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Hernandes,Silvio Evandro Daniel; Mello,Aline Cláudia de; Sant'Ana,Janete Jordão; Soares,Vanessa Sarto; Cassiolato,Valdir; Garcia,Lourdes Botelho; Cardoso,Celso Luíz. |
We compared the effectiveness of alcohol gel with that of the traditional hand-cleansing agents in removing clinical strains of Acinetobacter baumannii, methicillin-resistant Staphylococcus aureus, Escherichia coli, Enterococcus faecalis, Pseudomonas aeruginosa, and Candida albicans from artificially contaminated hands. The fingertips of 6 volunteers were contaminated with approximately 10(6) of microbial cells, and then were washed with: plain liquid soap, alcohol gel, 70% ethyl alcohol (by weight), 10% povidone-iodine liquid soap (PVP-I), and 4% chlorhexidine gluconate detergent. The experiments were performed using a Latin square statistical design, with six 6 x 5 randomized blocks, and the results were estimated by ANOVA. The products reduced from... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Alcohol gel; Hand-cleansing agents; Nosocomial pathogens. |
Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000100005 |
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Martínez,Pedro; Mattar,Salim. |
The purpose of this study was to identify the genes coding for resistance to ceftazidime and imipenem and describe the molecular epidemiology of A. baumannii strains isolated from a clinical center in Colombia. Twenty isolates of imipenem-resistant A. baumannii from an equal number of patients with nosocomial infections were obtained. Primers were used to amplify genes bla IMP, bla VIM, bla OXA-23, bla OXA-24, bla OXA-58, bla OXA-51 and bla ADC-7. To detect insertion sequences ISAba1/bla OXA-23, ISAba1/bla OXA-51 and ISAba1/bla ADC-7, mapping by PCR using combinations of reverse primers ISAba1 and reverse primers of bla OXA-23, bla OXA-51 and bla ADC-7 were used. The amplification products were purified and cloned into PCR 2.1-TOPO vector and transformed... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nosocomial pathogens; Antimicrobial resistance; PFGE. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400006 |
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