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	Provedor de dados BJM (1) Autor Campos,Karen (1) David,Cíntia de (1) Englert,Gelsa Edith (1) Mergener,Michelle (1) Oliveira,Anna Paula de (1) Roehe,Paulo Michel (1) Simonetti,Amauri Braga (1) Mais... Palavra-chave Cell culture (1) Contamination (1) Fetal bovine serum (FBS) (1) Nanobacteria (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2007 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Nanobacteria-like particles: a threat to cell cultures BJM Simonetti,Amauri Braga; Englert,Gelsa Edith; Campos,Karen; Mergener,Michelle; David,Cíntia de; Oliveira,Anna Paula de; Roehe,Paulo Michel. The main goal of this study is to alert researchers who work with cell cultures for the risk of contamination by structures called nanobacteria (NB). NB are tiny structures with size varying from 80 to 500 nm, commonly occurring in clusters and producing a biofilm which contains carbonate or hydroxyl apatite. The most likely source of cell culture contamination by such organisms is serum used as supplement in culture media. The presence of NB leads to a progressive culture deterioration with accumulation of granules (probably phagocytized NB) in cytoplasmic vacuoles, an increasing number of dead cells in the supernatant and degeneration of cells that remained attached to the bottom of the vessel. NB can also be found in culture supernatants where they are... Tipo: Info:eu-repo/semantics/article Palavras-chave: Nanobacteria; Cell culture; Fetal bovine serum (FBS); Contamination. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100032 Registros recuperados: 1 Primeira ... 1 ... Última

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