be associated with aging. For this, we compared 31 grandmothers (mean age: 63 ± 2.3 years) and their 62 grandchildren (mean age: 15 ± 4.1 years), the offspring of their daughters. Direct DNA sequencing showed that mutations absent in the grandchildren were detected in a presumably homoplasmic state in three grandmothers and in a heteroplasmic state in an additional 13 grandmothers; no mutations were detected in the remaining 15 grandmothers. However, cloning followed by DNA sequencing in 12 grandmothers confirmed homoplasia in only one of the three mutations previously considered to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aging; MtDNA; Mitochondrial somatic mutations.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100006
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Taxonomic implications of molecular studies on Northern Brazilian Teredinidae (Mollusca: Bivalvia) specimens Genet. Mol. Biol.
Santos,Sonia Maria Lima; Tagliaro,Claudia Helena; Beasley,Colin Robert; Schneider,Horacio; Sampaio,Iracilda; Santos Filho,Carlos; Müller,Ana Cláudia de Paula.
The current taxonomy of the Teredinidae (shipworms) is wholly based on morphology and up to now no molecular studies of the phylogeny of this group have been published. In the present study the relationships between four genera of the subfamilies Teredininae and Bankiinae were established and the efficiency of the 16S rRNA gene in characterizing four Teredinidae species was tested. Phylogenetic trees support the grouping of Bankia fimbriatula with Nausitora fusticula and of Neoteredo reynei with Psiloteredo healdi, but the genetic distances do not justify the classification of these species into two distinct subfamilies. The results show that B. fimbriatula, N. reynei and P. healdi specimens from the coast of the Brazilian state of Pará have five distinct...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Teredinidae; RRNA16S; Phylogeny; MtDNA.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100031
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Genetic signatures of parental contribution in black and white populations in Brazil Genet. Mol. Biol.
Guerreiro-Junior,Vanderlei; Bisso-Machado,Rafael; Marrero,Andrea; Hünemeier,Tábita; Salzano,Francisco M.; Bortolini,Maria Cátira.
Two hundred and three individuals classified as white were tested for 11 single nucleotide polymorphisms plus two insertion/deletions in their Y-chromosomes. A subset of these individuals (n = 172) was also screened for sequences in the first hypervariable segment of their mitochondrial DNA (mtDNA). In addition, complementary studies were done for 11 of the 13 markers indicated above in 54 of 107 black subjects previously investigated in this southern Brazilian population. The prevalence of Y-chromosome haplogroups among whites was similar to that found in the Azores (Portugal) or Spain, but not to that of other European countries. About half of the European or African mtDNA haplogroups of these individuals were related to their places of origin, but not...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Admixture dynamics; MtDNA; Y-chromosome markers.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100001
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Tocantins river as an effective barrier to gene flow in Saguinus niger populations Genet. Mol. Biol.
Vallinoto,Marcelo; Araripe,Juliana; Rego,Péricles S. do; Tagliaro,Claudia H.; Sampaio,Iracilda; Schneider,Horacio.
The Saguinus represent the basal genus of the Callitrichinae subfamily. Traditionally this genus is divided into three groups: Hairy, Mottled and Bare-face, however, molecular data failed to validate these groups as monophyletic units, as well as raised some subspecies to the species status. This is the case of the former subspecies Saguinus midas midas and S. midas niger, which are now considered as different species. In the present study, we sequenced a portion of the D-loop mtDNA region in populations from the East bank of the Xingu and from both banks of the Tocantins river, in order to test the effectiveness of large rivers as barriers to the gene flow in Saguinus. According to our results, the populations from the East and West banks of the Tocantins...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Primates; Saguinus niger; D-loop; MtDNA; Tocantins river.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200005
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Uniparental ancestry markers in Chilean populations Genet. Mol. Biol.
Vieira-Machado,Camilla Dutra; Tostes,Maluah; Alves,Gabrielle; Nazer,Julio; Martinez,Liliana; Wettig,Elisabeth; Pizarro Rivadeneira,Oscar; Diaz Caamaño,Marcela; Larenas Ascui,Jessica; Pavez,Pedro; Dutra,Maria da Graça; Castilla,Eduardo Enrique; Orioli,Ieda Maria.
Abstract The presence of Native Americans, Europeans, and Africans has led to the development of a multi-ethnic, admixed population in Chile. This study aimed to contribute to the characterization of the uniparental genetic structure of three Chilean regions. Newborns from seven hospitals in Independencia, Providencia, Santiago, Curicó, Cauquenes, Valdívia, and Puerto Montt communes, belonging to the Chilean regions of Santiago, Maule, and Los Lagos, were studied. The presence of Native American mitochondrial DNA (mtDNA) haplogroups and two markers present in the non-recombinant region of the Y chromosome, DYS199 and DYS287, indicative of Native American and African ancestry, respectively, was determined. A high Native American matrilineal contribution and...
