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Evaluation of ovarian structures using computerized microtomography Anais da ABC (AABC)
PAULINI,FERNANDA; CHAVES,SACHA B.; RÔLO,JOSÉ LUIZ J.P.; AZEVEDO,RICARDO B. DE; LUCCI,CAROLINA M..
ABSTRACT Visualization and clear understanding of the ovarian structures are important in determining the stage of oestrus, helping to diagnose several pathologies and supporting advances in reproductive technologies. In this research, computerized microtomography (microCT) was used to explore and characterize the ovarian structure of seven mammalian species. Ovaries of rats, female dog, queens, cows, mares, sows and a female donkey were used. After microCT scanning, the same samples were prepared for histologic evaluation, used here as a validation criterion. It was possible to distinguish regions of the cortex and medulla, visualize the morphology and distribution of blood vessels, clearly observe corpus luteum and antral follicles, and visualize oocytes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ovarian follicle; Blood vessels; Histology; Tomography; Ovary.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000502131
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Insulin effect during a Heatsynch protocol in dairy cows Ciência Rural
Maffi,Andressa Stein; Brauner,Cássio Cassal; Mielke,Lais Fernanda; Lima,Marcio Erpen; Xavier,Eduardo Gularte; Pino,Francisco Augusto Burkert Del; Corrêa,Marcio Nunes.
ABSTRACT: The objective of this study was to evaluate the effect of exogenous insulin administration on follicular growth, estrus presentation and conception rate during a protocol of ovulation synchronization. Dairy cows were subjected to the Heatsynch protocol, with the insertion of an intravaginal device containing 1.9 g of progesterone (CIDR) and an intramuscular injection containing 2.5 mg of GnRH on day 0. On day 7, the CIDR was removed and subjects were given 12.5 mg of dinoprost. Also on day 7, Insulin Group (IG, n = 21) animals received a subcutaneous injection containing 0.25 IU / kg of recombinant human insulin and the control group (CG, n = 25) received a 0.9% NaCl injection. On day 8, an injection of 0.5 mg of estradiol cypionate was...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dairy cow; Insulin; Ovarian follicle; Reproduction.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000800650
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Molecular and cellular mechanisms for the regulation of ovarian follicular function in cows OAK
SHIMIZU, Takashi; 清水, 隆.
Ovary is an important organ that houses the oocytes (reproductive cell). Oocyte growth depends on the function of follicular cells such as the granulosa and theca cells. Two-cell two gonadotropin systems are associated with oocyte growth and follicular cell functions. In addition to these systems, it is also known that several growth factors regulate oocyte growth and follicular cell functions. Vascular endothelial growth factor (VEGF) is involved in thecal vasculature during follicular development and the suppression of granulosa cell apoptosis. Metabolic factors such as insulin, growth hormone (GH) and insulin-like growth factor 1 (IGF-1) also play critical roles in the process of follicular development and growth. These factors are associated not only...
Palavras-chave: Endotoxin; Metabolic factors; Ovarian follicle; Steroid hormone; Vascular endothelial growth factor (VEGF).
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4325
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Evaluation and Validation of the Six Housekeeping Genes for Normalizing Mrna Expression in the Ovarian Follicles and Several Tissues in Chicken Rev. Bras. Ciênc. Avic.
Qin,N; Shan,X; Sun,X; Liswaniso,S; Chimbaka,IM; Xu,R.
ABSTRACT Expression of housekeeping genes is relatively constant in different tissues and cells by RT-qPCR analysis. Housekeeping genes (HGs) are usually utilized as the reference to evaluate and compare mRNA expression abundances of target genes in different cells or tissues sampled. However, the expression stabilities of different HGs in diverse samples may appear divergence. Currently, there is no exact reference data of HGs in hen ovarian follicular tissues during egg-laying period available yet. In this study, we detected the expression of 18SrRNA, ACTB, HOXC8, GAPDH, alpha-A, and alpha-D mRNA in the varied-size ovarian follicles (1-8 mm in diameter and F5), hearts, livers, spleens, lungs, and breast muscles of the laying hens by RT-qPCR, to analyze...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hen; Housekeeping gene; Ovarian follicle; Inner control.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000300302
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