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The epiphytic orchids Ionopsis utricularioides and Psygmorchis pusilla associate with different Ceratobasidium lineages at Valle del Cauca, Colombia Acta Botanica
Valadares,Rafael Borges da Silva; Otero,Joel Tupac; Pereira,Marlon Corrêa; Cardoso,Elke Jurandy Bran Nogueira.
In Orchidaceae, association with symbiotic fungi is required for seed germination and seedling development, thereby being the main energy source during the first steps of germination. Colombia is one of the countries with the greatest biodiversity of orchids, with an estimated 3,200 species, but few studies on orchid mycorrhiza have been conducted. In our study, we isolated and sequenced the internal transcribed spacer rDNA region of fungi from two co-occurring Colombian epiphytic orchids, I. utricularioides and P. pusilla, both belonging to the subtribe Oncidiinae. All sequences were recognized as belonging to the genus Ceratobasidium, known to be a common orchid mycorrhizal fungus in both tropical and temperate orchids. One sequence was 100% similar to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Co-existence; ITS phylogeny; Mycorrhiza; Orchidaceae; Specificity.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000100040
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The effect of different soil properties on arbuscular mycorrhizal colonization of peanuts, sorghum and maize Acta Botanica
Carrenho,Rosilaine; Trufem,Sandra Farto Botelho; Bononi,Vera Lúcia Ramos; Silva,Eraldo Schunk.
Arbuscular mycorrhizal fungi (AMF) are important for plant growth since they increase mineral influx. However, symbiosis efficiency is affected by many environmental factors. This study evaluated the effects of different treatments (+/- phosphorus; +/- liming; +/- organic matter; field, sandy or clayey soil textures) on root colonization (RC) of peanuts, sorghum and maize. The combination of these resulted in seventy-two treatments. The 2×2×2×3×3 factorial experiment was laid out in a randomized design. All data were subjected to variance analysis and the means were compared (Tukey at P<0.05). Three months after seed germination, roots were collected to evaluate the percentage of RC. Results showed that soil texture and liming were the most important...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycorrhiza; Liming; Soil texture; Organic matter; Phosphorus.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062007000300018
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Cyrtopodium paludicolum germination with two Tulasnella isolates Acta Botanica
Carvalho,Otieres Cirino de; Paiva Neto,Vespasiano Borges de; Padilha,Daly Roxana Castro; Veloso,Tomás Gomes Reis; Bocayuva,Melissa Faust; Soares,Deanna Carla Oliveira; Kasuya,Maria Catarina Megumi.
ABSTRACT Symbiosis between orchid seeds and mycorrhizal fungi has been reported to be a determining factor in the success of germination and protocorm development in vitro. The aim of this study was to isolate and identify by molecular analysis the mycorrhizal fungus associated with Cyrtopodium paludicolum, and to evaluate its efficiency in facilitating seed germination and development. Germination experiments were carried out using a fungus isolated from C. paludicolum (CH01) and Epidendrum secundum (M65), which has been successfully used a number of times in symbiotic germination. The experiments were conducted in a completely randomized design with treatments of CH01, M65 as well as under asymbiotic conditions. The mycobiont CH01 was successfully...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cerrado; ITS-DNAr; Mycorrhiza; Orchidaceae; Symbiosis.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000100107
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Diversity of arbuscular mycorrhizal fungi in a gypsum mining impacted semiarid area Acta Botanica
Mergulhão,Adália Cavalcanti do Espírito Santo; Burity,Hélio Almeida; Goto,Bruno Tomio; Maia,Leonor Costa.
The aim of this work was to evaluate the impact of gypsum mining on the occurrence and diversity of arbuscular mycorrhizal fungi (AMF). Soil samples were collected in the rainy and dry periods in four areas of a gypsum mine: native preserved "caatinga" (NC); mine vicinity (MS); reject area (RA); interface between the reject area and an area of "caatinga" degraded by mining (IN). Forty two plant species were identified in these areas; some were not found in both collection periods. Thirty six taxa of AMF were identified: 31 in the rainy and 31 in the dry period, with different species composition. Mining activity reduced diversity of plants and of AMF in the three mining areas when compared to NC. Plant similarity indexes were low between NC and the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Degraded area; Gypsum; Mycorrhiza; Taxonomy.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062010000400020
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Isolation and molecular characterization of Rhizoctonia-like fungi associated with orchid roots in the Quadrilátero Ferrífero and Zona da Mata regions of the state of Minas Gerais, Brazil Acta Botanica
Nogueira,Ricardo Eustáquio; Berg,Cassio van den; Pereira,Olinto Liparini; Kasuya,Maria Catarina Megumi.
