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	Provedor de dados BJID (1) BJM (1) Autor Ekrami,Alireza (1) Hajalilou,Abdollah (1) Jorfi,Raheleh (1) Li,Fan (1) Motallebi,Roya (1) Rahmani-Cherati,Tavoos (1) Salleh,Mohamad Amran Mohd (1) Shojaei,Taha Roodbar (1) Tabatabaei,Meisam (1) Wang,Haihe (1) Zhao,Chunyan (1) Mais... Palavra-chave Mycobacterium tuberculosis complex (MTBC) (2) Cadmium-telluride quantum dots (CdTe-QDs) (1) Detection (1) Diagnosis (1) Fluorescence resonance energy transfer (FRET) (1) Gold nanoparticles (AuNPs) (1) Identification (1) Rapid methods (1) Sandwich-form FRET-based biosensor (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2014 (1) 2011 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Development of sandwich-form biosensor to detect Mycobacterium tuberculosis complex in clinical sputum specimens BJID Shojaei,Taha Roodbar; Salleh,Mohamad Amran Mohd; Tabatabaei,Meisam; Ekrami,Alireza; Motallebi,Roya; Rahmani-Cherati,Tavoos; Hajalilou,Abdollah; Jorfi,Raheleh. Mycobacterium tuberculosis, the causing agent of tuberculosis, comes second only after HIV on the list of infectious agents slaughtering many worldwide. Due to the limitations behind the conventional detection methods, it is therefore critical to develop new sensitive sensing systems capable of quick detection of the infectious agent. In the present study, the surface modified cadmium-telluride quantum dots and gold nanoparticles conjunct with two specific oligonucleotides against early secretory antigenic target 6 were used to develop a sandwich-form fluorescence resonance energy transfer-based biosensor to detect M. tuberculosis complex and differentiate M. tuberculosis and M. bovis Bacille Calmette–Guerin simultaneously. The sensitivity and specificity... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium tuberculosis complex (MTBC); Cadmium-telluride quantum dots (CdTe-QDs); Gold nanoparticles (AuNPs); Fluorescence resonance energy transfer (FRET); Sandwich-form FRET-based biosensor. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000600600 Rapid identification of Mycobacterium tuberculosis complex by a novel hybridization signal amplification method based on self-assembly of DNA-streptavidin nanoparticles BJM Wang,Haihe; Zhao,Chunyan; Li,Fan. Rapid detection of Mycobacterium tuberculosis complex (MTBC) is a critical step in controlling tuberculosis (TB). In this study, we used IS6110 as the specific identification target to develop a novel hybridization signal amplification method (HSAM) for the rapid and direct detection of MTBC from clinical sputum specimens. This system consists of magnetic bead-linked capture probes for target isolation, dextranbased nanoparticles for amplifying the reporter molecule (biotinylated-FITC), and detection probes (2B-DNA) for binding the nanoparticles. Both the capture and detection probes were specific to the IS6110 target sequence. Our results determined that as few as 10 copies of the IS6110 sequence or 10 M. tuberculosis bacteria could be detected,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycobacterium tuberculosis complex (MTBC); Detection; Diagnosis; Rapid methods; Identification. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300016 Registros recuperados: 2 Primeira ... 1 ... Última

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