Tipo: Info:eu-repo/semantics/other Palavras-chave: Uniparental markers; Ancestry; MtDNA; Y-chromosome; ECLAMC.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000400573
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A test of the utility of DNA barcoding in the radiation of the freshwater stingray genus Potamotrygon (Potamotrygonidae, Myliobatiformes) Genet. Mol. Biol.
Toffoli,Daniel; Hrbek,Tomas; Araújo,Maria Lúcia Góes de; Almeida,Maurício Pinto de; Charvet-Almeida,Patricia; Farias,Izeni Pires.
DNA barcoding is a recently proposed global standard in taxonomy based on DNA sequences. The two main goals of DNA barcoding methodology are assignment of specimens to a species and discovery of new species. There are two main underlying assumptions: i) reciprocal monophyly of species, and ii) intraspecific divergence is always less than interspecific divergence. Here we present a phylogenetic analysis of the family Potamotrygonidae based on mitochondrial cytochrome c oxidase I gene, sampling 10 out of the 18 to 20 valid species including two non-described species. Potamotrygonidae systematics is still not fully resolved with several still-to-be-described species while some other species are difficult to delimit due to overlap in morphological characters...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Potamotrygon; Barcoding; Radiation; Phylogenetics; COI; MtDNA.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200028
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Green turtle (Chelonia mydas) genetic diversity at Paranaguá Estuarine Complex feeding grounds in Brazil Genet. Mol. Biol.
Jordão,Juliana Costa; Bondioli,Ana Cristina Vigliar; Guebert,Flavia Maria; Thoisy,Benoit de; Toledo,Lurdes Foresti de Almeida.
Sea turtles are marine reptiles that undertake long migrations through their life, with limited information regarding juvenile stages. Feeding grounds (FGs), where they spend most of their lives, are composed by individuals from different natal origins, known as mixed stock populations. The aim of this study was to assess genetic composition, natal origins and demographic history of juvenile green turtles (Chelonia mydas) at the Paranaguá Estuarine Complex (PEC), Brazil, considered a Natural World Heritage site. Tissue samples of stranded animals were collected (n = 60), and 700 bp mitochondrial DNA sequences were generated and compared to shorter sequences from previously published studies. Global exact tests of differentiation revealed significant...
Tipo: Info:eu-repo/semantics/report Palavras-chave: Chelonia mydas; MtDNA; Feeding grounds; Mixed stock analysis; Connectivity.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000300346
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Significant genetic differentiation among populations of Anomalocardia brasiliana (Gmelin, 1791): a bivalve with planktonic larval dispersion Genet. Mol. Biol.
Arruda,Cinthya Cristina Bulhões; Beasley,Colin Robert; Vallinoto,Marcelo; Marques-Silva,Nelane do Socorro; Tagliaro,Claudia Helena.
Four Brazilian populations of Anomalocardia brasiliana were tested for mutual genetic homogeneity, using data from 123 sequences of the mtDNA cytochrome oxidase c subunit I gene. A total of 36 haplotypes were identified, those shared being H3 (Canela Island, Prainha and Acupe) and both H5 and H9 (Prainha and Acupe). Haplotype diversity values were high, except for the Camurupim population, whereas nucleotide values were low in all the populations, except for that of Acupe. Only the Prainha population showed a deviation from neutrality and the SSD test did not reject the demographic expansion hypothesis. Fst values showed that the Prainha and Acupe populations represent a single stock, whereas in both the Canela Island and Camurupim stocks, population...
Tipo: Info:eu-repo/semantics/article Palavras-chave: MtDNA; COI; Population; Anomalocardia brasiliana; Conservation.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000200033
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Site heteroplasmy in the mitochondrial cytochrome b gene of the sterlet sturgeon Acipenser ruthenus Genet. Mol. Biol.
Dudu,Andreea; Georgescu,Sergiu Emil; Berrebi,Patrick; Costache,Marieta.
Sturgeons are fish species with a complex biology. They are also characterized by complex aspects including polyploidization and easiness of hybridization. As with most of the Ponto-Caspian sturgeons, the populations of Acipenser ruthenus from the Danube have declined drastically during the last decades. This is the first report on mitochondrial point heteroplasmy in the cytochrome b gene of this species. The 1141 bp sequence of the cytb gene in wild sterlet sturgeon individuals from the Lower Danube was determined, and site heteroplasmy evidenced in three of the 30 specimens collected. Two nucleotide sequences were identified in these heteroplasmic individuals. The majority of the heteroplasmic sites are synonymous and do not modify the sequence of amino...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Sterlet sturgeon; MtDNA; Site heteroplasmy.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500024
Registros recuperados: 69
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