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Registros recuperados: 4
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Successful amplification of mitochondrial DNA from dentin of the bottlenose dolphin Tursiops truncatus BABT
Weber,Laura Isabel; Luca,Michelle Jaccottet de; Barreto,André Silva; Souza,Tiago Tolentino de.
Twenty-four teeth of Tursiops truncatus from the collection of the Marine Mammals Laboratory (FURG-RS) were analysed for their potential as DNA source for population genetic and taxonomic studies. All teeth were submitted to decontamination, grinding and demineralisation processes. Dentin powder was weighed for DNA extraction, which was evaluated by the amplification of two mitochondrial genes through PCR using universal primers. Cytochrome-b gene was amplified successfully. PCR products showed concentrations of 3-12 ng/µl for dentin powder, which was positively correlated to the quantity of dentin powder used for DNA extraction. To rule out contamination a SSCP was carried out including positive controls, and samples of humans involved in the analysis....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dolphins; Tursiops; Museum specimens; Dentin; Mitochondrial DNA; Cytochrome-b.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000100002
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A simple protocol for the extraction and sequence analysis of DNA from study skin of museum collections Genet. Mol. Biol.
Moraes-Barros,Nadia de; Morgante,João Stenghel.
Museum collections have been widely used as sources of biological samples for molecular biology studies and there are several methodologies and techniques to obtain and analyze DNA from tissues archived in museums, but most of these protocols have been developed for a specific tissue or are commercial kits. We present a simple protocol for extracting and amplifying DNA segments from sloth museum specimens. With this simple protocol we analyzed DNA fragments from 64% of 64 skin samples from three-toed sloths (Bradypus variegatus and Bradypus tridactylus) archived in three different museums: 43 samples from the University of São Paulo Museum of Zoology (Museu de Zoologia da Universidade de São Paulo, MUZUSP) São Paulo, São Paulo, Brazil; 18 samples from the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bradypus; DNA; Dried skins; Museum specimens.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600024
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New discoveries from old sources, with reference to the original bird and mammal fauna of the Mascarene Islands, Indian Ocean Naturalis
Hume, J.P.; Prys-Jones, R.P..
The faunal history of the Mascarene Islands (Mauritius, Réunion and Rodrigues) has been extensively documented, with most information being derived from the fossil record, museum skins, the journals and logbooks of early mariners and contemporary illustrations. However, our research on original sources, including Dutch journals held at The Hague, and archival correspondence and fossil collections, notably at The Natural History Museum, London, and the University Museum of Zoology, Cambridge, indicates that many problems remain to be resolved. This situation has been exacerbated by exchange schemes amongst museums, personal rivalries, misidentification, lack of comparative material, and inadequate provenance of specimens; furthermore, problematic and...
Tipo: Article / Letter to the editor Palavras-chave: Mascarene Islands; Extinct birds; Iconographical evidence; Journals; Diaries; Museum specimens; Fossils; Loans and exchanges; 42.83.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/210798
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The use of museum specimens as source of DNA Naturalis
Cibois, A..
Tipo: Article / Letter to the editor Palavras-chave: Aves; Museum specimens; DNA extraction; 42.83.
Ano: 2005 URL: http://www.repository.naturalis.nl/record/210819
Registros recuperados: 4
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