Mycorrhizal associations can be considered required for orchids, which depend on the fungi for germination and establishment in natural conditions. Knowledge of the mycorrhizal fungi is important for programs aimed at the reintroduction, conservation and management of orchid species. The objective of this study was the molecular characterization of Rhizoctonia-like fungi from orchids in the Quadrilátero Ferrífero ("Iron Quadrangle") and Zona da Mata ("Forest Zone") regions of the state of Minas Gerais, Brazil. The affinities of these fungi were studied by comparing the rRNA internal transcribed spacer region with that of other isolates and sequences in GenBank. Three isolates had an affinity for Epulorhiza repens, and one was the holotypeof E. epiphytica.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Epulorhiza repens; E. epiphytica; ITS; Tulasnella calospora; Orchidaceae; Mycorrhiza.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200017
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Arbuscular mycorrhizal fungi in a semi-arid, limestone mining-impacted area of Brazil Acta Botanica
Teixeira-Rios,Thaís; Souza,Renata Gomes de; Maia,Leonor Costa; Oehl,Fritz; Lima,Cláudia Elizabete Pereira.
The main goal of this study was to determine the diversity and activity of arbuscular mycorrhizal fungi (AMF) in an area degraded by limestone mining within the semi-arid region of Brazil known as the caatinga (shrublands). Near a limestone quarry, we selected two areas of caatinga (preserved and degraded) for study. The number of glomerospores did not differ significantly between the two areas. There was a trend toward the most probable number of infective propagules being higher in the degraded area. Twenty AMF taxa were identified in the two sampled areas, species richness, diversity and evenness being higher in the preserved area. Two species of Racocetra represent new records for the semi-arid region of Brazil. Glomerospore production and AMF species...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Caatinga; Glomerospores; Infective propagules; Mining activities; Mycorrhiza.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062013000400006
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Avaliação nutricional de mudas de Acacia mangium, Sesbania virgata e Eucalyptus camaldulensis inoculadas com fungos micorrízicos, em casa-de-vegetação e em cava de extração de argila - DOI: 10.4025/actasciagron.v31i4.1364 Agronomy
Schiavo, Jolimar Antonio; UEMS; Martins, Marco Antonio; UENF; Rodrigues, Luciana Aparecida; Instituto Superior de Tecnologia.
O presente trabalho objetivou avaliar o efeito de fungos micorrízicos arbusculares (FMAs) na nutrição de mudas de acácia (Acacia mangium Willd.), sesbânia (Sesbania virgata (Cav.) Pers.) e eucalipto (Eucalyptus camaldulensis Dehnh.) em casa-de-vegetação, bem como a influência dos FMAs, do monocultivo e/ou consórcio sobre os teores dos nutrientes nas folhas das plantas em cava degradada pela extração de argila. Em casa-de-vegetação, mudas de acácia inoculadas com FMAs tiveram incrementos nos conteúdos de N, P e Zn de 22, 71 e 67%, respectivamente; as de sesbânia, de 39, 49, 56, 24, 105 e 54%, respectivamente para N, P, Ca, Mg, Mn e Zn. Na cava de extração de argila, plantas de acácia consorciadas com sesbânia apresentaram menor teor de Ca nas folhas. Ainda,...
Tipo: Campo Palavras-chave: 5.02.01.07-7 Solos Florestais monocultivo; Consórcio; Estado nutricional; Micorriza; Rizóbio Microbiologia e Bioquímica do Solo single planting; Intercropping; Nutritional status; Mycorrhiza; Rhizobium.
Ano: 2009 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/1364
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Symbiotic compatibility between arbuscular mycorrhizal fungi (autoctone or exotic) and three native species of the Caatinga in different phosphorus levels Biological Sciences
Oliveira, João Ricardo Gonçalves de; Resende, Geraldo Milanez de; Melo, Natoniel Franklin de; Yano-Melo, Adriana Mayumi.
