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| Registros recuperados: 43 | |
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| Daprato,B; Suraniti,A; Loiza,Y; López,C; Sommerfelt,I. E.. |
| Neospora caninum, protozoario intracelular, es causa de signos nerviosos, gastrointestinales, respiratorios y desórdenes musculares en perros. A los fines de estimar la seroprevalencia de anticuerpos anti N. caninum, se analizaron los sueros de 238 caninos que ingresaron al Hospital Escuela de la Facultad de Ciencias Veterinarias de la Universidad de Buenos Aires, en el período 2011 - 2012. Para ello se utilizó la técnica de inmunofluorescencia indirecta (IFI) ( ≥ 1:50), resultando el 27,3% (95% IC= 21,9%- 33,7%) positivas a N. caninum. La mayor prevalencia se observó en caninos del grupo de 2 a 10 años. Se realizó además una encuesta epidemiológica para analizar posibles factores de riesgo. No se encontraron diferencias significativas entre el... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neospora caninum; Inmunofluorescencia indirecta; Caninos; Factores de riesgo. |
| Ano: 2013 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1668-34982013000200004 |
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| Zhang, Houshuang; Compaore, Muller K.A.; Lee, Eung-goo; Liao, Min; Zhang, Guohong; Sugimoto, Chihiro; Fujisaki, Kozo; Nishikawa, Yoshifumi; Xuan, Xuenan. |
| The cross-reactive antigens of Neospora caninum and Toxoplasma gondii are important in the exploration to determine the common mechanisms of parasite-host interaction. In this study, a gene encoding N. caninum apical membrane antigen 1 (NcAMA1) was identified by immunoscreening of a N. caninum tachyzoite cDNA expression library with antisera. from mice immunized with recombinant T gondii apical membrane antigen 1 (TgAMA 1). NcAMA1 was encoded by an open reading frame of 1695 bp, which encoded a protein of 564 amino acids. The single-copy NcAMA1 gene was interrupted by seven introns. NcAMA1 showed 73.6% amino acid identity to TgAMA1. Mouse polyclonal antibodies raised against the recombinant NcAMA1 (rNcAMA1) recognized a 69-kDa native parasite protein by... |
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Palavras-chave: Neospora caninum; Toxoplasma gondii; Apical membrane antigen 1; Cross-reactive; Invasion. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1034 |
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| Leesombun, Arpron; Boonmasawai, Sookruetai; Nishikawa, Yoshifumi. |
| Neosporosis has a worldwide distribution and causes economic losses in farming, particularly by increasing the risk of abortion in cattle. This study investigated the effects of Thai piperaceae (Piper betle, P. nigrum, and P. sarmentosum) extracts on Neospora caninum infections in vitro and in vivo. In an in vitro parasite growth assay based on the green fluorescent protein (GFP) signal, P. betle was the most effective extract at inhibiting parasite growth in human foreskin fibroblast cells (IC50 of GFP-expressing N. caninum parasites, 22.1 mu g/ml). The P. betle extract, at 25 mu g per ml, inhibited parasite invasion into host cells. Furthermore, in two independent experiments, treating N. caninum-infected mice with the P. betle extract for 7 days... |
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Palavras-chave: Neospora caninum; Neosporosis; Piper betle; Piper nigrum; Piper sarmentosum; Ethanol extract. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4578 |
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| Zhou, Mo; Cao, Shinuo; Sevinc, Ferda; Sevinc, Mutlu; Ceylan, Onur; Liu, Mingming; Wang, Guanbo; Moumouni, Paul Franck Adjou; Jirapattharasate, Charoonluk; Suzuki, Hiroshi; Nishikawa, Yoshifumi; Xuan, Xuenan. |
| Considering the scarce information on occurrences of Toxoplasma gondii and Neospora caninum in domestic animals from Turkey, the aim of this study was to investigate the seroprevalence of these parasite infections in cattle, horses, sheep, goats and dogs in Turkey. The specific antibodies against T gondii and N. caninum were detected by iELISAs based on the recombinant TgSAG2 or NcSAG1 in a total of 2,039 serum samples from eleven provinces. The seroprevalence of T. gondii infections was 46.3%, 4.0%, 20.0%, 12.9% and 19.8%, that of N. caninum infections was 0.3%, 7.4%, 2.1%, 3.2% and 16.6% in the horses, cattle, sheep, goats and dogs, respectively. These results indicated that T gondii and N. caninum infections are prevalent in Turkish domestic animals. |
