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The case for sequencing the Pacific oyster genome ArchiMer
Hedgecock, Dennis; Gaffney, Patrick M.; Goulletquer, Philippe; Guo, Ximing; Reece, Kimberly; Warr, Gregory.
An international community of biologists presents the Pacific oyster Crassostrea gigas as a candidate for genome sequencing. This oyster has global distribution and for the past several years the highest annual production of any freshwater or marine organism (4.2 million metric tons, worth $3.5 billion US). Economic and cultural importance of oysters motivates a great deal of biologic research, which provides a compelling rationale for sequencing an oyster genome. Strong rationales for sequencing the oyster genome also come from contrasts to other genomes: membership in the Lophotrochozoa, an understudied branch of the Eukaryotes and high fecundity, with concomitantly high DNA sequence polymorphism and a population biology that is more like plants than any...
Tipo: Text Palavras-chave: Genomics; Evolutionary and ecological; Nucleotide diversity; Lophotrochozoa; Crassostrea gigas genome sequence; Pacific oyster.
Ano: 2005 URL: http://archimer.ifremer.fr/doc/2005/publication-2128.pdf
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Nucleotide diversity of a major carotenoid biosynthetic pathway gene in wild and cultivated Solanum (Section Lycopersicon) species Braz. J. Plant Physiol.
Araújo,Ana H.; Fonseca,Maria Esther de N.; Boiteux,Leonardo S..
A polymerase chain reaction (PCR)-based approach was employed to isolate putative alleles of the chromoplast-specific lycopene beta-cyclase (CYCB) gene from wild and cultivated tomatoes [Solanum (Section Lycopersicon)]. The objective of this work was to establish an effective PCR protocol by testing DNA samples from distinct germplasm accessions with a primer pair designed to selectively target conserved regions present in the available CYCB sequences. This PCR optimization allowed the amplification of 1219 out 1666 bp of the gene in six taxa: S. cheesmaniae, S. peruvianum, S. neorickii, S. pennellii, S. pimpinellifolium and S. lycopersicum. Sixty-three mutation sites (31 transitions, 18 transversions and 14 single base deletions/insertions) were detected...
Tipo: Info:eu-repo/semantics/other Palavras-chave: CYCB; Lycopene; Lycopene-beta-cyclase; Nucleotide diversity; Tomatoes; Molecular markers.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202007000300007
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