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Rushdy,Abeer Ahmed; Mabrouk,Mona Ibrahim; Abu-Sef,Ferialla Abdel-Hamid; Kheiralla,Zeinab Hassan; Abdel -All,Said Mohamed; Saleh,Neveen Mohamed. |
OBJECTIVES: To study the potential factors include gene mutation, efflux pump and alteration of permeability associated with quinolone-resistance of Salmonella enterica strains isolated from patients with acute gastroenteritis and to evaluate the degree of synergistic activity of efflux pump inhibitors when combined with ciprofloxacin against resistant isolates. METHODS: Antimicrobial resistance patterns of fifty-eight Salmonella isolates were tested. Five isolates were selected to study the mechanism of resistance associated with quinolone group, including mutation in topoisomerase-encoding gene, altered cell permeability, and expression of an active efflux system. In addition, the combination between antibiotics and efflux pump inhibitors to overcome the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Fluoroquinolone resistance; Efflux pump; Outer membrane protein; Chromosomal mutations. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000400008 |
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Lafetá,Bárbara Nobre; Castro,Elaine Cristina de; Silva,Nivaldo da. |
The protein profile of the outer membrane of Leptospira interrogans serovar Hardjo subtype hardjoprajitno associated with the bovine natural immune response was investigated. The outer membrane proteins were extracted utilizing Triton X114 and precipitated with acetone. The protein sample was then resolved by SDS-PAGE and reacted in western blot against sera from a hyperimmune rabbit and from naturally infected bovines. In silver stained gels, 14 protein bands were observed, among which four proteins, with 22, 29, 47 and 63kDa, appeared as major constituents. Western blot tests with hyperimmune rabbit antiserum detected bands corresponding to proteins with 35; 27; 24; 21; 17 and 14kDa, while 32kDa and 45kDa proteins were the most immunoreactive with sera... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira interrogans; Outer membrane protein; Hardjoprajitno; Immunomodulatory proteins. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000900024 |
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