The aim of this study was to evaluate three plant species native to Caatinga (Aspidosperma pyrifolium, Ziziphus joazeiro and Pseudobombax simplicifolium) in response to inoculation with two arbuscular mycorrhizal fungi (autoctone: Claroideoglomus etunicatum or exotic: Acaulospora longula) and phosphate fertilization. The experiment was conducted in a greenhouse with a completely randomized design involving a factorial arrangement of three inoculation treatments (control, C. etunicatum or A. longula) and 4 levels of single superphosphate (0 - no added P2O5, 12, 24 and 48 mg dm-3) in 10 repetitions. Under the conditions of this study, the inoculum of C. etunicatum, autoctone to the semi-arid region, showed great compatibility and efficiency in promoting...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 2.12.02.00-1 Semi-arid; Mycorrhiza; Endemic species. Microbiologia aplicada.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/33486
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Seedlings of cashew trees of the brazilian cerrado inoculated with arbuscular mycorrhizal fungi and phosphate-solubilizing microorganisms Agrociencia
Cabral,J. Silva Rodrigues; Assis,Kerlley Cristina-de; Silva,Fabiano Guimarães; Souchie,Edson Luiz; Carneiro,M. Aurélio Carbone.
Soil mictobiota catties out important functions in ecosystems, since it influences growth, mineral nutrition and plant health. Phosphorus (P) is the most limiting nutrient in tropical soils and P-solubilizing microorganisms (PSMs) and the arbuscular mycorrhizal fungi (AMF) are the most important groups of the soil microbial community. The aim of this study was to evaluate the effect of inoculating PSMs and AMF on the development of cashew trees (Anacardium othonianum Rizzini) from the Brazilian Cerrado growing on different substrates. An experiment was carried out in a greenhouse and the experimental design was completely randomized with a factorial 4 × 2 arrangement of treatments: PSMs, AMF, PSMs + AMF and the control. In addition, two substrates were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anacardium othonianum; Promotion of plant growth; Mycorrhiza.
Ano: 2012 URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952012000800006
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Is arbuscular mycorrhizal fungal species community affected by cotton growth management systems in the Brazilian Cerrado? Anais da ABC (AABC)
NUNES,HELIAB B.; GOTO,BRUNO T.; COIMBRA,JOÃO LUIZ; OLIVEIRA,JAMILE S.; TAVARES,DÉRICA G.; ROCHA,MARCELO S.; SILVA,FABIANE L.; SOARES,ANA CRISTINA F..
Abstract: Conventional cotton production in western Bahia, Brazil, involves intensive use of agricultural inputs and mechanization, which may affect arbuscular mycorrhizal fungi (AMF). This work aimed at studying the impact of conventional and organic cotton production in the AMF of western Bahia. Soil samples were obtained from conventional white cotton and colored cotton organic production systems as well as from native Cerrado areas, close to the white cotton fields, and from the subcaducifolia vegetation, close to the organic colored cotton farms. The most frequent species in the conventional farming areas belonged to the genera Acaulospora (10 spp.); Glomus (8 spp.); Dentiscutata (3 spp.); Ambispora, Pacispora and Scutellospora (2 spp. each), as well...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycorrhiza; BRS Safira cotton variety; BRS 336 variety; Glomeromycota.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000700858
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Successive cultivation of maize and agricultural practices on root colonization, number of spores and species of arbuscular mycorrhizal fungi BJM
Carrenho,Rosilaine; Silva,Eraldo Schunk; Trufem,Sandra Farto Botelho; Bononi,Vera Lucia Ramos.
A large number of propagules and a broad spectrum of species are two important components of ecosystem (including agroecosystem) sustainability. Previous studies carried out in temperate areas showed that repeated monoculture leads to a decrease in the species abundance of arbuscular mycorrhizal fungi (AMF). This study evaluated the influence of maize monoculture and its agricultural practices on AMF during three consecutive cropping years in a Brazilian field. At the end of each cycle, soil and root samples were evaluated for species composition, spore populations and root colonization by AMF. The AMF community present during this period was scored according to the Spearman rank correlation and Principal Components Analysis. The mean percent root...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Monoculture; Maize; Mycorrhiza; Diversity of species.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400002
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Enrichment of arbuscular mycorrhizal fungi in a contaminated soil after rehabilitation BJM
Lopes Leal,Patrícia; Varón-López,Maryeimy; Gonçalves de Oliveira Prado,Isabelle; Valentim dos Santos,Jessé; Fonsêca Sousa Soares,Cláudio Roberto; Siqueira,José Oswaldo; de Souza Moreira,Fatima Maria.
