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In vitro shoot induction and multiplication from nodal segments of adult Ginkgo biloba plants Horticultura Brasileira
Mantovani,Nilton César; Grando,Magali F; Xavier,Aloisio; Otoni,Wagner C.
The in vitro performance of herbaceous and woody nodal segments from adult plants and the effect of hydrolyzed casein (HC 500 mg L-1), kinetin (KIN; 6-furfurylaminopurine 0.46 and 4.65 µM) and activated charcoal (AC 1.5 g L-1) were evaluated upon new shoots induction and development, and to establish a system of in vitro propagation from adult plants of Ginkgo biloba. Woody nodal segments did not produce axillary shoots and presented 100% of bacterial and fungal contamination in culture. However, nodal segments from herbaceous shoots were successfully disinfected and displayed high in vitro morphogenic capacity. The HC was essential for the axillary shoots induction and further multiplication, stimulating shoot formation in 85% of the cultured nodal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hydrolyzed casein; Activated charcoal; Kinetin; Multiple shoots; Herbaceous and woody branches.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362013000200003
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Genetic stability in rice micropropagation Biocell
Medina,R; Faloci,M; Marassi,M.A.; Mroginski,L.A..
An efficient clonal propagation procedure for six rice varieties cultivated in Argentina was developed by using shoot tip cultures, and the genetic stability of the micropropagated plants was verified by isozyme analysis. One week old seedlings obtained on MS medium were sectioned and subcultured on MS medium (0.75% agar) supplemented with different combination and concentrations of cytokinins (BAP and KIN) and auxins (2,4-D and NAA). After four weeks of culture, multiple shoots were obtained. The best response was observed on MS supplemented with BAP 5 mg l-1. Shoot clumps were multiplied in MS liquid medium containing BAP 5 mg l-1. Profuse rooting was obtained after transfer to MS medium lacking growth regulators and with sucrose 8% (w/v). Complete...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Micropropagation; Multiple shoots; Oryza sativa L.; Isozyme; Genetic stability.
Ano: 2004 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000100002
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