|
|
| Amaral,Marta G; Campos,Vinicius F; Seixas,Fabiana K; Cavalcanti,Paulo V; Selau,Lisiane P. R; Deschamps,Joao C; Collares,Tiago. |
| Testis-mediated gene transfer (TMGT) has been used as in vivo gene transfer technology to introduce foreign DNA directly into testes, allowing mass gene transfer to offspring via mating. In this study, we used plasmid DNA (pEGFP-N1) mixed with dimethylsulfoxide (DMSO), N,N-dimethylacetamide (DMA) or liposome (Lipofectin) in an attempt to improve TMGT. Males receiving consecutive DNA complex injections were mated to normal females to obtain F0 progeny. In vivo evaluation of EGFP expression, RT-PCR and PCR were used to detect the expression and the presence of exogenous DNA in the progeny. We also evaluated possible testicular damage by histological procedures. PC R and RT-PCR analyses revealed that liposome and DMSO increased the rate of TMGT. Histological... |
| Tipo: Journal article |
Palavras-chave: TMGT DMA (N; N-dimethylacetamide) Liposome mice transgenesis histological damage. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000300003 |
| |
