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The ability of mouse nuclear transfer embryonic stem cells to differentiate into primordial germ cells Genet. Mol. Biol.
Mansouri,Vahid; Salehi,Mohammad; Nourozian,Mohsen; Fadaei,Fatemeh; Farahani,Reza Mastery; Piryaei,Abbas; Delbari,Ali.
Nuclear transfer embryonic stem cells (ntESCs) show stem cell characteristics such as pluripotency but cause no immunological disorders. Although ntESCs are able to differentiate into somatic cells, the ability of ntESCs to differentiate into primordial germ cells (PGCs) has not been examined. In this work, we examined the capacity of mouse ntESCs to differentiate into PGCs in vitro. ntESCs aggregated to form embryoid bodies (EB) in EB culture medium supplemented with bone morphogenetic protein 4(BMP4) as the differentiation factor. The expression level of specific PGC genes was compared at days 4 and 8 using real time PCR. Flow cytometry and immunocytochemical staining were used to detect Mvh as a specific PGC marker. ntESCs expressed particular genes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Differentiation; Germ cells; Nuclear transfer embryonic stem cells.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000200220
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