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Characterization and variations of organic parameters in teleost fish endolymph during day-night cycle, starvation and stress conditions ArchiMer
Guibbolini, M; Borelli, G; Mayer Gostan, N; Priouzeau, F; De Pontual, Helene; Allemand, D; Payan, P.
The aim of the present work was to examine the modifications of the organic composition of fish endolymph under environmental conditions (day-night cycle, starvation and Cl-2-Stress) known to modify otolith growth. Endolymph electrophoretic patterns were compared. An antibody raised against the trout otolith organic matrix allowed examining the variations of organic matrix precursors in the endolymph under the above conditions. Western blot analysis showed bands around 60-80 kDa. A 50% decrease of immunolabelling was observed during the night whereas increases were seen after starvation (factor 3) or stress (factor 2) suggesting that these variations could be related to the organic matrix deposit. A factor retarding in vitro CaCO3 crystallization (FRC) was...
Tipo: Text Palavras-chave: Turbot; Trout; Stress; Starvation; Day night cycle; Organic matrix; Otolith calcification; In vitro crystallization; Endolymph.
Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1875.pdf
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