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MORPHOMETRY AND QUANTIFICATION OF THE MYENTERIC NEURONS OF THE DUODENUM OF ADULT RATS FED WITH HYPOPROTEIC CHOW International Journal of Morphology
Marçal Natali,Maria Raquel; Hubner de Miranda-Neto,Marcílio; Orsi,Antônio Marcos.
Morphometric and quantitative analyses were carried out on the myenteric neurons of the duodenum, to study possible alterations resulting from a supply of low-protein level chow to adult Wistar rats. The animals were divided in two groups: nourished (N) fed with ration containing 22% protein for 210 days and the undernourished (D) fed with ration containing 8% protein from the 90th day of age on and during the next 120 days. Whole-mounts were stainedby the method of Giemsa and the histochemical reaction of NADH-diaphorase enzyme, to estimate the neuronal population, which revealed a greater neuronal density in undernourished rats, regardless of the technique employed. The morphometric studies of the cell body profiles of 500 neurons of each group indicated...
Tipo: Journal article Palavras-chave: Proteic deficiency; Duodenum; GIEMSA; Myenteric neurons; NADH-diaphorase; Rats.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000400003
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Morphoquantitative Study of Rattus norvegicus Submucosal Plexus by Different Neuronal Evidentiation Histochemical Techniques International Journal of Morphology
Góis,Marcelo Biondaro; Hermes-Uliana,Catchia; Paltanin,Alessandra; Pontes,Willian; Araújo,Eduardo José De Almeida; Miranda Neto,Marcilio Hubner; Sant'Ana,Débora Mello Gonçales.
Enteric nervous plexuses have been the object of several studies, specially the myenteric plexus whose studies describe its organization, functions and alterations. On the other hand, the submucosal plexus has been less studied and still needs descriptive studies. To analyze morphologically and quantitatively submucosal neurons of the jejunum of 90-day-old healthy rats using different techniques for neuronal staining as a way to provide normality data to compare with future experimental studies. Whole mount preparations of the jejunum were submitted to Giemsa, NADH-diaphorase and NADPH-diaphorase techniques to stain the total neuronal population, more metabolically active subpopulation and subpopulation of nitrergic neurons, respectively. Neurons of the...
Tipo: Journal article Palavras-chave: Enteric nervous system; Meissner's plexus; Giemsa; NADH-diaphorase; NADPH-diaphorase.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000400050
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