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Microsatellite markers developed through pyrosequencing allow clonal discrimination in the invasive alga Caulerpa taxifolia ArchiMer
Arnaud-haond, Sophie; Candeias, Rui; Serrao, Ester A.; Teixeira, Sara.
Polymorphic microsatellites were developed for the invasive green algae Caulerpa taxifolia using next generation DNA sequencing. Results showed a limited rate of microsatellites for the amount of sequences, possibly explaining failure of previous attempts for microsatellite development through classical methods. Eight polymorphic loci were selected that exhibited polymorphism and a null or negligible rate of amplification failure. The number of alleles per locus ranged from two to seven. The reconstruction of Multi Locus Genotypes and the heterozygosity and departure from Hardy-Weinberg equilibrium confirmed the influence of clonal reproduction and showed the usefulness of this set of markers to successfully discriminate clonal lineages and analyze the...
Tipo: Text Palavras-chave: Caulerpa taxifolia; Algae; Invasion; Mediterranean; Queensland; New South Wales; Seagrass.
Ano: 2013 URL: http://archimer.ifremer.fr/doc/00126/23720/21598.pdf
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Farming Systems in the Pastoral Zone of NSW: An Economic Analysis AgEcon
Khairo, Salahadin A.; Mullen, John D.; Hacker, Ronald B.; Patton, Dean A..
A ‘broad brush’ picture of farming in the pastoral zone of NSW is presented in this report. The pastoral zone of NSW is characterised by wide variations in climatic conditions, soil type and vegetation species. Hence representative faming system analysis was conducted for three sub-regions - the Upper Darling, the Murray-Darling and Far West. The regions were defined and described in terms of their resources, climate and the nature of agriculture. The main enterprises that farmers choose between were described and whole farm budgets and statements of assets and liabilities for the representative farms were developed. The representative farm models were used to compare traditional Merino based sheep enterprises with alternative sheep enterprises where meat...
Tipo: Report Palavras-chave: Western Division; Farming systems; Economic; Analysis; New South Wales; Farm Management; Land Economics/Use; Livestock Production/Industries; Production Economics; Research and Development/Tech Change/Emerging Technologies; Q160.
Ano: 2006 URL: http://purl.umn.edu/42655
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