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Palavras-chave: ELISA; Neospora caninum; Toxoplasma gondii; Turkey. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4422 |
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| Hara, Olgga A; Liao, Min; Baticados, Waren; Bannai, Hiroshi; Zhang, Guohong; Zhang, Sofa; Lee, Eung-goo; Nishikawa, Yoshifumi; Claveria, Florencia G.; Igarashi, Makoto; Nagasawa, Hideyuki; Xuan, Xuenan. |
| Neospora caninum infection is an important disease affecting bovine and canine populations worldwide. The dense granule antigen 7 of N. caninum (NcGRA7) is considered to be an immunodominant antigen. In the present study, the gene encoding truncated NcGRA7 (NcGRA7t) lacking an N-terminal signal peptide was expressed in Escherichia coli, and its diagnostic potential in an enzyme-linked immunosorbent assay (ELISA) was evaluated. The ELISA could clearly discriminate between known N. caninum-positive and -negative sera from dogs. Serum samples randomly collected from dogs in Japan and China were examined for the diagnosis of neosporosis using the ELISA. Twenty-three of 135 samples (17.03%) and 9 of 95 (9.47%) samples from Japan and China, respectively, were... |
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Palavras-chave: Canine; ELISA; Neospora caninum; NcGRA7. |
| Ano: 2006 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/995 |
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| Nishikawa, Yoshifumi. |
| Neosporosis is caused by the intracellular protozoan parasite Neospora caninum. This major disease-causing pathogen is responsible for inducing abortion in cattle, and these adverse events occur sporadically all over the world, including Japan. Currently, there are no vaccines on the market against infection with N. caninum. Because live and attenuated vaccines against N. caninum have had safety and effectiveness issues, development of a next-generation vaccine is urgently required. To develop a vaccine against neosporosis, my laboratory has been focused on the following: 1) understanding the host immune responses against Neospora infection, 2) identifying vaccine antigens and 3) developing an effective antigen-delivery system. The research strategy taken... |
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Palavras-chave: Adjuvant; Cattle; Neospora caninum; Neosporosis; Vaccine. |
| Ano: 2017 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4544 |
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| ICHIKAWA-SEKI, Madoka; GUSWANTO, Azirwan; ALLAMANDA, Puttik; MARIAMAH, Euis Siti; WIBOWO, Putut Eko; NISHIKAWA, Yoshifumi. |
| Neospora caninum can cause fetal abortion and neonatal mortality in cattle, and is a cause of economic concern worldwide. This study aimed to determine the prevalence of Neospora caninum-specific antibodies in cattle from Western Java, Indonesia. Serum samples from 991 cattle from 21 locations were tested for antibodies to N. caninum by using an enzyme-linked immunosorbent assay (ELISA) on the basis of recombinant NcSAG1. The overall seroprevalence was 16.6%, ranging from 0 to 87.5% in the sampled locations. The results of this study indicate latent infection rates of sampled animals were different in each location. Further studies are necessary to elucidate the relationship between N. caninum infection and abortion in cattle, and to identify risk factors... |
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Palavras-chave: Cattle; ELISA; Indonesia; NcSAG1; Neospora caninum. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4250 |
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| Zhang, Houshuang; Nishikawa, Yoshifumi; Ikehara, Yuzuru; Kojima, Naoya; Yokoyama, Naoaki; Xuan, Xuenan. |
