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Characterization of diesel degrading bacterial species from contaminated tropical ecosystem BABT
Nwinyi,Obinna Chukwuemeka; Kanu,Ijeoma Akudo; Tunde,Ayano; Ajanaku,Kolawole Oluseyi.
The bacterial diversity in a diesel contaminated tropical soil was investigated using diesel oxidation in gradient cultures dynamics (pH and OD) of the pure cultures. The diesel dependent growths of these isolates were assessed for 15 days by monitoring the gradient fluxes in the pH and Optical density OD of the media. Results showed an increase in OD as well as fluctuations in pH values. The mean OD data obtained was 0.515- 1.187 with pH of 6.95-7.2. From the morphological and biochemical characterization and comparison with respect to the standard references, the isolates S1P1, S3P3, S2P2, S2P1,and S3P2 were presumably the members of the genera Bacillus, Pseudomonas and Mycobacterium species. From the study, it was apparent that the tropical ecosystems...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Diesel; Optical density; Turbidity; Tropical Ecosystem; Bacterial strains; PH.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500789
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Measuring of Mycobacterium tuberculosis growth: a correlation of the optical measurements with colony forming units BJM
Peñuelas-Urquides,Katia; Villarreal-Treviño,Licet; Silva-Ramírez,Beatriz; Rivadeneyra-Espinoza,Liliana; Said-Fernández,Salvador; León,Mario Bermúdez de.
The quantification of colony forming units (cfu), turbidity, and optical density at 600 nm (OD600) measurements were used to evaluate Mycobacterium tuberculosis growth. Turbidity and OD600 measurements displayed similar growth curves, while cfu quantification showed a continuous growth curve. We determined the cfu equivalents to McFarland and OD600 units.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Bacterial growth; Colony forming units; McFarland; Mycobacterium tuberculosis; Optical density.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100042
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Comparison of methods for determining the color of Chilean honeys and the relationship of color with botanical origin in central Chile Ciencia e Investigación Agraria
Martin,María José; Fredes,Carolina; Nuñez,Gabriel; Ginocchio,Rosanna; Montenegro,Gloria.
The color of honey has special relevance in the international market, as different markets demand specific colors of honey. Chile has very diverse native and exotic flora, which are used by bees (Apis mellifera) to produce a range of honey types, including honeys of different colors. In this context, determining the relationships between color and botanical origin among Chilean honeys is very relevant. The objectives of the present study were to compare three methodologies for determining color in Chilean honeys (Pantone®, optical density (OD), and Pfund) and to define the relationship between color and botanical origin of the characterized honeys. The most common honeys from the Libertador Bernardo O'Higgins Region (VI Region) of Chile were Galega...
Tipo: Journal article Palavras-chave: Honey markets; Melissopalynological analysis; Optical density; Pantone®; Pfund.
Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202014000300014
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On the reliability of a simple method for scoring phenotypes to estimate heritability: A case study with pupal color in Heliconius erato phyllis , Fabricius 1775 (Lepidoptera, Nymphalidae) Genet. Mol. Biol.
Ferreira,Adriano Andrejew; Kucharski,Luiz Carlos; Araújo,Aldo Mellender de.
In this paper, two methods for assessing the degree of melanization of pupal exuviae from the butterfly Heliconius erato phyllis , Fabricius 1775 (Lepidoptera, Nymphalidae, Heliconiini) are compared. In the first method, which was qualitative, the exuviae were classified by scoring the degree of melanization, whereas in the second method, which was quantitative, the exuviae were classified by optical density followed by analysis with appropriate software. The heritability (h 2 ) of the degree of melanization was estimated by regression and analysis of variance. The estimates of h 2 were similar with both methods, indicating that the qualitative method could be particularly suitable for field work. The low estimates obtained for heritability may have...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Allocation-priority hypothesis; Butterfly; Optical density; Pupal melanization; Qualitative and quantitative methods.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100028
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