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| Registros recuperados: 14 | |
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| Rechenmacher,Ciliana; Wiebke-Strohm,Beatriz; Oliveira-Busatto,Luisa A. de; Polacco,Joseph C.; Carlini,Célia R.; Bodanese-Zanettini,Maria H.. |
| Abstract Urease catalyzes the hydrolysis of urea to ammonia and carbon dioxide. The ammonia (nitrogen (N) product of urease activity) is incorporated into organic compounds. Thus, urease is involved in N remobilization, as well as in primary N assimilation. Two urease isoforms have been described for soybean: the embryo-specific, encoded by the Eu1 gene, and the ubiquitous urease, encoded by Eu4. A third urease-encoding gene was recently identified, designated Eu5, which encodes the putative protein product SBU-III. The present study aimed to evaluate the contribution of soybean ureases to seed germination and plant development. Analyses were performed using Eu1/Eu4/Eu5-co-suppressed transgenic plants and mutants of the Eu1 and Eu4 urease structural genes,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Plant development; Functional study; Transgenic plants; Mutants; Urease. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000200209 |
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| Almeida,Rosana de; Soares,Ademilson Espencer Egea. |
| The dark coloration of insects eyes is attributed to the accumulation of the brown pigment insectorubin, a mixture of ommochromes, xanthommatin and several ommins, biosynthesized from tryptophan. When any of the events in the synthesis chain is interrupted, formation and accumulation of pigments other than insectorubin occurs, and a new eye color will appear. The aim of the present work is to evaluate the longevity of worker honey bees Apis mellifera, homozygous and heterozygous for the mutant alleles cream (cr), snow-laranja (s la) and brick (bk). Eye pigmentation and average longetivity of bees are very closely related. Mutant bees carrying lighter eye pigmentation are unable to return to the hive; there is, therefore, a close association between the eye... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Eyes; Mutants; Cream; Snow-laranja; Brick. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000200011 |
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| Gaion,Lucas Aparecido; Lorevice,Paulo Guilherme; Monteiro,Carolina Cristina; Gavassi,Marina Alves; D’Amico-Damião,Victor; Gratão,Priscila Lupino; Gasparino,Eduardo Custódio; Carvalho,Rogério Falleiros. |
| ABSTRACT It is well known that phytochromes mediate a wide range of photomorphogenic processes in plants. In addition, many studies have demonstrated the involvement of phytochromes as part of abiotic stress signaling responses. However, little is known about cadmium (Cd) stress regulation by phytochromes. Thus, in this study, we used the phyA (far red-insensitive; fri), phyB1 (temporary redinsensitive; tri) and phyB2 (phyB2) tomato (Solanum lycopersicum L.) mutants to investigate the roles of these three phytochromes on Cd stress responses. The plants were grown over a 21-d period in the presence of Cd. We evaluated plant growth, Cd and chlorophyll content and anatomical changes in the leaves. The results indicated that all genotypes were affected by Cd... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Abiotic stress; Heavy metal; Mutants; Plant anatomy; Solanum lycopersicum. L. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052018000100013 |
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| Duarte,T.R.; Oliveira,S.S.; Macrae,A.; Cedrola,S.M.L.; Mazotto,A.M.; Souza,E.P.; Melo,A.C.N.; Vermelho,A.B.. |
| Keratinases are enzymes of great importance involved in pathogenic processes of some fungi. They also have a widespread ecological role since they are responsible for the degradation and recycling of keratin. On the one hand, studying them furthers our knowledge of pathogenicity mechanisms, which has important implications for human health, and on the other hand, understanding their ecological role in keratin recycling has biotechnological potential. Here, a wild-type keratinolytic Candida parapsilosis strain isolated from a poultry farm was treated with ethyl methanesulfonate in order to generate mutants with increased keratinase activity. Mutants were then cultured on media with keratin extracted from chicken feathers as the sole source of nitrogen and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Candida parapsilosis; Keratinase; Ethyl methanesulfonate; Mutants. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000300006 |
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| Freire,Laurineide Lopes de Carvalho; Costa,Ana Bolena Lima da; Góes,Larissa Brandão; Oliveira,Neiva Tinti de. |
| Five mutants (MaE10, MaE27, MaE24, MaE41 e MaE49) of Metarhizium anisopliae wild strain E9 were analysed for DNA profile through the RAPD technique and for changes in total protein content by spectrophotometry, polyacrylamide gel electrophoresis and densitometry. The pattern of RAPD markers showed genetic polymorphism among the strains: out of twenty primers seven were selected, producing 113 bands. Forty seven bands were present in all strains (41.6% of monomorphic bands) and 66 showed polymorphism (58.4%). The mean coefficient of similarity among all strains was 0.75 (75%). The total protein content varied, staining in the interval of 6.0-8.0 µg/µl. The electrophoresis analysis, through zymogram and protein fraction profiles by densitometry, allowed the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metarhizium anisopliae; Mutants; RAPD; Total protein. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000200004 |
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| Fornazier,Ricardo Francisco; Azevedo,Ricardo Antunes; Ferreira,Renato Rodrigues; Varisi,Vanderlei Aparecido. |
| Lysine is an essential amino acid, synthesized in plants in the aspartic acid pathway. The lysine catabolism is performed by the action of two consecutive enzymes, lysine 2-oxoglutarate reductase (LOR) and saccharopine dehydrogenase (SDH). The steady state of lysine is controlled by both, synthesis and catabolism rates, with the final soluble lysine concentration in cereal seeds a direct result of these processes. In the last 40 years, the enzymes involved in lysine biosynthesis have been purified and characterized from some plant species such as carrot, maize, barley, rice, and coix. Recent reports have revealed that lysine degradation might be related to various physiological processes, for instance growth, development and response to environmental... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amino acids; Lysine; Metabolism; Mutants. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202003000100002 |
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| Registros recuperados: 14 | |
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