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Molecular detection and characterization of cpb2 gene in Clostridium perfringens isolates from healthy and diseased chickens J. Venom. Anim. Toxins incl. Trop. Dis.
Tolooe,A; Shojadoost,B; Peighambari,SM.
Clostridium perfringens is an important pathogen in both human and veterinary medicine. Necrotic enteritis (NE) is the most clinically dramatic bacterial enteric disease of poultry induced by C. perfringens. The pathogenicity of this bacterium is associated with the production of extracellular toxins produced by some of its strains, such as beta2 toxin. The exact role of beta2 toxin in NE pathogenesis is still controversial. In the present study, C. perfringens isolates from healthy and diseased poultry flocks from different parts of Iran were analyzed by PCR assay to determine the presence of all variants of the beta2 toxin gene (cpb2). The products of two positive cpb2 PCR reactions were sequenced, compared to each other and to the cpb2 sequences...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium perfringens; Necrotic enteritis; Chicken; Beta2 toxin; Cpb2; Iran.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000100008
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Antimicrobial susceptibility of Clostridium perfringens strains isolated from broiler chickens BJM
Silva,R. O. S.; Salvarani,F.M.; Assis,R.A.; Martins,N.R.S.; Pires,P.S.; Lobato,F.C.F..
Clostridium perfringens is a normal inhabitant of the intestinal tract of chickens as well as a potential pathogen that causes necrotic enteritis and colangio hepatitis. The minimum inhibitory concentration (MIC) of seven different compounds used for therapy, growth promotion or prevention of coccidiosis was determined by agar dilution method for 55 C. perfringens strains isolated from the intestines of broiler chickens. All strains showed high susceptibility to penicillin, avilamycin, monensin and narasin. Only 7.3% of the strains showed an intermediated sensitivity to lincomycin, and 49 (89.1%) were considered susceptible. For tetracycline and bacitracin, 41.8% and 47.3% of strains, respectively, were considered resistant.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Necrotic enteritis; Growth promoters; Anticoccidials; Antibiotics.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200010
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Adhesion and invasion of Clostridium perfringens type A into epithelial cells BJM
Llanco,Luis A.; Nakano,Viviane; Moraes,Claudia T.P. de; Piazza,Roxane M.F.; Avila-Campos,Mario J..
ABSTRACT Clostridium perfringens is the causative agent for necrotic enteritis. It secretes the major virulence factors, and α- and NetB-toxins that are responsible for intestinal lesions. The TpeL toxin affects cell morphology by producing myonecrosis, but its role in the pathogenesis of necrotic enteritis is unclear. In this study, the presence of netB and tpeL genes in C. perfringens type A strains isolated from chickens with necrotic enteritis, their cytotoxic effects and role in adhesion and invasion of epithelial cells were evaluated. Six (27.3%) of the 22 C. perfringens type A strains were harboring the tpeL gene and produced morphological alterations in Vero cells after 6 h of incubation. Strains tpeL (-) induced strong cell rounding after 6 h of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Adhesion; Invasion; Clostridium perfringens type A; Necrotic enteritis; TpeL gene.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400764
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Genotyping and antimicrobial susceptibility of Clostridium perfringens isolated from Tinamidae, Cracidae and Ramphastidae species in Brazil Ciência Rural
Silva,Rodrigo Otávio Silveira; Ferreira Junior,Francisco Carlos; Marques,Marcus Vinícius Romero; Oliveira Junior,Carlos Augusto; Martins,Nelson Rodrigo da Silva; Lobato,Francisco Carlos Faria.
The aim of this study was to isolate, genotype and evaluate the antimicrobial susceptibility of Clostridium perfringens found in species Tinamidae, Cracidae and Ramphastidae in Brazil. C. perfringens was isolated in 13 (5%) out of 260 swab samples and five (8.3%) out of 60 stool samples. All strains were classified as C. perfringens type A, and nine (50%) were positive for the beta-2 toxin-encoding gene. No strains were positive for the necrotic enteritis toxin B-like (NetB)-encoding gene. All isolates were susceptible to penicillin, metronidazole and vancomycin, whereas four (22.2%), five (27.8%) and 13 (72.2%) strains were considered resistant to erythromycin, oxytetracycline and lincomycin, respectively.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Necrotic enteritis; Avian; Toucan.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000300016
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