| Liposomes coated with neoglycolipids constructed with mannopentaose and dipalmitoylphosphatidylethanolamine (Man3-DPPE), referred to as M3-DPPE liposomes, have been shown to induce cellular immunity against antigens encapsulated therein. To evaluate whether these M3-DPPE liposomes have an adjuvant capacity against Neospora caninum infection, a novel immunization method utilizing soluble N. caninum apical membrane antigen 1 (NcAMA1) encapsulated in the M3-DPPE liposomes (M3-NcAMA1) was employed. The intent was to reduce offspring mortality from N. caninum infection in susceptible, pregnant BALB/c mice. The results revealed that BALB/c mice developed IgG antibodies specific to N. caninum. A significant amount of interferon (IFN)-γ production was... |
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Palavras-chave: Neospora caninum; Apical membrane antigen 1; Oligomannose-coated liposomes. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2821 |
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| Dubey, J. P.. |
| Neospora caninum is a recently recognized protozoan parasite of animals. Until 1988, it was misdiagnosed as Toxoplasma gondii. Neospora caninum or Neospora-like parasites cause paralysis and death in dogs and neonatal mortality and abortion in cattle, sheep, goats and horses. Its life cycle is not known. Tachyzoites and tissue cysts are the only asexual stages known and the carnivorous definitive host is not known. Transplacental transmission is the only known natural route of infection. |
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Palavras-chave: Neospora caninum; Neosporosis; Tachyzoites; Tissue cysts; Paralysis; Abortion. |
| Ano: 1992 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/163 |
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| Fereig, Ragab M.; AbouLaila, Mahmoud Rezk; Mohamed, Samy G. A.; Mahmoud, Hassan Y. A. H.; Ali, Alsagher O.; Ali, Asmaa F.; Hilali, Mosaad; Zaid, Anis; Mohamed, Adel Elsayed Ahmed; Nishikawa, Yoshifumi. |
| Neospora caninum and Cryptosporidium parvum are intracellular protozoan parasites that are distributed worldwide and of major economical concern in cattle industry. N. caninum can cause abortion storms and high culling rates, whereas C. parvum has zoonotic implications and can cause diarrhea in calves. There are currently no data on the prevalence of neosporosis and cryptosporidiosis in humans or animals in southern Egypt. Prevalence of these two infections was determined in a sample of cattle from two different areas in southern Egypt, Sohag and Qena, using enzyme-linked immunosorbent assay. A total 301 cattle were sampled, of which 18.9% were positive for N. caninum, 35.9% were positive for C parvum and 10.0% were positive for both. Geographical location... |
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Palavras-chave: Cattle; Cryptosporidium parvum; Neospora caninum; ELISA; Egypt. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4531 |
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| Masuda, Tsuneyuki; Kobayashi, Yoshiyasu; Maeda, Ryuichiro; Omata, Yoshitaka. |
| CB-17 scid and BALB/c male mice were inoculated intraperitoneally with Neospora caninum to examine the possibility of its venereal transmission. Some of these mice were killed on days 7 and 20 post-inoculation to examine the genital organs for presence of the parasite. The remaining scid male mice were housed with non-infected female mice from day 7 p.i. and kept with them for 14 days. These scid mice died between days 28 and 35 p.i. N. caninum DNA was detected in the testis of mice on days 7 and 20 p.i. by PCR and tachyzoite viability was determined by bioassay conducted by means of mouse inoculation. Microscopically, fewer tachyzoites were detected in the testis obtained on day 20 p.i., than in other organs. The inoculated BALB/c male mice survived until... |
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Palavras-chave: CB-17 scid mouse; Neospora caninum; Venereal transmission. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1657 |
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| Ybañez, Rochelle Haidee D.; Leesombun, Arpron; Nishimura, Maki; Matsubara, Ryuma; Kojima, Mikiko; Sakakibara, Hitoshi; Nagamune, Kisaburo; Nishikawa, Yoshifumi. |
