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Le Guyader, Soizick; Atmar, Robert; Maalouf, Haifa; Le Pendu, Jacques. |
Shellfish can be a vector for human pathogens. Despite regulation based on enteric bacteria, shellfish are still implicated in viral outbreaks. Oysters are the most common shellfish associated with outbreaks, and noroviruses, which cause acute gastroenteritis, are the most frequently identified pathogen in these outbreaks. Analysis of shellfish-related outbreak data worldwide shows an unexpected high proportion of NoV GI strains. Recent studies performed in vitro, in vivo and in the environment indicate that oysters are not just a passive filter, but can selectively accumulate norovirus strains based on virus carbohydrate ligands shared with humans. These observations may help explain the GI/GII bias observed in shellfish-related outbreaks compared to... |
Tipo: Text |
Palavras-chave: Shellfish; Noroviruses; Oyster contamination; Pathogen. |
Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00150/26093/24313.pdf |
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Le Guyader, Soizick; Loisy, Fabienne; Atmar, R; Hutson, A; Estes, M; Ruvoen-clouet, N; Pommepuy, Monique; Le Pendu, J. |
The primary pathogens related to shellfishborne gastroenteritis outbreaks are noroviruses. These viruses show persistence in oysters, which suggests an active mechanism of virus concentration. We investigated whether Norwalk virus or viruslike particles bind specifically to oyster tissues after bioaccumulation or addition to tissue sections. Since noroviruses attach to carbohydrates of the histo-blood group family, tests using immunohistochemical analysis were performed to evaluate specific binding of virus or viruslike particles to oyster tissues through these ligands. Viral particles bind specifically to digestive ducts (midgut, main and secondary ducts, and tubules) by carbohydrate structures with a terminal N-acetylgalactosamine residue in an alpha... |
Tipo: Text |
Palavras-chave: Bioaccumulation; Shellfish; Escherichia coli; Pathogen; Oyster digestive tissue; Noroviruses. |
Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1542.pdf |
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Gallimore,C.I.; Barreiros,M.A.B.; Brown,D.W.G.; Nascimento,J.P.; Leite,J.P.G.. |
Noroviruses (Norwalk-like viruses) are an important cause of gastroenteritis worldwide. They are the most common cause of outbreaks of gastroenteritis in the adult population and occur in nursing homes for the elderly, geriatric wards, medical wards, and in hotel and restaurant settings. Food-borne outbreaks have also occurred following consumption of contaminated oysters. This study describes the application of a reverse transcription-polymerase chain reaction (RT-PCR) assay using random primers (PdN6) and specific Ni and E3 primers, directed at a small region of the RNA-dependent RNA polymerase-coding region of the norovirus genome, and DNA sequencing for the detection and preliminary characterisation of noroviruses in outbreaks of gastroenteritis in... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Noroviruses; Day care center; Gastroenteritis; Genotypes; Introduction. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000300005 |
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