Abstract Spore counts, species composition and richness of arbuscular mycorrhizal fungi, and soil glomalin contents were evaluated in a soil contaminated with Zn, Cu, Cd and Pb after rehabilitation by partial replacement of the contaminated soil with non-contaminated soil, and by Eucalyptus camaldulensis planting with and without Brachiaria decumbens sowing. These rehabilitation procedures were compared with soils from contaminated non-rehabilitated area and non-contaminated adjacent soils. Arbuscular mycorrhizal fungi communities attributes were assessed by direct field sampling, trap culture technique, and by glomalin contents estimate. Arbuscular mycorrhizal fungi was markedly favored by rehabilitation, and a total of 15 arbuscular mycorrhizal fungi...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glomeromycota; Heavy metals; Soil contamination; Mycorrhiza; Revegetation.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000400853
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Production and infectivity of inoculum of arbuscular mycorrhizal fungi multiplied in a substrate supplemented with Tris-HCl buffer BJM
Silva,Fábio Sérgio Barbosa da; Yano-Melo,Adriana Mayumi; Maia,Leonor Costa.
The effect of adding Tris-HCl buffer on production and infectivity of AMF inoculum was investigated. Sporulation of Glomus etunicatum, Acaulospora longula and Gigaspora albida was improved in solution with buffer. The infectivity of G. etunicatum increased after storage, what suggests that the inoculum of this isolate is benefited by storage.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycorrhiza; Organic buffer; Sporulation; Storage.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400030
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The interactive effect of phosphorus and nitrogen on "in vitro" spore germination of Glomus etunicatum Becker &amp; Gerdemann, root growth and mycorrhizal colonization BJM
Bressan,Wellington.
The effects of P and N amendment and its interactions on spore germination, root growth and colonized root length by Glomus etunicatum Becker &amp; Gerdemann (INVAM S329) was studied "in vitro" in RiT - DNA transformed roots of Anthylis vulneraria sub sp. Sampaiana (Kidney vetch). Three N media concentrations (5, 10 and 50 mg/l) at P constant level (2 mg/l) and three P media concentrations (2, 10 and 20 mg/l) at N constant level (5 mg/l) were utilized as a treatment. Bécard &amp; Fortin medium was used as a basal medium for root growth and colonized root length, and water/agar (0.8%) media was the control for spore germination. Spore germination of G. etunicatum at low P level was reduced by N addition in relation to the control media, and at low N...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycorrhiza; Glomus etunicatum; Spore germination; Root growth; Root colonization.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400004
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An outlook on ion signaling and ionome of mycorrhizal symbiosis Braz. J. Plant Physiol.
Ramos,Alessandro C.; Façanha,Arnoldo R.; Palma,Livia M.; Okorokov,Lev A.; Cruz,Zilma M.A.; Silva,Ary G.; Siqueira,Arthur F.; Bertolazi,Amanda A.; Canton,Gabriela C.; Melo,Juliana; Santos,Wolmen O.; Schimitberger,Vanusa M. B.; Okorokova-Façanha,Anna L..
The 450-million-year-old interaction between the majority of land plants and mycorrhizal fungi is one of the most ancient, abundant, and ecologically important symbiosis on earth. The early events in the evolution of mycorrhizal symbioses seem to have involved reciprocal genetic changes in ancestral plants and free-living fungi. new data on the mechanism of action of specific signaling molecules and how it influence and is influenced by the membrane ions fluxes and cytoplasm ion oscillations which integrate the symbiotic ionome are improving our understanding of the molecular bases of the mycorrhization process. This mini-review will highlight topics regarding what is known about the ionome and ionic communication in the arbuscular mycorrhizal symbiosis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ion dynamics; Íon signaling; Mycorrhiza; Ionomics; Plant synapsis.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202011000100010
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Effect of Arbucula Myrrhizal Fungi on an Ecological Crop of Chili Peppers (Capsicum annuum L.) Chilean J. Agric. Res.
Castillo R,Claudia ; Sotomayor S,Leonardo ; Ortiz O,César; Leonelli C,Gina; Borie B,Fernando; Rubio H,Rosa.
Mapuche farmers in southern Chile have been cultivating local ecotypes of chili pepper (Capsicum annuum L.), called locally “Cacho de cabra” for many decades. It is used to make “merkén”, a condiment that is consumed locally and exported. This vegetable requires a nursery stage and can obtain nutritional benefits from symbiotic associations such as mycorrhizal fungi, achieving a better adaptation to transplanting. Arbuscular mycorrhizal fungi (AMF) are obligate biotrophes appearing in abundance in agroecosystems with conservation management. The aim of this study was to compare effectiveness of two AMF, a commercial mycorrhizal inoculant (IC, Glomus intraradices) and another native (IN, Glomus claroideum) with a control without inoculation (-I) on the...