| Neospora caninum causes abortion and stillbirth in cattle. Identification of effective drugs against this parasite remains a challenge. Previous studies have suggested that disruption of abscisic acid (ABA)-mediated signaling in apicomplexan parasites such as Toxoplasma gondii offers a new drug target. In this study, the ABA inhibitor, fluridone (FLU), was evaluated for its action against N. caninum. Production of endogenous ABA within N. caninum was confirmed by ultra-performance liquid chromatography-tandem quadruple mass spectrometry. Subsequently, FLU treatment efficacy was assessed using in vitro. Results revealed that FLU inhibited the growth of N. caninum and T. gondii in vitro (IC50 143.1 +/- 43.96 mu M and 330.6 +/- 5238 mu M, respectively).... |
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Palavras-chave: Neospora caninum; Fluridone; Abscisic acid. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4491 |
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| Zhang, Houshuang; Lee, Eung-goo; Liao, Min; Compaore, Muller K.A.; Zhang, Guohong; Kawase, Osamu; Fujisaki, Kozo; Sugimoto, Chihiro; Nishikawa, Yoshifumi; Xuan, Xuenan. |
| The characterization of the cross-reactive antigens of two closely related apicomplexan parasites, Neospora caninum and Toxoplasma gondii, is important to elucidate the common mechanisms of parasite–host interactions. In this context, a gene encoding N. caninum ribosomal phosphoprotein P0 (NcP0) was identified by immunoscreening of a N. caninum tachyzoite cDNA expression library with antisera from mice immunized with T. gondii tachyzoites. The NcP0 was encoded by a gene with open reading frame of 936 bp, which encoded a protein of 311 amino acids. The NcP0 gene existed as a single copy in the genome and was interrupted by a 432 bp intron. The NcP0 showed 94.5% amino acid identity to T. gondii P0 (TgP0). Anti-recombinant NcP0 (rNcP0) sera recognized a... |
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Palavras-chave: Neospora caninum; Toxoplasma gondii; Ribosomal phosphoprotein P0; Cross-reactive. |
| Ano: 2007 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1035 |
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| Santana,Omar Iván; Cruz-Vázquez,Carlos; Medina-Esparza,Leticia; Ramos Parra,Miguel; Castellanos Morales,Ciro; Quezada Gallardo,Daniel. |
| El objetivo del trabajo fue detectar la presencia de ADN de N. caninum en animales infectados naturalmente, en dos tiempos de su primera gestación y al parto, así como registrar la presentación de abortos. Se seleccionó, en un establo, con presencia de parasitosis, un lote de 20 hembras de entre 12 y 14 meses de edad, seropositivas en ELISA, las hembras fueron inseminadas artificialmente y se tomaron muestras de sangre en el primero y segundo tercios de gestación y al parto; se aisló ADN y se sometió a PCR anidado en un solo tubo con iniciadores específicos. En el muestreo correspondiente al primer tercio de la gestación, se observaron 7/20 casos positivos (35%), en el segundo 15/20 (75%) y al parto 10/20 casos positivos (50%). De los animales incluidos en... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neospora caninum; ADN; Sangre; PCR; Ganado lechero; México. |
| Ano: 2010 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922010000200006 |
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| Motta Giraldo,J.L; Waltero García,Indira; Abeledo,Ma. Antonia; Fernández,O. |
| Se analizaron los resultados de las investigaciones serológicas para brucelosis, leptospirosis, herpesvirus bovino -1 (HVB-1), diarrea viral bovina (DVB) y Neospora caninum, de los casos enviados al laboratorio por los propietarios de los hatos bovinos en el departamento del Caquetá, Colombia, en los años 1999 - 2009. La información fue obtenida de la base de datos del ICA (Instituto Colombiano Agropecuario), seccional Caquetá. Se consideraron las variables: agentes infecciosos, años, municipios, zonas y sexos. El porcentaje promedio de brucelosis fue de 6,4% y los mayores valores se obtuvieron para herpesvirus bovino-1 (HVB-1). En el caso de Neospora, las investigaciones comenzaron en 2007 y en los 3 años solo se trabajaron 36 muestras, de las cuales 12... |
| Tipo: Journal article |
Palavras-chave: Brucellosis; Leptospirosis; Herpesvirus bovino-1; Diarrea viral bovina; Neospora caninum. |
| Ano: 2012 |
URL: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S0253-570X2012000300004 |
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| Registros recuperados: 43 | |
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