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Studies on parasitemia courses and mortality in mice infected with genetically distant Trypanosoma cruzi clonets Biol. Res.
WALLACE,ANNE; ORTÍZ,SILVIA; SÁNCHEZ,GITTITH; VILLAGRA,REBECA; MUGA,MABEL; SOLARI,ALDO.
The biological characterization of bloodstream forms of eleven Trypanosoma cruzi cloned stocks, corresponding to two genetically similar clonets (<A HREF="#19">19</A> and <A HREF="#20">20</A>) and one distant clonet (39), according to multilocus enzyme electrophoresis analysis, showed dissimilar parasitemia in an experimental isogenic mouse model. While clonet 39 stocks gave low parasitemias, clonets 19 or 20 stocks gave high parasitemias, independently of the inocula (10² and 10(4) bloodstream forms) used. High parasitemia did not always associate with greater mortality. Statistical studies on mortality using a low inocula showed significantly higher mortality with clonet 39 stocks when compared to clonets 19 or 20 stocks. Finally,...
Tipo: Journal article Palavras-chave: Trypanosoma cruzi; Clonets; Parasitemia; Mortality; Bloodstream forms; Multilocus enzyme electrophoresis.
Ano: 2001 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200011
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Trypanosoma cruzi Chagas, 1909: genetic variability of isolates from chronic chagasic patients in the Paraná state, Brazil BABT
Kopp,Rogério Luiz; Miyazaki,Márcia; Thomaz-Soccol,Vanete.
The present work had as objective to verify the genetic diversity among strains of Trypanosoma cruzi isolated in chronic chagasic patients in the Paraná state. Fifty patients with compatible clinical symptoms were selected (cardiopathy, megacolon and/or megaesophagus) and that presented positive serological reaction to T. cruzi. Six strains of the protozoan were isolated in hemoculture and identified by electrophoresis in starch thick gel with aid of 14 isoenzymes (6PGD; G6PD; ME¹; ME²; ICD; PGM; GPI; GOT¹; GOT²; NP¹; NP²; DIA; MPI and FH). The statistical analysis were accomplished by the softwares NTSYs and UPGMA. Eighty-four electromorphs were individualized and four isoenzymes (ICD, GPI, 6PGD, e PGM) showed compatibility for heterozygosis. The phenetic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Trypanosoma cruzi; Multilocus enzyme electrophoresis; Chagas disease; Epidemiology; Phenetic analysis.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000300009
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Typing of Streptococcus mutans strains isolated from caries free and susceptible subjects by multilocus enzyme electrophoresis BJM
Tahmourespour,Arezoo; Nabinejad,Abdolreza; Shirian,Hannaneh; Rosa,Edvaldo Antonio Ribeiro; Tahmourespour,Sanaz.
This study was evaluated the clonal diversity of Streptococcus mutans in caries-free and caries-active subjects using MLEE. Strains from caries-free subjects were grouped in a single taxon. Unrooted dendrogram showed that different strains clustered in four different clades, also showed that more than one clonal type can be found in a same individual.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptococcus mutans; Multilocus enzyme electrophoresis; Caries free; Caries susceptible; Typing.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300033
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