Tipo: Journal article Palavras-chave: Mycorrhiza; Inoculant; Biofertilizers; Fruit quality; Vegetables.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000100010
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Long-term phosphate fertilization, mycorrhizal inoculation and historical land use influence on soybean growth and P uptake Ciência e Agrotecnologia
Motta,Paulo Emílio Ferreira da; Siqueira,José Oswaldo; Ribeiro,Bruno Teixeira; Norton,Lloyd Darrell; Silva,Sérgio Henrique Godinho; Curi,Nilton.
ABSTRACT Phosphorus is a key-nutrient in the fertility management of highly weathered tropical soils. So, this work was carried out with the objective of evaluating the influence of the interaction between P doses, mycorrhizal inoculation and historical land use on soybean growth and P uptake in five Latosols (Oxisols) with contrasting chemical, physical and mineralogical properties under a continuous long-term phosphate fertilization (more than 15 years). The plants were cultivated in 4.5L-plastic pots containing 4 kg of soil in a completely randomized design, four replications and 2x2x2 factorial scheme with two P doses; and with or without mycorrhizal inoculation; and soils cultivated for long periods and non-cultivated (under native vegetation). There...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mycorrhiza; Latosols (Oxisols); Available P; Long-term cultivation.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542016000400418
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Soil fungal and bacterial biomass determined by epifluorescence microscopy and mycorrhizal spore density in different sugarcane managements Ciência Rural
Aleixo,Adriana Pereira; Kaschuk,Glaciela; Alberton,Odair.
Crop productivity and sustainability have often been related to soil organic matter and soil microbial biomass, especially because of their role in soil nutrient cycling. This study aimed at measuring fungal and bacterial biomass by epifluorescence microscopy and arbuscular mycorrhizal fungal (AMF) spore density in sugarcane (Saccharum officinarum L.) fields under different managements. We collected soil samples of sugarcane fields managed with or without burning, with or without mechanized harvest, with or without application of vinasse and from nearby riparian native forest. The soil samples were collected at 10cm depth and storage at 4°C until analysis. Fungal biomass varied from 25 to 37µg C g-1 dry soil and bacterial from 178 to 263µg C g-1 dry soil....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Saccharum officinarum; Soil microbial biomass; Mycorrhiza.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000400002
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Arbuscular mycorrhizal fungi and colonization stimulant in cotton and maize Ciência Rural
Salgado,Fabrício Henrique Moreira; Moreira,Fátima Maria de Sousa; Siqueira,José Oswaldo; Barbosa,Ricardo Henrique; Paulino,Helder Barbosa; Carneiro,Marco Aurélio Carbone.
ABSTRACT: The objective of this study was to evaluate the effects of inoculation with different arbuscular mycorrhizal fungi native to Cerrado, associated with the application of colonization stimulant (7-hydroxy, 4’-methoxy-isoflavone) at the initial growth of cotton and maize plants in a greenhouse. The experiment was carried out in a completely randomized design and 7x2 factorial scheme, with five species of arbuscular mycorrhizal fungi and combined inoculation (uniting all species in equal proportion) and native fungi, in the presence and absence of colonization stimulant in maize and cotton crops. Shoots dry matter (SDM), roots dry matter (RDM), mycorrhizal colonization and accumulation of calcium, zinc and phosphorus in the SDM were evaluated at...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Zea may; Gossypium hirsutum L; Formononetin; Mycorrhiza.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000600202
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Aspectos ecofisiológicos de la raíz de Pinus pseudostrobus y P. patula y especies ectomicorrízicas comestibles de Hebeloma spp. y Laccaria spp. Colegio de Postgraduados
Carrasco Hernández, Violeta.
Un alto porcentaje de mortandad se presenta en pinos que son transplantados de vivero a campo, debido a que no poseen en sus raíces hongos ectomicorrízicos los cuales forman simbiosis obligada con los pinos de forma natural en los bosques. Estos hongos facilitan la absorción de nutrimentos y agua, además de estimular su crecimiento. Debido a la importancia de los hongos ectomicorrízicos, el presente trabajo evaluó el efecto en crecimiento, peso seco, porcentaje de colonización y contenido nutrimental de la inoculación de seis especies ectomicorrízicas comestibles de los géneros Laccaria y Hebeloma en Pinus patula y P. pseudostrobus, en condiciones de invernadero. Las especies inoculadas fueron previamente caracterizadas. Después de 397 días de la siembra...
Palavras-chave: Hongo silvestre; Inóculo; Micorriza; Pinos; Fungi wild; Inoculum; Mycorrhiza; Pines; Maestría; Edafología.
Ano: 2010 URL: http://hdl.handle.net/10521/101
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