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	Provedor de dados BJPS (1) Autor Biggs,Chris (1) Mohiuddin,Omair Anwar (1) Palavra-chave Corticotrophin releasing factor (1) ND7/23 cells (1) Neuronal cells (1) RT-PCR (1) Urocortin (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2015 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Evaluation of the effect of natural peptide 'Urocortin' on corticotrophin releasing factor (CRF) receptor expression in ND7/23 cells BJPS Mohiuddin,Omair Anwar; Biggs,Chris. CRF receptors are involved in the stress management of the cells and are believed to have a cytoprotective role in the body. CRF receptors have been reported to be potential drug targets for the treatment of neurodegenerative disorders. The cell line used in the study is ND7/23 (mouse neuroblastoma and rat dorsal root ganglion neuron hybridoma). The aim of the study was to confirm the expression of CRF receptors in ND7/23 cells and to determine if urocortin (Ucn) can enhance the expression of CRF receptors. ND7/23 cells were cultured in RPMI 1640 media and cells grown after the second passage were used for the experiments. RNA was extracted from the cells and amplified by RT-PCR to confirm the presence of CRF receptors. The cells were then subjected to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Urocortin; ND7/23 cells; Corticotrophin releasing factor; RT-PCR; Neuronal cells. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502015000100233 Registros recuperados: 1 Primeira ... 1 ... Última

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